Renal Ischemia Induces Epigenetic Changes in Apoptotic, Proteolytic, and Mitochondrial Genes in Swine Scattered Tubular-like Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Design
2.2. STC Isolation and Characterization
2.3. MeDIP- and hMeDIP-seq
2.4. mRNA-seq and Integrated (MeDIP- and hMeDIP-seq/mRNA-seq) Analysis
2.5. Validation of MeDIP- and hMeDIP-seq/mRNA-seq Analysis
2.6. Functional Studies
2.7. Statistical Analysis
3. Results
3.1. STC Characterization
3.2. RAS Alters 5mC and 5hmC Levels in Swine STCs
3.3. RAS Induces Changes in Gene Expression in Swine STCs
3.4. RAS Modulates Apoptosis, Proteolysis, and Mitochondrial Function in Swine STCs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameter | Normal | RAS |
---|---|---|
Body Weight (Kg) | 51.5 ± 0.5 | 54.7 ± 5.5 |
Degree of stenosis (%) | 0 | 86.7 ± 11.6 * |
Systolic blood pressure (mmHg) | 96.7 ± 1.5 | 158.3 ± 21.2 * |
Diastolic blood pressure (mmHg) | 71.7 ± 3.8 | 116 ± 18.5 * |
Mean arterial pressure (mmHg) | 80.5 ± 2.8 | 131.5 ± 19.2 * |
Serum creatinine (mg/dL) | 1.1 ± 0.0 | 1.9 ± 0.1 * |
Cortical volume (mL) | 104.3 ± 4.6 | 59.2 ± 25.8 * |
Medullary volume (mL) | 20.1 ± 1.0 | 20.6 ± 1.6 |
Cortical perfusion (mL/min/mL tissue) | 5.6 ± 0.2 | 2.4 ± 0.5 * |
Medullary perfusion (mL/min/mL tissue) | 2.7 ± 0.3 | 2.5 ± 0.3 |
RBF (mL/min) | 585.2 ± 65.8 | 343.9 ± 69.1 * |
GFR (mL/min) | 93.6 ± 6.4 | 56.1 ± 9.1 * |
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Rajagopalan, K.S.; Glasstetter, L.M.; Zhu, X.-Y.; Thaler, R.; Tang, H.; Jordan, K.L.; Saadiq, I.M.; Herrmann, S.M.; Chade, A.R.; Irazabal, M.V.; et al. Renal Ischemia Induces Epigenetic Changes in Apoptotic, Proteolytic, and Mitochondrial Genes in Swine Scattered Tubular-like Cells. Cells 2022, 11, 1803. https://doi.org/10.3390/cells11111803
Rajagopalan KS, Glasstetter LM, Zhu X-Y, Thaler R, Tang H, Jordan KL, Saadiq IM, Herrmann SM, Chade AR, Irazabal MV, et al. Renal Ischemia Induces Epigenetic Changes in Apoptotic, Proteolytic, and Mitochondrial Genes in Swine Scattered Tubular-like Cells. Cells. 2022; 11(11):1803. https://doi.org/10.3390/cells11111803
Chicago/Turabian StyleRajagopalan, Kamalnath S., Logan M. Glasstetter, Xiang-Yang Zhu, Roman Thaler, Hui Tang, Kyra L. Jordan, Ishran M. Saadiq, Sandra M. Herrmann, Alejandro R. Chade, Maria V. Irazabal, and et al. 2022. "Renal Ischemia Induces Epigenetic Changes in Apoptotic, Proteolytic, and Mitochondrial Genes in Swine Scattered Tubular-like Cells" Cells 11, no. 11: 1803. https://doi.org/10.3390/cells11111803