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Open AccessArticle

Neutrophil Extracellular Traps in the Pathogenesis of Equine Recurrent Uveitis (ERU)

1
Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany
2
Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany
3
Clinic for Horses, University of Veterinary Medicine Hannover, Bünteweg 9, D-30559 Hannover, Germany
4
Tierärztliche Gemeinschaftspraxis für Pferde Wedemark, Lange Loh 15, D-30900 Wedemark, Germany
5
IVD Gesellschaft für Innovative Veterinärdiagnostik mbH (IVD GmbH), Albert-Einstein-Str. 5, D-30926 Seelze, Germany
6
Colzyx AB, Medicon Village, SE-223 81 Lund, Sweden
7
Chair of Animal Physiology, Department of Veterinary Sciences, LMU Munich, Lena Christ Str. 48, D-82152 Martinsried, Germany
8
Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands
9
Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany
*
Authors to whom correspondence should be addressed.
Cells 2019, 8(12), 1528; https://doi.org/10.3390/cells8121528
Received: 27 October 2019 / Revised: 18 November 2019 / Accepted: 22 November 2019 / Published: 27 November 2019
Equine recurrent uveitis (ERU) is considered one of the most important eye diseases in horses and typically appears with relapsing inflammatory episodes without systemic effects. Various disorders have been described as an initial trigger, including infections. Independent of the initiating cause, there are numerous indications that ERU is an immune-mediated disease. We investigated whether neutrophil extracellular traps (NETs) are part of the ERU pathogenesis. Therefore, vitreous body fluids (VBF), sera, and histological sections of the eye from ERU-diseased horses were analyzed for the presence of NET markers and compared with horses with healthy eyes. In addition, NET formation by blood derived neutrophils was investigated in the presence of VBF derived from horses with healthy eyes versus ERU-diseased horses using immunofluorescence microscopy. Interestingly, NET markers like free DNA, histone-complexes, and myeloperoxidase were detected in higher amounts in samples from ERU-diseased horses. Furthermore, in vitro NET formation was higher in neutrophils incubated with VBF from diseased horses compared with those animals with healthy eyes. Finally, we characterized the ability of equine cathelicidins to induce NETs, as potential NET inducing factors in ERU-diseased horses. In summary, our findings lead to the hypothesis that ERU-diseased horses develop more NETs and that these may contribute to the pathogenesis of ERU. View Full-Text
Keywords: NETs; equine recurrent uveitis; horse; cathelicidin NETs; equine recurrent uveitis; horse; cathelicidin
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Fingerhut, L.; Ohnesorge, B.; von Borstel, M.; Schumski, A.; Strutzberg-Minder, K.; Mörgelin, M.; Deeg, C.A.; Haagsman, H.P.; Beineke, A.; von Köckritz-Blickwede, M.; de Buhr, N. Neutrophil Extracellular Traps in the Pathogenesis of Equine Recurrent Uveitis (ERU). Cells 2019, 8, 1528.

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