Next Article in Journal
Ion Channels and Transporters in Inflammation: Special Focus on TRP Channels and TRPC6
Next Article in Special Issue
Challenges of Decoding Transcription Factor Dynamics in Terms of Gene Regulation
Previous Article in Journal
Metabolic Stress in the Immune Function of T Cells, Macrophages and Dendritic Cells
Previous Article in Special Issue
Real-Time Imaging of Retinal Ganglion Cell Apoptosis
Open AccessArticle

Tracking Cell Recruitment and Behavior within the Tumor Microenvironment Using Advanced Intravital Imaging Approaches

by 1, 1,2, 1,3,4,5,*,† and 1,6,*,†
1
Department of Microbiology, Immunology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada
2
National University of Science and Technology “MISIS”, Leninskiy Prospect 4, 119991 Moscow, Russia
3
Alberta Children’s Hospital Research Institute, Calgary, AB T2N 4N1, Canada
4
Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4N1, Canada
5
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada
6
Department of Critical Care Medicine, Faculty of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada
*
Authors to whom correspondence should be addressed.
Craig N. Jenne and Douglas J. Mahoney contributed equally to this work.
Cells 2018, 7(7), 69; https://doi.org/10.3390/cells7070069
Received: 9 May 2018 / Revised: 18 June 2018 / Accepted: 29 June 2018 / Published: 3 July 2018
(This article belongs to the Special Issue Innovative Methods to Monitor Single Live Cells)
Recent advances in imaging technology have made it possible to track cellular recruitment and behavior within the vasculature of living animals in real-time. Using approaches such as resonant scanning confocal and multiphoton intravital microscopy (IVM), we are now able to observe cells within the intact tumor microenvironment of a mouse. We are able to follow these cells for extended periods of time (hours) and can characterize how specific cell types (T cells, neutrophils, monocytes) interact with the tumor vasculature and cancer cells. This approach provides greater insight into specific cellular behaviors and cell–cell interactions than conventional techniques such as histology and flow cytometry. In this report, we describe the surgical preparation of animals to expose the tumor and both resonant scanning confocal and multiphoton imaging approaches used to track leukocyte recruitment, adhesion, and behavior within the tumor microenvironment. We present techniques for the measurement and quantification of leukocyte behavior within the bloodstream and tumor interstitium. The use of IVM to study leukocyte behavior within the tumor microenvironment provides key information not attainable with other approaches, that will help shape the development of better, more effective anticancer drugs and therapeutic approaches. View Full-Text
Keywords: intravital; cancer; vasculature; trafficking; leukocytes; imaging intravital; cancer; vasculature; trafficking; leukocytes; imaging
Show Figures

Figure 1

MDPI and ACS Style

Turk, M.; Naumenko, V.; Mahoney, D.J.; Jenne, C.N. Tracking Cell Recruitment and Behavior within the Tumor Microenvironment Using Advanced Intravital Imaging Approaches. Cells 2018, 7, 69. https://doi.org/10.3390/cells7070069

AMA Style

Turk M, Naumenko V, Mahoney DJ, Jenne CN. Tracking Cell Recruitment and Behavior within the Tumor Microenvironment Using Advanced Intravital Imaging Approaches. Cells. 2018; 7(7):69. https://doi.org/10.3390/cells7070069

Chicago/Turabian Style

Turk, Madison; Naumenko, Victor; Mahoney, Douglas J.; Jenne, Craig N. 2018. "Tracking Cell Recruitment and Behavior within the Tumor Microenvironment Using Advanced Intravital Imaging Approaches" Cells 7, no. 7: 69. https://doi.org/10.3390/cells7070069

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop