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Article

Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study

1
School of Biosciences, University of Kent, Canterbury CT2 7NH, UK
2
L’Alliance Boviteq Inc., Saint-Hyacinthe, QC J2T 5H1, Canada
3
School of Biosciences, University of Nottingham, Nottingham LE12 5RD, UK
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Geert J.P.L. Kops
Cells 2021, 10(9), 2284; https://doi.org/10.3390/cells10092284
Received: 9 August 2021 / Revised: 29 August 2021 / Accepted: 30 August 2021 / Published: 2 September 2021
(This article belongs to the Special Issue Non-human Chromosome Analysis)
Approximately one million in vitro produced (IVP) cattle embryos are transferred worldwide each year as a way to improve the rates of genetic gain. The most advanced programmes also apply genomic selection at the embryonic stage by SNP genotyping and the calculation of genomic estimated breeding values (GEBVs). However, a high proportion of cattle embryos fail to establish a pregnancy. Here, we demonstrate that further interrogation of the SNP data collected for GEBVs can effectively remove aneuploid embryos from the pool, improving live births per embryo transfer (ET). Using three preimplantation genetic testing for aneuploidy (PGT-A) approaches, we assessed 1713 cattle blastocysts in a blind, retrospective analysis. Our findings indicate aneuploid embryos have a 5.8% chance of establishing a pregnancy and a 5.0% chance of given rise to a live birth. This compares to 59.6% and 46.7% for euploid embryos (p < 0.0001). PGT-A improved overall pregnancy and live birth rates by 7.5% and 5.8%, respectively (p < 0.0001). More detailed analyses revealed donor, chromosome, stage, grade, and sex-specific rates of error. Notably, we discovered a significantly higher incidence of aneuploidy in XY embryos and, as in humans, detected a preponderance of maternal meiosis I errors. Our data strongly support the use of PGT-A in cattle IVP programmes. View Full-Text
Keywords: cattle; breeding; single nucleotide polymorphism (SNP); mosaicism cattle; breeding; single nucleotide polymorphism (SNP); mosaicism
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MDPI and ACS Style

Silvestri, G.; Canedo-Ribeiro, C.; Serrano-Albal, M.; Labrecque, R.; Blondin, P.; Larmer, S.G.; Marras, G.; Tutt, D.A.R.; Handyside, A.H.; Farré, M.; Sinclair, K.D.; Griffin, D.K. Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study. Cells 2021, 10, 2284. https://doi.org/10.3390/cells10092284

AMA Style

Silvestri G, Canedo-Ribeiro C, Serrano-Albal M, Labrecque R, Blondin P, Larmer SG, Marras G, Tutt DAR, Handyside AH, Farré M, Sinclair KD, Griffin DK. Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study. Cells. 2021; 10(9):2284. https://doi.org/10.3390/cells10092284

Chicago/Turabian Style

Silvestri, Giuseppe, Carla Canedo-Ribeiro, María Serrano-Albal, Remi Labrecque, Patrick Blondin, Steven G. Larmer, Gabriele Marras, Desmond A.R. Tutt, Alan H. Handyside, Marta Farré, Kevin D. Sinclair, and Darren K. Griffin 2021. "Preimplantation Genetic Testing for Aneuploidy Improves Live Birth Rates with In Vitro Produced Bovine Embryos: A Blind Retrospective Study" Cells 10, no. 9: 2284. https://doi.org/10.3390/cells10092284

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