A Novel Cre/lox71-Based System for Inducible Expression of Recombinant Proteins and Genome Editing
Abstract
:1. Introduction
2. Materials and Methods
2.1. Genetic Constructs Encoding Cre-VP64 Transactivator and lox71-Based Promote
2.2. Cell Culture and Transfection
2.3. Inducible Transgene Expression Using Cre-VP64/lox71 System
2.4. Inducible Genome Editing Using Cre-VP64/lox71 System
2.5. Statistical Analysis
3. Results
3.1. Characterization of Cre-VP64/lox71 System
3.2. Cre-VP64/lox71 System Provides a High Level of Transgene Expression
3.3. Inducible Genome Editing Using Cre-VP64/lox71 System
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Karagyaur, M.; Dyikanov, D.; Tyurin-Kuzmin, P.; Dzhauari, S.; Skryabina, M.; Vigovskiy, M.; Primak, A.; Kalinina, N.; Tkachuk, V. A Novel Cre/lox71-Based System for Inducible Expression of Recombinant Proteins and Genome Editing. Cells 2022, 11, 2141. https://doi.org/10.3390/cells11142141
Karagyaur M, Dyikanov D, Tyurin-Kuzmin P, Dzhauari S, Skryabina M, Vigovskiy M, Primak A, Kalinina N, Tkachuk V. A Novel Cre/lox71-Based System for Inducible Expression of Recombinant Proteins and Genome Editing. Cells. 2022; 11(14):2141. https://doi.org/10.3390/cells11142141
Chicago/Turabian StyleKaragyaur, Maxim, Daniyar Dyikanov, Pyotr Tyurin-Kuzmin, Stalik Dzhauari, Mariya Skryabina, Maksim Vigovskiy, Alexandra Primak, Natalia Kalinina, and Vsevolod Tkachuk. 2022. "A Novel Cre/lox71-Based System for Inducible Expression of Recombinant Proteins and Genome Editing" Cells 11, no. 14: 2141. https://doi.org/10.3390/cells11142141