Cerebral Organoids Maintain the Expression of Neural Stem Cell-Associated Glycoepitopes and Extracellular Matrix
1
Department of Cell Morphology and Molecular Neurobiology, Ruhr University Bochum, 44780 Bochum, Germany
2
Institute of Reconstructive Neurobiology, Medical Faculty & University Hospital Bonn, University of Bonn, 53127 Bonn, Germany
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Academic Editor: Luisa Alexandra Meireles Pinto
Cells 2022, 11(5), 760; https://doi.org/10.3390/cells11050760
Received: 23 December 2021
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Revised: 10 February 2022
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Accepted: 14 February 2022
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Published: 22 February 2022
(This article belongs to the Special Issue Frontiers in Neurogenesis)
During development, the nervous system with its highly specialized cell types forms from a pool of relatively uniform stem cells. This orchestrated process requires tight regulation. The extracellular matrix (ECM) is a complex network rich in signaling molecules, and therefore, of interest in this context. Distinct carbohydrate structures, bound to ECM molecules like Tenascin C (TNC), are associated with neural stem/progenitor cells. We have analyzed the expression patterns of the LewisX (LeX) trisaccharide motif and of the sulfation-dependent DSD-1 chondroitin sulfate glycosaminoglycan epitope in human cerebral organoids, a 3D model for early central nervous system (CNS) development, immunohistochemically. In early organoids we observed distinct expression patterns of the glycoepitopes, associated with rosette-like structures that resemble the neural tube in vitro: Terminal LeX motifs, recognized by the monoclonal antibody (mAb) 487LeX, were enriched in the lumen and at the outer border of neural rosettes. In contrast, internal LeX motif repeats detected with mAb 5750LeX were concentrated near the lumen. The DSD-1 epitope, labeled with mAb 473HD, was detectable at rosette borders and in adjacent cells. The epitope expression was maintained in older organoids but appeared more diffuse. The differential glycoepitope expression suggests a specific function in the developing human CNS.
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Keywords:
cerebral organoid; chondroitin sulfate; DSD-1 epitope; extracellular matrix; glycoepitope; LewisX epitope; neural stem cell; PTPRZ1; radial glia; Tenascin C
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MDPI and ACS Style
Roll, L.; Lessmann, K.; Brüstle, O.; Faissner, A. Cerebral Organoids Maintain the Expression of Neural Stem Cell-Associated Glycoepitopes and Extracellular Matrix. Cells 2022, 11, 760. https://doi.org/10.3390/cells11050760
AMA Style
Roll L, Lessmann K, Brüstle O, Faissner A. Cerebral Organoids Maintain the Expression of Neural Stem Cell-Associated Glycoepitopes and Extracellular Matrix. Cells. 2022; 11(5):760. https://doi.org/10.3390/cells11050760
Chicago/Turabian StyleRoll, Lars, Katrin Lessmann, Oliver Brüstle, and Andreas Faissner. 2022. "Cerebral Organoids Maintain the Expression of Neural Stem Cell-Associated Glycoepitopes and Extracellular Matrix" Cells 11, no. 5: 760. https://doi.org/10.3390/cells11050760
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