The Properties of Proinflammatory Ly6Chi Monocytes Are Differentially Shaped by Parasitic and Bacterial Liver Infections
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. Infection of Mice with E. histolytica and L. monocytogenes
2.3. Immunohistochemistry
2.4. Cytokine Measurement
2.5. Detection of Reactive Oxygen Species
2.6. RNA Sequencing and Data Analysis
2.7. Isolation of Immune Cells and Flow Cytometry
2.8. Quantitative RT-PCR
2.9. Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP)
2.10. Statistical Analysis
3. Results
3.1. Different Recruitment and Localization of Ly6ChiCD11b+ Monocytes in the Liver following Infection with E. histolytica or L. monocytogenes
3.2. Monocytes from Both Infection Models Show Significant Differences in Gene Expression
3.3. Ly6Chi Monocytes from L. monocytogenes-Infected Mice Have an Activated Phenotype and Lower M2 Polarization at the Transcriptional Level Than Ly6Chi Monocytes from E. histolytica-Infected Mice
3.4. Surface Marker Expression Implies Pathogen-Dependent Subsets of Proinflammatory Monocytes
3.5. CD38+Ly6Chi Monocytes Produce ROS and Contribute to Monocyte-Dependent Immunopathology during Hepatic Amebiasis
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hoenow, S.; Yan, K.; Noll, J.; Groneberg, M.; Casar, C.; Lory, N.C.; Vogelsang, M.; Hansen, C.; Wolf, V.; Fehling, H.; et al. The Properties of Proinflammatory Ly6Chi Monocytes Are Differentially Shaped by Parasitic and Bacterial Liver Infections. Cells 2022, 11, 2539. https://doi.org/10.3390/cells11162539
Hoenow S, Yan K, Noll J, Groneberg M, Casar C, Lory NC, Vogelsang M, Hansen C, Wolf V, Fehling H, et al. The Properties of Proinflammatory Ly6Chi Monocytes Are Differentially Shaped by Parasitic and Bacterial Liver Infections. Cells. 2022; 11(16):2539. https://doi.org/10.3390/cells11162539
Chicago/Turabian StyleHoenow, Stefan, Karsten Yan, Jill Noll, Marie Groneberg, Christian Casar, Niels Christian Lory, Malte Vogelsang, Charlotte Hansen, Vincent Wolf, Helena Fehling, and et al. 2022. "The Properties of Proinflammatory Ly6Chi Monocytes Are Differentially Shaped by Parasitic and Bacterial Liver Infections" Cells 11, no. 16: 2539. https://doi.org/10.3390/cells11162539