The Role of S. cerevisiae Sub1/PC4 in Transcription Elongation Depends on the C-Terminal Region and Is Independent of the ssDNA Binding Domain

Collin, Alejandro; González-Jiménez, Araceli; González-Jiménez, María del Carmen; Alfonso, Manuel J.; Calvo, Olga

doi:10.3390/cells11203320

Open AccessArticle

The Role of S. cerevisiae Sub1/PC4 in Transcription Elongation Depends on the C-Terminal Region and Is Independent of the ssDNA Binding Domain

by

Alejandro Collin

¹,

Araceli González-Jiménez

²,

María del Carmen González-Jiménez

²,

Manuel J. Alfonso

²

and

Olga Calvo

^2,*

¹

Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas-INICSA, CONICET-Universidad Nacional de Córdoba, Haya de la Torre s/n, Pabellón Argentina, 2º piso. Ciudad Universitaria, Cordoba CP5000, Argentina

²

Instituto de Biología Funcional y Genómica (IBFG), CSIC-USAL, C/ Zacarías González, nº2, 37007 Salamanca, Spain

^*

Author to whom correspondence should be addressed.

Cells 2022, 11(20), 3320; https://doi.org/10.3390/cells11203320

Submission received: 7 September 2022 / Revised: 18 October 2022 / Accepted: 19 October 2022 / Published: 21 October 2022

(This article belongs to the Special Issue Control of Gene Expression by Transcription and Co-transcriptional Processes in Cell Homeostasis and Cell Fate Specification)

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Abstract

Saccharomyces cerevisiae Sub1 (ScSub1) has been defined as a transcriptional stimulatory protein due to its homology to the ssDNA binding domain (ssDBD) of human PC4 (hPC4). Recently, PC4/Sub1 orthologues have been elucidated in eukaryotes, prokaryotes, and bacteriophages with functions related to DNA metabolism. Additionally, ScSub1 contains a unique carboxyl–terminal region (CT) of unknown function up to date. Specifically, it has been shown that Sub1 is required for transcription activation, as well as other processes, throughout the transcription cycle. Despite the progress that has been made in understanding the mechanism underlying Sub1′s functions, some questions remain unanswered. As a case in point: whether Sub1’s roles in initiation and elongation are differentially predicated on distinct regions of the protein or how Sub1′s functions are regulated. Here, we uncover some residues that are key for DNA–ScSub1 interaction in vivo, localized in the ssDBD, and required for Sub1 recruitment to promoters. Furthermore, using an array of genetic and molecular techniques, we demonstrate that the CT region is required for transcription elongation by RNA polymerase II (RNAPII). Altogether, our data indicate that Sub1 plays a dual role during transcription—in initiation through the ssDBD and in elongation through the CT region.

Keywords: Sub1; PC4; DNA binding proteins; RNA polymerase II; transcription elongation; Saccharomyces cerevisiae

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MDPI and ACS Style

Collin, A.; González-Jiménez, A.; González-Jiménez, M.d.C.; Alfonso, M.J.; Calvo, O. The Role of S. cerevisiae Sub1/PC4 in Transcription Elongation Depends on the C-Terminal Region and Is Independent of the ssDNA Binding Domain. Cells 2022, 11, 3320. https://doi.org/10.3390/cells11203320

AMA Style

Collin A, González-Jiménez A, González-Jiménez MdC, Alfonso MJ, Calvo O. The Role of S. cerevisiae Sub1/PC4 in Transcription Elongation Depends on the C-Terminal Region and Is Independent of the ssDNA Binding Domain. Cells. 2022; 11(20):3320. https://doi.org/10.3390/cells11203320

Chicago/Turabian Style

Collin, Alejandro, Araceli González-Jiménez, María del Carmen González-Jiménez, Manuel J. Alfonso, and Olga Calvo. 2022. "The Role of S. cerevisiae Sub1/PC4 in Transcription Elongation Depends on the C-Terminal Region and Is Independent of the ssDNA Binding Domain" Cells 11, no. 20: 3320. https://doi.org/10.3390/cells11203320

APA Style

Collin, A., González-Jiménez, A., González-Jiménez, M. d. C., Alfonso, M. J., & Calvo, O. (2022). The Role of S. cerevisiae Sub1/PC4 in Transcription Elongation Depends on the C-Terminal Region and Is Independent of the ssDNA Binding Domain. Cells, 11(20), 3320. https://doi.org/10.3390/cells11203320

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The Role of S. cerevisiae Sub1/PC4 in Transcription Elongation Depends on the C-Terminal Region and Is Independent of the ssDNA Binding Domain

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