Next Article in Journal
The Impact of Oxidative Stress on Ribosomes: From Injury to Regulation
Previous Article in Journal
Purine DNA Lesions at Different Oxygen Concentration in DNA Repair-Impaired Human Cells (EUE-siXPA)
Previous Article in Special Issue
A Low-Therapeutic Dose of Lithium Inhibits GSK3 and Enhances Myoblast Fusion in C2C12 Cells
Open AccessArticle

Inhibition of GSK3β Reduces Ectopic Lipid Accumulation and Induces Autophagy by the AMPK Pathway in Goat Muscle Satellite Cells

1
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
2
Institute of Liangshan Animal Husbandry and Veterinary Science, Xichang 615042, Sichuan, China
*
Author to whom correspondence should be addressed.
Cells 2019, 8(11), 1378; https://doi.org/10.3390/cells8111378
Received: 3 September 2019 / Revised: 27 October 2019 / Accepted: 31 October 2019 / Published: 1 November 2019
Ectopic lipid accumulation in muscle is important not only for obesity and myopathy treatment, but also for meat quality improvement in farm animals. However, the molecular mechanisms involved in lipid metabolism in muscle satellite cells are still elusive. In this study, SB216763 reduced GSK3β activation by increasing the level of pGSK3β (Ser9) and decreasing the level of total GSK3β protein. GSK3β inhibition decreased lipid accumulation and downregulated the expression level of lipogenesis-related genes in the adipogenic differentiation of goat muscle satellite cells. Furthermore, SB216763 treatment increased the levels of pAMPKα (T172) and pACC (Ser79). Further, we found that GSK3β inhibition promoted levels of LC3B-II and reduced the protein levels of p62 to induce the autophagy in muscle satellite cells. Taken together, our results provide new insight into a critical function for GSK3β: modulating lipid accumulation in goat muscle satellite cells through activating the AMPK pathway.
Keywords: GSK3β; muscle satellite cells; lipid accumulation; AMPK; autophagy GSK3β; muscle satellite cells; lipid accumulation; AMPK; autophagy
MDPI and ACS Style

Wang, L.; Liu, X.; Zhan, S.; Guo, J.; Yang, S.; Zhong, T.; Li, L.; Zhang, H.; Wang, Y. Inhibition of GSK3β Reduces Ectopic Lipid Accumulation and Induces Autophagy by the AMPK Pathway in Goat Muscle Satellite Cells. Cells 2019, 8, 1378.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop