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Article

Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System

1
Futuristic Animal Resource and Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang 28116, Korea
2
Department of Functional Genomics, KRIBB School of Bioscience, Korea University of Science and Technology, Daejeon 34113, Korea
3
Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea
4
College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju 28644, Korea
5
Research Institute, eBiogen Inc., Seoul 04785, Korea
6
National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Ochang 28116, Korea
*
Authors to whom correspondence should be addressed.
Those authors contributed equally to this work.
Academic Editor: Hiroshi Yagi
Cells 2021, 10(8), 2036; https://doi.org/10.3390/cells10082036
Received: 9 July 2021 / Revised: 30 July 2021 / Accepted: 5 August 2021 / Published: 9 August 2021
(This article belongs to the Special Issue Current Progress in Organ Regeneration: Cells, Organoids and Organs)
Vascularization of tissues, organoids and organ-on-chip models has been attempted using endothelial cells. However, the cultured endothelial cells lack the capacity to interact with other somatic cell types, which is distinct from developing vascular cells in vivo. Recently, it was demonstrated that blood vessel organoids (BVOs) recreate the structure and functions of developing human blood vessels. However, the tissue-specific adaptability of BVOs had not been assessed in somatic tissues. Herein, we investigated whether BVOs infiltrate human cerebral organoids and form a blood–brain barrier. As a result, vascular cells arising from BVOs penetrated the cerebral organoids and developed a vessel-like architecture composed of CD31+ endothelial tubes coated with SMA+ or PDGFR+ mural cells. Molecular markers of the blood-brain barrier were detected in the vascularized cerebral organoids. We revealed that BVOs can form neural-specific blood-vessel networks that can be maintained for over 50 days. View Full-Text
Keywords: vascularization; blood vessel organoid; cerebral organoid; blood-brain barrier; vessel structure; organoids; blood vessel formation; brain organoid vascularization; blood vessel organoid; cerebral organoid; blood-brain barrier; vessel structure; organoids; blood vessel formation; brain organoid
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MDPI and ACS Style

Ahn, Y.; An, J.-H.; Yang, H.-J.; Lee, D.G.; Kim, J.; Koh, H.; Park, Y.-H.; Song, B.-S.; Sim, B.-W.; Lee, H.J.; Lee, J.-H.; Kim, S.-U. Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System. Cells 2021, 10, 2036. https://doi.org/10.3390/cells10082036

AMA Style

Ahn Y, An J-H, Yang H-J, Lee DG, Kim J, Koh H, Park Y-H, Song B-S, Sim B-W, Lee HJ, Lee J-H, Kim S-U. Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System. Cells. 2021; 10(8):2036. https://doi.org/10.3390/cells10082036

Chicago/Turabian Style

Ahn, Yujin, Ju-Hyun An, Hae-Jun Yang, Dong G. Lee, Jieun Kim, Hyebin Koh, Young-Ho Park, Bong-Seok Song, Bo-Woong Sim, Hong J. Lee, Jong-Hee Lee, and Sun-Uk Kim. 2021. "Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System" Cells 10, no. 8: 2036. https://doi.org/10.3390/cells10082036

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