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Cells
Volume 14
Issue 9
10.3390/cells14090656
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway

by
Wei Zhang
1,†,
Minying Zhang
1,†,
Jiaqing Zhang
2,
Sujuan Chen
3,
Keke Zhang
1,
Xuejing Xie
1,
Chaofan Guo
1,
Jiyuan Shen
1,
Xiaojian Zhang
1,
Huarun Sun
1,
Liya Guo
1,
Yuliang Wen
1,
Lei Wang
1,* and
Jianhe Hu
1,*
1
Henan Institute of Science and Technology, College of Animal Science and Veterinary Medicine, Xinxiang 453003, China
2
Institute of Animal Husbandry, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
3
School of Life Sciences and Technology, Xinxiang Medical University, Xinxiang 453003, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Cells 2025, 14(9), 656; https://doi.org/10.3390/cells14090656 (registering DOI)
Submission received: 23 March 2025 / Revised: 13 April 2025 / Accepted: 16 April 2025 / Published: 29 April 2025
(This article belongs to the Section Cell Signaling)
Versions Notes

Abstract

The myogenic differentiation of muscle satellite cells (MuSCs) is an important biological process that plays a key role in the regeneration and repair of skeletal muscles. However, the mechanisms regulating myoblast myogenesis require further investigation. In this study, we found that STEAP3 is involved in myogenic differentiation based on the Yunan black pig MuSCs model in vitro using cell transfection and other methods. Furthermore, the expression of myogenic differentiation marker genes MyoG and MyoD and the number of myotubes formed by the differentiation of cells from the si-STEAP3 treated group were significantly decreased but increased in the STEAP3 overexpression group compared to that in the control group. STEAP3 played a role in iron ion metabolism, affecting myogenic differentiation via the uptake of iron ions and enhancing IRP-IRE homeostasis. STEAP3 also activated the PI3K/AKT pathway, thus promoting myoblast differentiation of Yunan black pig MuSCs. The results of this study showed that STEAP3 overexpression increased intracellular iron ion content and activated the homeostatic IRP-IRE system to regulate intracellular iron ion metabolism.
Keywords: STEAP3; MuSCs myogenic differentiation; iron homeostasis; PI3K/AKT pathway STEAP3; MuSCs myogenic differentiation; iron homeostasis; PI3K/AKT pathway

Share and Cite

MDPI and ACS Style

Zhang, W.; Zhang, M.; Zhang, J.; Chen, S.; Zhang, K.; Xie, X.; Guo, C.; Shen, J.; Zhang, X.; Sun, H.; et al. The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway. Cells 2025, 14, 656. https://doi.org/10.3390/cells14090656

AMA Style

Zhang W, Zhang M, Zhang J, Chen S, Zhang K, Xie X, Guo C, Shen J, Zhang X, Sun H, et al. The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway. Cells. 2025; 14(9):656. https://doi.org/10.3390/cells14090656

Chicago/Turabian Style

Zhang, Wei, Minying Zhang, Jiaqing Zhang, Sujuan Chen, Keke Zhang, Xuejing Xie, Chaofan Guo, Jiyuan Shen, Xiaojian Zhang, Huarun Sun, and et al. 2025. "The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway" Cells 14, no. 9: 656. https://doi.org/10.3390/cells14090656

APA Style

Zhang, W., Zhang, M., Zhang, J., Chen, S., Zhang, K., Xie, X., Guo, C., Shen, J., Zhang, X., Sun, H., Guo, L., Wen, Y., Wang, L., & Hu, J. (2025). The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway. Cells, 14(9), 656. https://doi.org/10.3390/cells14090656

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