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Article

Novel, Fully Characterised Bovine Taste Bud Cells of Fungiform Papillae

Aston Pharmacy School, Aston University, Birmingham B4 7ET, UK
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Author to whom correspondence should be addressed.
Academic Editor: Tobias Cantz
Cells 2021, 10(9), 2285; https://doi.org/10.3390/cells10092285
Received: 27 June 2021 / Revised: 23 August 2021 / Accepted: 28 August 2021 / Published: 2 September 2021
(This article belongs to the Section Cell Methods)
Current understanding of functional characteristics and biochemical pathways in taste bud cells have been hindered due the lack of long-term cultured cells. To address this, we developed a holistic approach to fully characterise long term cultured bovine taste bud cells (BTBCs). Initially, cultured BTBCs were characterised using RT-PCR gene expression profiling, immunocytochemistry, flowcytometry and calcium imaging, that confirmed the cells were mature TBCs that express taste receptor genes, taste specific protein markers and capable of responding to taste stimuli, i.e., denatonium (2 mM) and quinine (462.30 μM). Gene expression analysis of forty-two genes implicated in taste transduction pathway (map04742) using custom-made RT-qPCR array revealed high and low expressed genes in BTBCs. Preliminary datamining and bioinformatics demonstrated that the bovine α-gustducin, gustatory G-protein, have higher sequence similarity to the human orthologue compared to rodents. Therefore, results from this work will replace animal experimentation and provide surrogate cell-based throughput system to study human taste transduction. View Full-Text
Keywords: taste bud cells; taste cell characterization; PCR taste bud cells; taste cell characterization; PCR
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MDPI and ACS Style

Ftuwi, H.; Parri, R.; Mohammed, A.R. Novel, Fully Characterised Bovine Taste Bud Cells of Fungiform Papillae. Cells 2021, 10, 2285. https://doi.org/10.3390/cells10092285

AMA Style

Ftuwi H, Parri R, Mohammed AR. Novel, Fully Characterised Bovine Taste Bud Cells of Fungiform Papillae. Cells. 2021; 10(9):2285. https://doi.org/10.3390/cells10092285

Chicago/Turabian Style

Ftuwi, Habtom, Rheinallt Parri, and Afzal R. Mohammed 2021. "Novel, Fully Characterised Bovine Taste Bud Cells of Fungiform Papillae" Cells 10, no. 9: 2285. https://doi.org/10.3390/cells10092285

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