JAK2/STAT3 Signaling in Myeloid Cells Contributes to Obesity-Induced Inflammation and Insulin Resistance
Abstract
1. Introduction
2. Materials and Methods
2.1. Mice and Treatment
2.2. Immune Cell Isolation and Flow Cytometry
2.3. Histology and Immunohistochemistry (IHC)
2.4. Free Fatty Acid (FFA) Treatment
2.5. Measurement of FFA, Insulin, and Triglyceride Concentration
2.6. Measurement of IL-6 and CCL2 Concentration
2.7. Quantitative Real-Time PCR
2.8. Phospho-ELISA
2.9. Statistical Analysis
3. Results
3.1. Myeloid Cell Jak2 Promotes Obesity-Induced Insulin Resistance
3.1.1. Jak2 Promotes M1 Polarization of ATMs During Obesity
3.1.2. Jak2–Stat3 Pathway Mediates FFA-Induced Inflammation
3.1.3. Stat3 Is a Target to Ameliorate Obesity-Induced Insulin Resistance
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhang, C.; Song, J.; Zhang, W.; Huang, R.; Li, Y.-J.; Zhang, Z.; Xin, H.; Zhao, Q.; Li, W.; Priceman, S.J.; et al. JAK2/STAT3 Signaling in Myeloid Cells Contributes to Obesity-Induced Inflammation and Insulin Resistance. Cells 2025, 14, 1194. https://doi.org/10.3390/cells14151194
Zhang C, Song J, Zhang W, Huang R, Li Y-J, Zhang Z, Xin H, Zhao Q, Li W, Priceman SJ, et al. JAK2/STAT3 Signaling in Myeloid Cells Contributes to Obesity-Induced Inflammation and Insulin Resistance. Cells. 2025; 14(15):1194. https://doi.org/10.3390/cells14151194
Chicago/Turabian StyleZhang, Chunyan, Jieun Song, Wang Zhang, Rui Huang, Yi-Jia Li, Zhifang Zhang, Hong Xin, Qianqian Zhao, Wenzhao Li, Saul J. Priceman, and et al. 2025. "JAK2/STAT3 Signaling in Myeloid Cells Contributes to Obesity-Induced Inflammation and Insulin Resistance" Cells 14, no. 15: 1194. https://doi.org/10.3390/cells14151194
APA StyleZhang, C., Song, J., Zhang, W., Huang, R., Li, Y.-J., Zhang, Z., Xin, H., Zhao, Q., Li, W., Priceman, S. J., Deng, J., Liu, Y., Ann, D., Seewaldt, V., & Yu, H. (2025). JAK2/STAT3 Signaling in Myeloid Cells Contributes to Obesity-Induced Inflammation and Insulin Resistance. Cells, 14(15), 1194. https://doi.org/10.3390/cells14151194