Next Article in Journal
Epigenetic Regulation of Inflammatory Cytokine-Induced Epithelial-To-Mesenchymal Cell Transition and Cancer Stem Cell Generation
Next Article in Special Issue
Turning the World Upside-Down in Cellulose for Improved Culturing and Imaging of Respiratory Challenges within a Human 3D Model
Previous Article in Journal
The Role of miRNAs in Immune Cell Development, Immune Cell Activation, and Tumor Immunity: With a Focus on Macrophages and Natural Killer Cells
Previous Article in Special Issue
Fisetin, a 3,7,3′,4′-Tetrahydroxyflavone Inhibits the PI3K/Akt/mTOR and MAPK Pathways and Ameliorates Psoriasis Pathology in 2D and 3D Organotypic Human Inflammatory Skin Models
Open AccessArticle

Modulation of the Lipid Profile of Reconstructed Skin Substitutes after Essential Fatty Acid Supplementation Affects Testosterone Permeability

1
Centre de Recherche en Organogénèse Expérimentale de l’Université Laval/LOEX, Québec, QC G1J 1Z4, Canada
2
Axe Médecine Régénératrice, Centre de Recherche du CHU de Québec–Université Laval, Québec, QC G1J 1Z4, Canada
3
Faculté de Pharmacie de l’Université Laval, Québec, QC G1V 0A6, Canada
4
Axe d’Endocrinologie et de Néphrologie, Centre de Recherche du CHU de Québec–Université Laval, Québec, QC G1V 4G2, Canada
5
Département de Chirurgie de l’Université Laval, Québec, QC G1V 0A6, Canada
*
Author to whom correspondence should be addressed.
Cells 2019, 8(10), 1142; https://doi.org/10.3390/cells8101142
Received: 13 August 2019 / Revised: 23 September 2019 / Accepted: 24 September 2019 / Published: 25 September 2019
Skin models with efficient skin barrier function are required for percutaneous absorption studies. The contribution of media supplementation with n-3 and n-6 polyunsaturated fatty acids (PUFAs) to the development of the skin barrier function of in vitro skin models remains incompletely understood. To investigate whether PUFAs, alpha-linolenic acid (ALA, n-3 PUFA) and linoleic acid (LA, n-6 PUFA), could enhance the impermeability of a three-dimensional reconstructed human skin model, skin substitutes were produced according to the self-assembly method using culture media supplemented with either 10 μM ALA or 10 μM LA. The impact of PUFAs on skin permeability was studied by using a Franz cell diffusion system to assess the percutaneous absorption of testosterone and benzoic acid. Our findings showed that ALA supplementation induced a decrease in the absorption of testosterone, while LA supplementation did not significantly influence the penetration of testosterone and benzoic acid under present experimental conditions. Both ALA and LA were incorporated into phospholipids of the skin substitutes, resulting in an increase in n-3 total PUFAs or n-6 total PUFAs. Collectively, these results revealed the under-estimated impact of n-3 PUFA supplementation as well as the importance of the n-6 to n-3 ratio on the formation of the skin barrier of in vitro reconstructed human skin models. View Full-Text
Keywords: skin substitutes; skin barrier function; polyunsaturated fatty acids; tissue engineering; lipidomics skin substitutes; skin barrier function; polyunsaturated fatty acids; tissue engineering; lipidomics
Show Figures

Figure 1

MDPI and ACS Style

Simard, M.; Julien, P.; Fradette, J.; Pouliot, R. Modulation of the Lipid Profile of Reconstructed Skin Substitutes after Essential Fatty Acid Supplementation Affects Testosterone Permeability. Cells 2019, 8, 1142.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop