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Article

Combination of Carbonate Hydroxyapatite and Stem Cells from Human Deciduous Teeth Promotes Bone Regeneration by Enhancing BMP-2, VEGF and CD31 Expression in Immunodeficient Mice

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Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical and Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan
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Department of Oral Biology and Engineering, Division of Oral Health Sciences, Institute of Biomedical and Health Sciences, Hiroshima University Graduate School of Biomedical and Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Li Xiao and Takeo Tsutsui
Cells 2022, 11(12), 1914; https://doi.org/10.3390/cells11121914
Received: 18 May 2022 / Revised: 6 June 2022 / Accepted: 10 June 2022 / Published: 13 June 2022
(This article belongs to the Special Issue Recent Advances in Regenerative Dentistry)
The objective of this study was to clarify the efficiency of a combination of stem cells from human deciduous teeth and carbonate apatite in bone regeneration of calvarial defects. Immunodeficient mice (n = 5 for each group/4 groups) with artificial calvarial bone defects (5 mm in diameter) were developed, and stem cells from human deciduous teeth (SHEDs) and carbonate hydroxyapatite (CAP) granules were transplanted with an atelocollagen sponge as a scaffold. A 3D analysis using microcomputed tomography, and 12 weeks after transplantation, histological and immunohistochemical evaluations of markers of bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF), and cluster of differentiation (CD) 31 were performed. In the 3D analysis, regenerated bone formation was observed in SHEDs and CAP, with the combination of SHEDs and CAP showing significantly greater bone regeneration than that in the other groups. Histological and immunohistochemical evaluations showed that combining SHEDs and CAP enhanced the expression of BMP-2, VEGF, and CD31, and promoted bone regeneration. This study demonstrates that the combination of SHEDs and CAP transplantation may be a promising tool for bone regeneration in alveolar defects. View Full-Text
Keywords: bone regeneration; stem cell from human deciduous teeth; carbonate apatite; bone grafting; bone morphogenetic protein-2; vascular endothelial growth factor bone regeneration; stem cell from human deciduous teeth; carbonate apatite; bone grafting; bone morphogenetic protein-2; vascular endothelial growth factor
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MDPI and ACS Style

Putranti, N.A.R.; Kunimatsu, R.; Rikitake, K.; Hiraki, T.; Nakajima, K.; Abe, T.; Tsuka, Y.; Sakata, S.; Nakatani, A.; Nikawa, H.; Tanimoto, K. Combination of Carbonate Hydroxyapatite and Stem Cells from Human Deciduous Teeth Promotes Bone Regeneration by Enhancing BMP-2, VEGF and CD31 Expression in Immunodeficient Mice. Cells 2022, 11, 1914. https://doi.org/10.3390/cells11121914

AMA Style

Putranti NAR, Kunimatsu R, Rikitake K, Hiraki T, Nakajima K, Abe T, Tsuka Y, Sakata S, Nakatani A, Nikawa H, Tanimoto K. Combination of Carbonate Hydroxyapatite and Stem Cells from Human Deciduous Teeth Promotes Bone Regeneration by Enhancing BMP-2, VEGF and CD31 Expression in Immunodeficient Mice. Cells. 2022; 11(12):1914. https://doi.org/10.3390/cells11121914

Chicago/Turabian Style

Putranti, Nurul Aisyah Rizky, Ryo Kunimatsu, Kodai Rikitake, Tomoka Hiraki, Kengo Nakajima, Takaharu Abe, Yuji Tsuka, Shuzo Sakata, Ayaka Nakatani, Hiroki Nikawa, and Kotaro Tanimoto. 2022. "Combination of Carbonate Hydroxyapatite and Stem Cells from Human Deciduous Teeth Promotes Bone Regeneration by Enhancing BMP-2, VEGF and CD31 Expression in Immunodeficient Mice" Cells 11, no. 12: 1914. https://doi.org/10.3390/cells11121914

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