Interspecies Diversity of Osmotic Gradient Deformability of Red Blood Cells in Human and Seven Vertebrate Animal Species
Abstract
:1. Introduction
2. Materials and Methods
2.1. Volunteer Participants, Experimental Animals, and Blood Sampling
2.2. Hematological Measurements
2.3. Red Blood Cell Deformability (Conventional and Osmotic Gradient Ektacytometry)
2.4. Statistical Analysis
3. Results
3.1. Hematological Parameters
3.2. Red Blood Cell Deformability
3.3. Osmotic Gradient Deformability
3.4. Correlation of Parameters
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Variable | Human | Dog | Cat | Pig | Sheep | Mouse | Rat | Rabbit |
---|---|---|---|---|---|---|---|---|
WBC (G/L) | 6.13 ± 0.85 | 11.76 ± 2.33 * | 10.1 ± 4.34 * | 12.93 ± 3.05 * | 145.93 ± 37.13 * | 4.85 ± 0.99 * | 2.57 ± 0.5* | 5.41 ± 0.28 * |
RBC (T/L) | 4.57 ± 0.21 | 8.16 ± 0.51 * | 11.52 ± 0.89 * | 6 ± 0.56 * | 10.41 ± 0.98 * | 8.3 ± 0.41 * | 5.95 ± 0.55 * | 6.02 ± 0.27 * |
Hgb (g/L) | 127.69 ± 10.16 | 185.83 ± 7.73 * | 153.54 ± 14.22 * | 97.44 ± 8.61 * | 115.75 ± 12.23 * | 131.6 ± 6.55 | 108.8 ± 3.61 * | 127.5 ± 4.47 |
Hct (%) | 42.65 ± 1.62 | 55.65 ± 2.75 * | 56.3 ± 5.89 * | 35.23 ± 3.13 * | 43.48 ± 4.19 | 44.6 ± 2.36 * | 36.48 ± 4.23 * | 40.31 ± 1.48 * |
MCV (fL) | 93.4 ± 1.65 | 68.2 ± 1.26 * | 48.85 ± 2.9 * | 59.56 ± 3.11 * | 41.77 ± 0.9 * | 53.72 ± 0.44 * | 61.17 ± 1.96 * | 66.84 ± 1.51 * |
MCH (pg) | 28.04 ± 2.12 | 22.78 ± 0.61 * | 13.33 ± 0.6 * | 16.36 ± 1.75 * | 11.11 ± 0.41 * | 15.87 ± 0.24 * | 18.41 ± 1.68 * | 21.15 ± 0.72 * |
MCHC (g/L) | 309.5 ± 31.2 | 334.25 ± 4.71 | 273.31 ± 10.06 * | 238.56 ± 45.9 * | 266.31 ± 13.59 * | 295.05 ± 4.45 | 301.85 ± 31.29 | 316.25 ± 3.2 |
Plt (G/L) | 260.38 ± 28.66 | 411.67 ± 100.43 * | 396.75 ± 38.51 | 641.88 ± 135 * | 413.5 ± 0.71 * | 880.05 ± 114.38 * | 686.45 ± 118.2 * | 481.88 ± 13.56 * |
Variable | Human | Dog | Cat | Pig | Sheep | Mouse | Rat | Rabbit |
---|---|---|---|---|---|---|---|---|
ΔEI | 0.42 ± 0.02 | 0.43 ± 0.01 | 0.41 ± 0.02 | 0.39 ± 0.02 * | 0.37 ± 0.01 * | 0.47 ± 0.06 * | 0.41 ± 0.04 | 0.41 ± 0.01 |
ΔO | 143.5 ± 4.87 | 147.33 ± 10.29 | 180.2 ± 20.59 * | 181 ± 12.01 * | 179.25 ± 12.24 * | 158.6 ± 13.78 * | 157.8 ± 13.38 * | 163 ± 6.48 * |
ΔEI/ΔO | 0.003 ± 0.00 | 0.003 ± 0.00 | 0.002 ± 0.00 | 0.002 ± 0.00 | 0.002 ± 0.00 | 0.003 ± 0.00 | 0.003 ± 0.00 | 0.003 ± 0.00 |
EI max/EI min (rEI) | 5.39 ± 0.67 | 4.186 ± 0.31 * | 4.96 ± 0.67 | 4.09 ± 0.41 * | 5.61 ± 0.78 | 5.11 ± 0.69 | 4.39 ± 0.74 * | 3.82 ± 0.14 * |
O (EI max)/O min (rO) | 1.9 ± 0.08 | 2.187 ± 0.14 | 1.95 ± 0.09 | 2.01 ± 0.06 | 1.82 ± 0.07 | 1.94 ± 0.09 | 2 ± 0.07 | 2.04 ± 0.03 |
rEI/rO | 2.84 ± 0.41 | 1.93 ± 0.25 * | 2.54 ± 0.32 | 2.03 ± 0.2 * | 3.01 ± 0.45 | 2.64 ± 0.41 | 2.19 ± 0.34 | 1.87 ± 0.07 * |
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Varga, A.; Matrai, A.A.; Barath, B.; Deak, A.; Horvath, L.; Nemeth, N. Interspecies Diversity of Osmotic Gradient Deformability of Red Blood Cells in Human and Seven Vertebrate Animal Species. Cells 2022, 11, 1351. https://doi.org/10.3390/cells11081351
Varga A, Matrai AA, Barath B, Deak A, Horvath L, Nemeth N. Interspecies Diversity of Osmotic Gradient Deformability of Red Blood Cells in Human and Seven Vertebrate Animal Species. Cells. 2022; 11(8):1351. https://doi.org/10.3390/cells11081351
Chicago/Turabian StyleVarga, Adam, Adam Attila Matrai, Barbara Barath, Adam Deak, Laszlo Horvath, and Norbert Nemeth. 2022. "Interspecies Diversity of Osmotic Gradient Deformability of Red Blood Cells in Human and Seven Vertebrate Animal Species" Cells 11, no. 8: 1351. https://doi.org/10.3390/cells11081351