New Insights into the Impact of Human Papillomavirus on Oral Cancer in Young Patients: Proteomic Approach Reveals a Novel Role for S100A8
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Population
2.2. Laser-Capture Microdissection and Protein Extraction
2.3. Liquid Chromatography Coupled with Tandem Mass Spectrometry (LC-MS/MS)
2.4. LC-MS/MS Data Analysis
2.5. Bioinformatics Analysis
2.6. Cell Lines and TLR Activation
2.7. Immunofluorescence
2.8. Flow Cytometry
2.9. Statistical Analysis
3. Results
3.1. Determination of Differently Expressed Proteins between HPV (+) and HPV (−) OSCC through Discovery of Proteomics
3.2. High Expression of S100A8 Is Correlated with Advanced Clinical Stage and Worse Survival Rate
3.3. OSCCs with S100A8 Overexpression Exhibit Lower Amounts of M1 Macrophages and Dendritic Cells
3.4. S100A8 Is Overexpressed in HPV-Associated OSCC
3.5. HPV-Positive Cells Increase S100A8 and NFκB Activation upon Stimulation of Pattern Recognition Receptor TLR4
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Miranda-Galvis, M.; Carneiro Soares, C.; Moretto Carnielli, C.; Ramalho Buttura, J.; Sales de Sá, R.; Kaminagakura, E.; Marchi, F.A.; Paes Leme, A.F.; Lópes Pinto, C.A.; Santos-Silva, A.R.; et al. New Insights into the Impact of Human Papillomavirus on Oral Cancer in Young Patients: Proteomic Approach Reveals a Novel Role for S100A8. Cells 2023, 12, 1323. https://doi.org/10.3390/cells12091323
Miranda-Galvis M, Carneiro Soares C, Moretto Carnielli C, Ramalho Buttura J, Sales de Sá R, Kaminagakura E, Marchi FA, Paes Leme AF, Lópes Pinto CA, Santos-Silva AR, et al. New Insights into the Impact of Human Papillomavirus on Oral Cancer in Young Patients: Proteomic Approach Reveals a Novel Role for S100A8. Cells. 2023; 12(9):1323. https://doi.org/10.3390/cells12091323
Chicago/Turabian StyleMiranda-Galvis, Marisol, Carolina Carneiro Soares, Carolina Moretto Carnielli, Jaqueline Ramalho Buttura, Raisa Sales de Sá, Estela Kaminagakura, Fabio Albuquerque Marchi, Adriana Franco Paes Leme, Clóvis A. Lópes Pinto, Alan Roger Santos-Silva, and et al. 2023. "New Insights into the Impact of Human Papillomavirus on Oral Cancer in Young Patients: Proteomic Approach Reveals a Novel Role for S100A8" Cells 12, no. 9: 1323. https://doi.org/10.3390/cells12091323