Proteomic Characterization of SAS Cell-Derived Extracellular Vesicles in Relation to Both BPA and Neutron Irradiation Doses
Abstract
:1. Introduction
2. Materials and Methods
2.1. Neutron Irradiation of SAS Cells
2.2. Cell Growth Assay
2.3. EVs Isolation from Cell Medium
2.4. LC-MS/MS Analysis of SAS Cells Medium-Derived EVs
2.4.1. In-Solution Digestion
2.4.2. Liquid Chromatography
2.4.3. Mass Spectrometry
2.5. Proteomic Data Processing and Data Mining
2.6. Network Analysis
3. Results
3.1. Evaluation of Cellular Growth and Viability after BNCT Treatment
3.2. Proteomics Profiles of EVs under Different Conditions
3.3. Differential Analysis of EVs between BPA+ and BPA− Conditions and within BPA+ Conditions between Different Times and Doses of Neutron Irradiation
3.4. Network Analysis Shows the Major Pathways Involved in BNCT Effect and How They Are Connected
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Irradiated Time (min) | Inoculated Cell Numbers | Cell Growth Ratio * | |
---|---|---|---|
BPA+ | BPA− | ||
0 | 1000 | 1.00 ± 0.04 | 1.00 ± 0.11 |
10 | 1000 | 0.40 ± 0.20 | 1.11 ± 0.15 |
60 | 5000 | <6.5 × 10−4 | 0.10 ± 0.04 |
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© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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Perico, D.; Tong, Y.; Chen, L.; Imamichi, S.; Sanada, Y.; Ishiai, M.; Suzuki, M.; Masutani, M.; Mauri, P. Proteomic Characterization of SAS Cell-Derived Extracellular Vesicles in Relation to Both BPA and Neutron Irradiation Doses. Cells 2023, 12, 1562. https://doi.org/10.3390/cells12121562
Perico D, Tong Y, Chen L, Imamichi S, Sanada Y, Ishiai M, Suzuki M, Masutani M, Mauri P. Proteomic Characterization of SAS Cell-Derived Extracellular Vesicles in Relation to Both BPA and Neutron Irradiation Doses. Cells. 2023; 12(12):1562. https://doi.org/10.3390/cells12121562
Chicago/Turabian StylePerico, Davide, Ying Tong, Lichao Chen, Shoji Imamichi, Yu Sanada, Masamichi Ishiai, Minoru Suzuki, Mitsuko Masutani, and Pierluigi Mauri. 2023. "Proteomic Characterization of SAS Cell-Derived Extracellular Vesicles in Relation to Both BPA and Neutron Irradiation Doses" Cells 12, no. 12: 1562. https://doi.org/10.3390/cells12121562