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Article

miRNA-Driven Regulation of Endothelial-to-Mesenchymal Transition Differs among Thoracic Aortic Aneurysms

by
Sonia Terriaca
1,
Maria Giovanna Scioli
2,*,
Fabio Bertoldo
3,
Calogera Pisano
3,
Paolo Nardi
3,
Carmela Rita Balistreri
4,
Daniele Magro
4,
Beatrice Belmonte
5,6,
Luca Savino
1,
Amedeo Ferlosio
2,† and
Augusto Orlandi
2,7,†
1
Anatomic Pathology, Fondazione Policlinico Tor Vergata, 00133 Rome, Italy
2
Anatomic Pathology, Department of Biomedicine and Prevention, Tor Vergata University, 00133 Rome, Italy
3
Cardiac Surgery Unit, Department of Surgery, Tor Vergata University, 00133 Rome, Italy
4
Cellular and Molecular Laboratory, Department of Biomedicine, Neuroscience and Advanced Diagnostics (Bi.N.D.), University of Palermo, 90134 Palermo, Italy
5
Tumor Immunology Unit, Department of Health Sciences, University of Palermo, 90134 Palermo, Italy
6
Azienda sanitaria Provinciale di Catania (ASP), 95124 Catania, Italy
7
Department of Biomedical Sciences, Catholic University Our Lady of Good Counsel, 1001 Tirana, Albania
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Cells 2024, 13(15), 1252; https://doi.org/10.3390/cells13151252
Submission received: 30 May 2024 / Revised: 16 July 2024 / Accepted: 24 July 2024 / Published: 25 July 2024
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Abstract

Thoracic aortic aneurysms (TAAs) represent a serious health concern, as they are associated with early aortic dissection and rupture. TAA formation is triggered by genetic conditions, in particular Marfan syndrome (MFS) and bicuspid aortic valve (BAV). During the aneurysmatic process, aortic endothelial cells can undergo endothelial-to-mesenchymal transition (End–MT) with consequent phenotypic and functional alterations. We previously documented that MFS TAA is characterized by miR-632–driven End–MT exacerbation, whereas in BAV aortopathy, the occurrence of this process remains still controversial. We investigated the End–MT process and the underlined regulatory mechanisms in BAV, TAV and MFS TAA tissues. Gene expression and immunohistochemical analysis were performed in order to analyze some important miRNAs and genes characterizing End–MT. We documented that BAV endothelium maintains the expression of the endothelial homeostasis markers, such as ERG, CD31 and miR-126-5p, while it shows lower levels of miR-632 and mesenchymal markers compared with MFS. Interestingly, we also found higher levels of miR-632 in MFS patients’ blood. Our findings definitively demonstrate that the End–MT process does not characterize BAV that, among the other TAAs, better maintains the endothelial features. In addition, our results suggest miR-632 as a promising diagnostic/prognostic factor in MFS aortopathy.
Keywords: thoracic aortic aneurysms; Marfan syndrome; bicuspid aortic valve; endothelial cells; endothelial-to-mesenchymal transition; tissue and circulating miRNAs; miR-632; miR-126-5p thoracic aortic aneurysms; Marfan syndrome; bicuspid aortic valve; endothelial cells; endothelial-to-mesenchymal transition; tissue and circulating miRNAs; miR-632; miR-126-5p

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MDPI and ACS Style

Terriaca, S.; Scioli, M.G.; Bertoldo, F.; Pisano, C.; Nardi, P.; Balistreri, C.R.; Magro, D.; Belmonte, B.; Savino, L.; Ferlosio, A.; et al. miRNA-Driven Regulation of Endothelial-to-Mesenchymal Transition Differs among Thoracic Aortic Aneurysms. Cells 2024, 13, 1252. https://doi.org/10.3390/cells13151252

AMA Style

Terriaca S, Scioli MG, Bertoldo F, Pisano C, Nardi P, Balistreri CR, Magro D, Belmonte B, Savino L, Ferlosio A, et al. miRNA-Driven Regulation of Endothelial-to-Mesenchymal Transition Differs among Thoracic Aortic Aneurysms. Cells. 2024; 13(15):1252. https://doi.org/10.3390/cells13151252

Chicago/Turabian Style

Terriaca, Sonia, Maria Giovanna Scioli, Fabio Bertoldo, Calogera Pisano, Paolo Nardi, Carmela Rita Balistreri, Daniele Magro, Beatrice Belmonte, Luca Savino, Amedeo Ferlosio, and et al. 2024. "miRNA-Driven Regulation of Endothelial-to-Mesenchymal Transition Differs among Thoracic Aortic Aneurysms" Cells 13, no. 15: 1252. https://doi.org/10.3390/cells13151252

APA Style

Terriaca, S., Scioli, M. G., Bertoldo, F., Pisano, C., Nardi, P., Balistreri, C. R., Magro, D., Belmonte, B., Savino, L., Ferlosio, A., & Orlandi, A. (2024). miRNA-Driven Regulation of Endothelial-to-Mesenchymal Transition Differs among Thoracic Aortic Aneurysms. Cells, 13(15), 1252. https://doi.org/10.3390/cells13151252

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