Porin 1 Modulates Autophagy in Yeast

Chair of Biochemistry and Molecular Medicine, Center for Biomedical Education and Research (ZBAF), University of Witten/Herdecke (UW/H), Stockumer Str. 10, 58453 Witten, Germany

Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 10691 Stockholm, Sweden

Kent Fungal Group, School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK

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Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany

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Institute of Molecular Biosciences, NAWI Graz, University of Graz, 8010 Graz, Austria

Authors to whom correspondence should be addressed.

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Current affiliation: Nuvisan ICB GmbH, 13353 Berlin, Germany.

Cells 2021, 10(9), 2416; https://doi.org/10.3390/cells10092416

Received: 20 August 2021 / Revised: 7 September 2021 / Accepted: 9 September 2021 / Published: 14 September 2021

(This article belongs to the Section Autophagy)

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Abstract

Autophagy is a cellular recycling program which efficiently reduces the cellular burden of ageing. Autophagy is characterised by nucleation of isolation membranes, which grow in size and further expand to form autophagosomes, engulfing cellular material to be degraded by fusion with lysosomes (vacuole in yeast). Autophagosomal membranes do not bud from a single cell organelle, but are generated de novo. Several lipid sources for autophagosomal membranes have been identified, but the whole process of their generation is complex and not entirely understood. In this study, we investigated how the mitochondrial outer membrane protein porin 1 (Por1), the yeast orthologue of mammalian voltage-dependent anion channel (VDAC), affects autophagy in yeast. We show that POR1 deficiency reduces the autophagic capacity and leads to changes in vacuole and lipid homeostasis. We further investigated whether limited phosphatidylethanolamine (PE) availability in por1∆ was causative for reduced autophagy by overexpression of the PE-generating phosphatidylserine decarboxylase 1 (Psd1). Altogether, our results show that POR1 deficiency is associated with reduced autophagy, which can be circumvented by additional PSD1 overexpression. This suggests a role for Por1 in Psd1-mediated autophagy regulation. View Full-Text

Keywords: autophagy; voltage dependent anion channel (VDAC); phosphatidylethanolamine; phosphatidylserine decarboxylase

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MDPI and ACS Style

Broeskamp, F.; Edrich, E.S.M.; Knittelfelder, O.; Neuhaus, L.; Meyer, T.; Heyden, J.; Habernig, L.; Kreppel, F.; Gourlay, C.W.; Rockenfeller, P. Porin 1 Modulates Autophagy in Yeast. Cells 2021, 10, 2416. https://doi.org/10.3390/cells10092416

AMA Style

Broeskamp F, Edrich ESM, Knittelfelder O, Neuhaus L, Meyer T, Heyden J, Habernig L, Kreppel F, Gourlay CW, Rockenfeller P. Porin 1 Modulates Autophagy in Yeast. Cells. 2021; 10(9):2416. https://doi.org/10.3390/cells10092416

Chicago/Turabian Style

Broeskamp, Filomena, Elizabeth S.M. Edrich, Oskar Knittelfelder, Lisa Neuhaus, Thorsten Meyer, Jonas Heyden, Lukas Habernig, Florian Kreppel, Campbell W. Gourlay, and Patrick Rockenfeller. 2021. "Porin 1 Modulates Autophagy in Yeast" Cells 10, no. 9: 2416. https://doi.org/10.3390/cells10092416

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