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Article

Interferon-Induced HERC5 Inhibits Ebola Virus Particle Production and Is Antagonized by Ebola Glycoprotein

1
Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, Western University, Dental Sciences Building Room 3007, London, ON N6A 5C1, Canada
2
Department of Biochemistry, Microbiology, and Immunology, Ottawa Institute of Systems Biology, University of Ottawa, Roger-Guindon Hall Room 4214, Ottawa, ON K1H 8M5 , Canada
3
Friedrich-Loeffler-Institut, Institute of Molecular Virology and Cell Biology, Südufer 10, 17493 Greifswald—Insel Riems, Germany
*
Author to whom correspondence should be addressed.
Academic Editors: Reinhild Prange and Alexander E. Kalyuzhny
Cells 2021, 10(9), 2399; https://doi.org/10.3390/cells10092399
Received: 29 April 2021 / Revised: 11 August 2021 / Accepted: 31 August 2021 / Published: 13 September 2021
(This article belongs to the Special Issue Virus — Host Cell Interactions)
Survival following Ebola virus (EBOV) infection correlates with the ability to mount an early and robust interferon (IFN) response. The host IFN-induced proteins that contribute to controlling EBOV replication are not fully known. Among the top genes with the strongest early increases in expression after infection in vivo is IFN-induced HERC5. Using a transcription- and replication-competent VLP system, we showed that HERC5 inhibits EBOV virus-like particle (VLP) replication by depleting EBOV mRNAs. The HERC5 RCC1-like domain was necessary and sufficient for this inhibition and did not require zinc finger antiviral protein (ZAP). Moreover, we showed that EBOV (Zaire) glycoprotein (GP) but not Marburg virus GP antagonized HERC5 early during infection. Our data identify a novel ‘protagonist–antagonistic’ relationship between HERC5 and GP in the early stages of EBOV infection that could be exploited for the development of novel antiviral therapeutics. View Full-Text
Keywords: Ebola virus; Marburg virus; HERC5; antiviral; interferon Ebola virus; Marburg virus; HERC5; antiviral; interferon
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MDPI and ACS Style

Paparisto, E.; Hunt, N.R.; Labach, D.S.; Coleman, M.D.; Di Gravio, E.J.; Dodge, M.J.; Friesen, N.J.; Côté, M.; Müller, A.; Hoenen, T.; Barr, S.D. Interferon-Induced HERC5 Inhibits Ebola Virus Particle Production and Is Antagonized by Ebola Glycoprotein. Cells 2021, 10, 2399. https://doi.org/10.3390/cells10092399

AMA Style

Paparisto E, Hunt NR, Labach DS, Coleman MD, Di Gravio EJ, Dodge MJ, Friesen NJ, Côté M, Müller A, Hoenen T, Barr SD. Interferon-Induced HERC5 Inhibits Ebola Virus Particle Production and Is Antagonized by Ebola Glycoprotein. Cells. 2021; 10(9):2399. https://doi.org/10.3390/cells10092399

Chicago/Turabian Style

Paparisto, Ermela, Nina R. Hunt, Daniel S. Labach, Macon D. Coleman, Eric J. Di Gravio, Mackenzie J. Dodge, Nicole J. Friesen, Marceline Côté, Andreas Müller, Thomas Hoenen, and Stephen D. Barr 2021. "Interferon-Induced HERC5 Inhibits Ebola Virus Particle Production and Is Antagonized by Ebola Glycoprotein" Cells 10, no. 9: 2399. https://doi.org/10.3390/cells10092399

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