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Open AccessArticle

Treatment with Human Amniotic Suspension Allograft Improves Tendon Healing in a Rat Model of Collagenase-Induced Tendinopathy

IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi 4, 20161 Milan, Italy
University Hospital São Paulo, Av. Prof. Lineu Prestes, 2565-Butantã, São Paulo, SP 05508-000, Brazil
Organogenesis, 2641 Rocky Ridge Lane, Birmingham, AL 35216, USA
Harvard Medical School, the Harvard Stem Cell Institute, and Department of Orthopedic Surgery, Brigham and Women’s Hospital, 7 Divinity Avenue, Cambridge, MA 02138, USA
Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021, USA
Authors to whom correspondence should be addressed.
Cells 2019, 8(11), 1411;
Received: 2 October 2019 / Revised: 31 October 2019 / Accepted: 4 November 2019 / Published: 8 November 2019
Treatment of tendon injuries is challenging, with neither conservative nor surgical approaches providing full recovery. Placental-derived tissues represent a promising tool for the treatment of tendon injuries. In this study, human amniotic suspension allograft (ASA) was investigated in a pre-clinical model of Achilles tendinopathy. Collagenase type I was injected in the right hind limb of Sprague Dawley rats to induce disease. Contralateral tendons were either left untreated or injected with saline as controls. Seven days following induction, tendons were injected with saline, ASA, or left untreated. Rats were sacrificed 14 and 28 days post-treatment. Histological and biomechanical analysis of tendons was completed. Fourteen days after ASA injection, improved fiber alignment and reduced cell density demonstrated improvement in degenerated tendons. Twenty-eight days post-treatment, tendons in all treatment groups showed fewer signs of degeneration, which is consistent with normal tendon healing. No statistically significant differences in histological or biomechanical analyses were observed between treatment groups at 28 days independent of the treatment they received. In this study, ASA treatment was safe, well-tolerated, and resulted in a widespread improvement of the tissue. The results of this study provide preliminary insights regarding the potential use of ASA for the treatment of Achilles tendinopathy. View Full-Text
Keywords: tendinopathy; amniotic membrane; collagenase; Achilles tendon; regenerative medicine tendinopathy; amniotic membrane; collagenase; Achilles tendon; regenerative medicine
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de Girolamo, L.; Morlin Ambra, L.F.; Perucca Orfei, C.; McQuilling, J.P.; Kimmerling, K.A.; Mowry, K.C.; Johnson, K.A.; Phan, A.T.; Whited, J.L.; Gomoll, A.H. Treatment with Human Amniotic Suspension Allograft Improves Tendon Healing in a Rat Model of Collagenase-Induced Tendinopathy. Cells 2019, 8, 1411.

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