Effect of Cell Cycle on Cell Surface Expression of Voltage-Gated Sodium Channels and Na+,K+-ATPase

Edenfield, Samantha; Sims, Abigail M.; Porretta, Constance; Gould, Harry J.; Paul, Dennis

doi:10.3390/cells11203240

Open AccessArticle

Effect of Cell Cycle on Cell Surface Expression of Voltage-Gated Sodium Channels and Na⁺,K⁺-ATPase

by

Samantha Edenfield

¹,

Abigail M. Sims

¹,

Constance Porretta

²,

Harry J. Gould III

^3,4,5,* and

Dennis Paul

^1,3,4,5,6,*

¹

Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA

²

Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA 70112, USA

³

Department of Neurology, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA

⁴

Department of Anesthesiology, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA

⁵

Neuroscience Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA

⁶

Dental and Craniofacial Biology Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, LA 70119, USA

^*

Authors to whom correspondence should be addressed.

Cells 2022, 11(20), 3240; https://doi.org/10.3390/cells11203240

Submission received: 29 June 2022 / Revised: 11 October 2022 / Accepted: 12 October 2022 / Published: 15 October 2022

(This article belongs to the Section Cell Proliferation and Division)

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Abstract

Voltage-gated sodium channels (VGSCs) are the target for many therapies. Variation in membrane potential occurs throughout the cell cycle, yet little attention has been devoted to the role of VGSCs and Na⁺,K⁺-ATPases. We hypothesized that in addition to doubling DNA and cell membrane in anticipation of cell division, there should be a doubling of VGSCs and Na⁺,K⁺-ATPase compared to non-dividing cells. We tested this hypothesis in eight immortalized cell lines by correlating immunocytofluorescent labeling of VGSCs or Na⁺,K⁺-ATPase with propidium iodide or DAPI fluorescence using flow cytometry and imaging. Cell surface expression of VGSCs during phases S through M was double that seen during phases G0–G1. By contrast, Na⁺,K⁺-ATPase expression increased only 1.5-fold. The increases were independent of baseline expression of channels or pumps. The variation in VGSC and Na⁺,K⁺-ATPase expression has implications for both our understanding of sodium’s role in controlling the cell cycle and variability of treatments targeted at these components of the Na⁺ handling system.

Keywords: voltage-gated sodium channels; VGSCs; Na⁺,K⁺-ATPase; sodium pumps; cell cycle

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MDPI and ACS Style

Edenfield, S.; Sims, A.M.; Porretta, C.; Gould, H.J., III; Paul, D. Effect of Cell Cycle on Cell Surface Expression of Voltage-Gated Sodium Channels and Na⁺,K⁺-ATPase. Cells 2022, 11, 3240. https://doi.org/10.3390/cells11203240

AMA Style

Edenfield S, Sims AM, Porretta C, Gould HJ III, Paul D. Effect of Cell Cycle on Cell Surface Expression of Voltage-Gated Sodium Channels and Na⁺,K⁺-ATPase. Cells. 2022; 11(20):3240. https://doi.org/10.3390/cells11203240

Chicago/Turabian Style

Edenfield, Samantha, Abigail M. Sims, Constance Porretta, Harry J. Gould, III, and Dennis Paul. 2022. "Effect of Cell Cycle on Cell Surface Expression of Voltage-Gated Sodium Channels and Na⁺,K⁺-ATPase" Cells 11, no. 20: 3240. https://doi.org/10.3390/cells11203240

APA Style

Edenfield, S., Sims, A. M., Porretta, C., Gould, H. J., III, & Paul, D. (2022). Effect of Cell Cycle on Cell Surface Expression of Voltage-Gated Sodium Channels and Na⁺,K⁺-ATPase. Cells, 11(20), 3240. https://doi.org/10.3390/cells11203240

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Effect of Cell Cycle on Cell Surface Expression of Voltage-Gated Sodium Channels and Na⁺,K⁺-ATPase

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