Purified Cornel Iridoid Glycosides Attenuated Oxidative Stress Induced by Cerebral Ischemia-Reperfusion Injury via Morroniside and Loganin Targeting Nrf2/NQO-1/HO-1 Signaling Pathway
Abstract
1. Introduction
2. Materials and Methods
2.1. Drugs
2.2. Animal, Middle Cerebral Artery Occlusion Injury (MCAO/R) and Drug Treatment
2.3. Neurological Deficit
2.4. 2,3,5-Triphenyltetrazolium Hydrochloride (TTC) Staining
2.5. Brain Water Content Measurement
2.6. H&E and TUNEL Staining
2.7. Cell Culture and Treatment
2.8. MTT Assays
2.9. Determination of ROS Levels
2.10. Determination of Mitochondrial Function
2.11. Immunofluorescence Detection
2.12. Western Blotting
2.13. Biochemical Analysis
2.14. Molecular Docking
2.15. Statistical Analysis
3. Results
3.1. Protected the Rats Against MCAO/R-Induced Brain Damages
3.2. Improvement on Histopathological Changes in MCAO/R Rats
3.3. Enhanced the Antioxidant Capacity of MCAO/R Rats
3.4. Protected PC12 Cells Against H2O2-Induced Cell Apoptosis
3.5. Enhanced Activities on Scavenging ROS
3.6. Activated Signaling Pathways of Nrf2/NQO-1 and Nrf2/HO-1
3.7. Molecular Docking Studies of Morroniside and Loganin with Keap1
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Binding Energy (kcal/mol) | Hydrogen Bonding Interactions | Distance (A°) | |
---|---|---|---|
Morroniside | −5.31 | GLY367 | 3.09 |
VAL420 | 2.47, 2.20 | ||
VAL465 | 2.60 | ||
VAL512 | 2.22, 1.69 | ||
VAL606 | 2.20 | ||
Loganin | −6.54 | GLY367 | 2.42, 2.56 |
ILE559 | 1.96 | ||
LEU365 | 2.83, 2.82 | ||
THR560 | 2.72 | ||
VAL418 | 2.65 | ||
VAL465 | 2.23 | ||
VAL561 | 2.75 | ||
VAL604 | 2.96 | ||
VAL606 | 2.06, 2.44 |
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Wang, Z.; Xue, F.; Hu, E.; Wang, Y.; Li, H.; Qiao, B. Purified Cornel Iridoid Glycosides Attenuated Oxidative Stress Induced by Cerebral Ischemia-Reperfusion Injury via Morroniside and Loganin Targeting Nrf2/NQO-1/HO-1 Signaling Pathway. Cells 2025, 14, 1205. https://doi.org/10.3390/cells14151205
Wang Z, Xue F, Hu E, Wang Y, Li H, Qiao B. Purified Cornel Iridoid Glycosides Attenuated Oxidative Stress Induced by Cerebral Ischemia-Reperfusion Injury via Morroniside and Loganin Targeting Nrf2/NQO-1/HO-1 Signaling Pathway. Cells. 2025; 14(15):1205. https://doi.org/10.3390/cells14151205
Chicago/Turabian StyleWang, Zhaoyang, Fangli Xue, Enjie Hu, Yourui Wang, Huiliang Li, and Boling Qiao. 2025. "Purified Cornel Iridoid Glycosides Attenuated Oxidative Stress Induced by Cerebral Ischemia-Reperfusion Injury via Morroniside and Loganin Targeting Nrf2/NQO-1/HO-1 Signaling Pathway" Cells 14, no. 15: 1205. https://doi.org/10.3390/cells14151205
APA StyleWang, Z., Xue, F., Hu, E., Wang, Y., Li, H., & Qiao, B. (2025). Purified Cornel Iridoid Glycosides Attenuated Oxidative Stress Induced by Cerebral Ischemia-Reperfusion Injury via Morroniside and Loganin Targeting Nrf2/NQO-1/HO-1 Signaling Pathway. Cells, 14(15), 1205. https://doi.org/10.3390/cells14151205