Short-Term Autophagy Preconditioning Upregulates the Expression of COX2 and PGE2 and Alters the Immune Phenotype of Human Adipose-Derived Stem Cells In Vitro
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Cell Culture
2.2. Western Blot Analysis
2.3. Flow Cytometry
2.4. Colony Forming Unit-Fibroblast (CFU-F) Assay
2.5. RNA Isolation and Quantitative Reverse-Transcription PCR (qRT-PCR)
2.6. Enzyme-Linked Immunosorbent Assay (ELISA)
2.7. Statistical Analysis
3. Results
3.1. The mTOR Inhibitor Rapamycin Induces, While the PI3K Inhibitor 3-MA Suppresses, Autophagy in hASCs
3.2. Autophagy Preconditioning Does Not Alter hASCs Stem Cell Properties
3.3. Autophagy Preconditioning of hASCs Alters Expression of Both Anti-Inflammatory and Pro-Inflammatory Mediators
3.4. Autophagy Preconditioning of hASCs Alters Response to Pro-Inflammatory Stimulation with hIFNγ
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Donor | Age | BMI |
---|---|---|
1 | 34 | 20.34 |
2 | 40 | 21.18 |
3 | 39 | 23.4 |
4 | 25 | 22.0 |
5 | 40 | 21.19 |
Average ± SD | 36.5 ± 2.87 | 21.62 ± 0.52 |
Gene | Forward (5′-3′) | Reverse (5′-3′) |
---|---|---|
Beta-actin | ACGTTGCTATCCAGGCTGTGCTAT | TTAATGTCACGCACGATTTCCCGC |
ATG7 | ATGATCCCTGTAACTTAGCCCA | CACGGAAGCAAACAACTTCAAC |
LC3B | AAGGCGCTTACAGCTCAATG | CTGGGAGGCATAGACCATGT |
P62 | GCACCCCAATGTGATCTGC | CGCTACACAAGTCGTAGTCTGG |
TGF-β | CAGTCACCATAGCAACACTC | CCTGGCCTGAACTACTATCT |
IDO | TCTCATTTCGTGATGGAGACTGC | GTGTCCCGTTCTTGCATTTGC |
TSG-6 | AGAATTTGTGAGCAGCCCCT | GGCTGCTCGTTCAAGCCATA |
IL-1β | CATGGGATAACGAGGCTTATG | CCACTTGTTGCTCCATATCC |
IL-6 | CCTTCCAAAGATGGCTGAAA | TGGCTTGTTCCTCACTACT |
COX2 | TTGCTGGCAGGGTTGCTGGTGGTA | CATCTGCCTGCTCTGGTCAATCGAA |
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Wise, R.M.; Al-Ghadban, S.; Harrison, M.A.A.; Sullivan, B.N.; Monaco, E.R.; Aleman, S.J.; Donato, U.M.; Bunnell, B.A. Short-Term Autophagy Preconditioning Upregulates the Expression of COX2 and PGE2 and Alters the Immune Phenotype of Human Adipose-Derived Stem Cells In Vitro. Cells 2022, 11, 1376. https://doi.org/10.3390/cells11091376
Wise RM, Al-Ghadban S, Harrison MAA, Sullivan BN, Monaco ER, Aleman SJ, Donato UM, Bunnell BA. Short-Term Autophagy Preconditioning Upregulates the Expression of COX2 and PGE2 and Alters the Immune Phenotype of Human Adipose-Derived Stem Cells In Vitro. Cells. 2022; 11(9):1376. https://doi.org/10.3390/cells11091376
Chicago/Turabian StyleWise, Rachel M., Sara Al-Ghadban, Mark A. A. Harrison, Brianne N. Sullivan, Emily R. Monaco, Sarah J. Aleman, Umberto M. Donato, and Bruce A. Bunnell. 2022. "Short-Term Autophagy Preconditioning Upregulates the Expression of COX2 and PGE2 and Alters the Immune Phenotype of Human Adipose-Derived Stem Cells In Vitro" Cells 11, no. 9: 1376. https://doi.org/10.3390/cells11091376