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Cells
Volume 11
Issue 3
10.3390/cells11030551
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Article

Low Energy Status under Methionine Restriction Is Essentially Independent of Proliferation or Cell Contact Inhibition

by
Corinna Koderer
1,†,
Werner Schmitz
2,†,
Anna Chiara Wünsch
1,
Julia Balint
1,
Mohamed El-Mesery
3,
Julian Manuel Volland
1,
Stefan Hartmann
1,
Christian Linz
1,
Alexander Christian Kübler
1 and
Axel Seher
1,*
1
Department of Oral and Maxillofacial Plastic Surgery, University Hospital Wuerzburg, D-97070 Wuerzburg, Germany
2
Department of Biochemistry and Molecular Biology, Biocenter, D-97074 Wuerzburg, Germany
3
Department of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Cells 2022, 11(3), 551; https://doi.org/10.3390/cells11030551
Submission received: 12 January 2022 / Revised: 2 February 2022 / Accepted: 3 February 2022 / Published: 4 February 2022
(This article belongs to the Section Cellular Metabolism)
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Abstract

Nonlimited proliferation is one of the most striking features of neoplastic cells. The basis of cell division is the sufficient presence of mass (amino acids) and energy (ATP and NADH). A sophisticated intracellular network permanently measures the mass and energy levels. Thus, in vivo restrictions in the form of amino acid, protein, or caloric restrictions strongly affect absolute lifespan and age-associated diseases such as cancer. The induction of permanent low energy metabolism (LEM) is essential in this process. The murine cell line L929 responds to methionine restriction (MetR) for a short time period with LEM at the metabolic level defined by a characteristic fingerprint consisting of the molecules acetoacetate, creatine, spermidine, GSSG, UDP-glucose, pantothenate, and ATP. Here, we used mass spectrometry (LC/MS) to investigate the influence of proliferation and contact inhibition on the energy status of cells. Interestingly, the energy status was essentially independent of proliferation or contact inhibition. LC/MS analyses showed that in full medium, the cells maintain active and energetic metabolism for optional proliferation. In contrast, MetR induced LEM independently of proliferation or contact inhibition. These results are important for cell behaviour under MetR and for the optional application of restrictions in cancer therapy.
Keywords: methionine restriction; caloric restriction; mass spectrometry; LC/MS; liquid chromatography/mass spectrometry; metabolomics; L929; amino acid; proliferation; contact inhibition methionine restriction; caloric restriction; mass spectrometry; LC/MS; liquid chromatography/mass spectrometry; metabolomics; L929; amino acid; proliferation; contact inhibition

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MDPI and ACS Style

Koderer, C.; Schmitz, W.; Wünsch, A.C.; Balint, J.; El-Mesery, M.; Volland, J.M.; Hartmann, S.; Linz, C.; Kübler, A.C.; Seher, A. Low Energy Status under Methionine Restriction Is Essentially Independent of Proliferation or Cell Contact Inhibition. Cells 2022, 11, 551. https://doi.org/10.3390/cells11030551

AMA Style

Koderer C, Schmitz W, Wünsch AC, Balint J, El-Mesery M, Volland JM, Hartmann S, Linz C, Kübler AC, Seher A. Low Energy Status under Methionine Restriction Is Essentially Independent of Proliferation or Cell Contact Inhibition. Cells. 2022; 11(3):551. https://doi.org/10.3390/cells11030551

Chicago/Turabian Style

Koderer, Corinna, Werner Schmitz, Anna Chiara Wünsch, Julia Balint, Mohamed El-Mesery, Julian Manuel Volland, Stefan Hartmann, Christian Linz, Alexander Christian Kübler, and Axel Seher. 2022. "Low Energy Status under Methionine Restriction Is Essentially Independent of Proliferation or Cell Contact Inhibition" Cells 11, no. 3: 551. https://doi.org/10.3390/cells11030551

APA Style

Koderer, C., Schmitz, W., Wünsch, A. C., Balint, J., El-Mesery, M., Volland, J. M., Hartmann, S., Linz, C., Kübler, A. C., & Seher, A. (2022). Low Energy Status under Methionine Restriction Is Essentially Independent of Proliferation or Cell Contact Inhibition. Cells, 11(3), 551. https://doi.org/10.3390/cells11030551

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