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Cells 2018, 7(4), 27; https://doi.org/10.3390/cells7040027

Natural Resistance Associated Macrophage Protein Is Involved in Immune Response of Blunt Snout Bream, Megalobrama amblycephala

1
Department of Cell Biology, School of Life Sciences, Central South University, Changsha 410017, China
2
Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 18 February 2018 / Revised: 7 March 2018 / Accepted: 24 March 2018 / Published: 29 March 2018
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Abstract

The natural resistance-associated macrophage protein gene (Nramp), has been identified as one of the significant candidate genes responsible for modulating vertebrate natural resistance to intracellular pathogens. Here, we identified and characterized a new Nramp family member, named as maNramp, in the blunt snout bream. The full-length cDNA of maNramp consists of a 153 bp 5′UTR, a 1635 bp open reading frame encoding a protein with 544 amino acids, and a 1359 bp 3′UTR. The deduced protein (maNRAMP) possesses the typical structural features of NRAMP protein family, including 12 transmembrane domains, three N-linked glycosylation sites, and a conserved transport motif. Phylogenetic analysis revealed that maNRAMP shares the significant sequence consistency with other teleosts, and shows the higher sequence similarity to mammalian Nramp2 than Nramp1. It was found that maNramp expressed ubiquitously in all normal tissues tested, with the highest abundance in the spleen, followed by the head kidney and intestine, and less abundance in the muscle, gill, and kidney. After lipopolysaccharide (LPS) stimulation, the mRNA level of maNramp was rapidly up-regulated, which reached a peak level at 6 h. Altogether, these results indicated that maNramp might be related to fish innate immunity and similar to mammalian Nramp1 in function. View Full-Text
Keywords: lipopolysaccharide stimulation; Megalobrama amblycephala; innate immune; inflammation; natural resistance-associated macrophage protein lipopolysaccharide stimulation; Megalobrama amblycephala; innate immune; inflammation; natural resistance-associated macrophage protein
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Jiang, Y.-H.; Mao, Y.; Lv, Y.-N.; Tang, L.-L.; Zhou, Y.; Zhong, H.; Xiao, J.; Yan, J.-P. Natural Resistance Associated Macrophage Protein Is Involved in Immune Response of Blunt Snout Bream, Megalobrama amblycephala. Cells 2018, 7, 27.

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