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Open AccessArticle

Dendritic Cells Expressing MyD88 Molecule Are Necessary and Sufficient for CpG-Mediated Inhibition of IgE Production In Vivo

1
Institute of Biomedical Sciences, Department of Immunology, University of Sao Paulo, Sao Paulo 05508-000, Brazil
2
Institute of Molecular Medicine, University of Lisbon, 1649-028 Lisbon, Portugal
*
Author to whom correspondence should be addressed.
Cells 2019, 8(10), 1165; https://doi.org/10.3390/cells8101165
Received: 9 August 2019 / Revised: 23 September 2019 / Accepted: 25 September 2019 / Published: 28 September 2019
(This article belongs to the Special Issue The Molecular and Cellular Basis for Allergies & Asthma)
Elevated levels of immunoglobulin E (IgE) are associated with allergies and other immunological disorders. Sensitization with alum adjuvant favours IgE production while CpG-ODN adjuvant, a synthetic toll-like receptor 9 (TLR9) agonist, inhibits it. The cellular mechanisms underlying in vivo TLR regulation of immunoglobulin production, specially IgE, are still controversial. Specifically, TLR-mediated IgE regulation in vivo is not yet known. In this study we showed that augmented levels of IgE induced by sensitizations to OVA with or without alum adjuvant or with OVA-pulsed dendritic cells (DCs) were inhibited by co-administration of CpG. Notably, CpG-mediated suppression of IgE production required MyD88-expression on DCs but not on B-cells. This finding contrasts with previous in vitro studies reporting regulation of IgE by a direct action of CpG on B cells via MyD88 pathway. In addition, we showed that CpG also inhibited IgE production in a MyD88-dependent manner when sensitization was performed with OVA-pulsed DCs. Finally, CpG signalling through MyD88 pathway was also necessary and sufficient to prevent anaphylactic antibody production involved in active cutaneous anaphylaxis. View Full-Text
Keywords: allergy; IgE; IgG2c; anaphylaxis; dendritic cells allergy; IgE; IgG2c; anaphylaxis; dendritic cells
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MDPI and ACS Style

Alberca Custodio, R.W.; Mirotti, L.; Gomes, E.; Nunes, F.P.B.; S. Vieira, R.; Graça, L.; R. Almeida, R.; S. Câmara, N.O.; Russo, M. Dendritic Cells Expressing MyD88 Molecule Are Necessary and Sufficient for CpG-Mediated Inhibition of IgE Production In Vivo. Cells 2019, 8, 1165.

AMA Style

Alberca Custodio RW, Mirotti L, Gomes E, Nunes FPB, S. Vieira R, Graça L, R. Almeida R, S. Câmara NO, Russo M. Dendritic Cells Expressing MyD88 Molecule Are Necessary and Sufficient for CpG-Mediated Inhibition of IgE Production In Vivo. Cells. 2019; 8(10):1165.

Chicago/Turabian Style

Alberca Custodio, Ricardo W.; Mirotti, Luciana; Gomes, Eliane; Nunes, Fernanda P.B.; S. Vieira, Raquel; Graça, Luís; R. Almeida, Rafael; S. Câmara, Niels O.; Russo, Momtchilo. 2019. "Dendritic Cells Expressing MyD88 Molecule Are Necessary and Sufficient for CpG-Mediated Inhibition of IgE Production In Vivo" Cells 8, no. 10: 1165.

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