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Article

β-(1→4)-Mannobiose Acts as an Immunostimulatory Molecule in Murine Dendritic Cells by Binding the TLR4/MD-2 Complex

1
Laboratory of Food and Biomolecular Science, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan
2
VPr1 Research Group: “Molecular Allergology”, Paul-Ehrlich-Institut, 63225 Langen, Germany
3
Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan
*
Authors to whom correspondence should be addressed.
Equal contribution.
Academic Editor: Alessandro Poggi
Cells 2021, 10(7), 1774; https://doi.org/10.3390/cells10071774
Received: 26 May 2021 / Revised: 5 July 2021 / Accepted: 8 July 2021 / Published: 14 July 2021
(This article belongs to the Collection Feature Papers in Cellular Immunology)
Some β-mannans, including those in coffee bean and soy, contain a mannose backbone with β-(1→4) bonds. Such mannooligosaccharides could have immunological functions involving direct interaction with immune cells, in addition to acting as prebiotics. This study aimed at assessing the immunological function of mannooligosaccharides with β-(1→4) bond, and elucidating their mechanism of action using bone marrow-derived murine dendritic cells (BMDCs). When BMDCs were stimulated with the mannooligosaccharides, only β-Man-(1→4)-Man significantly induced production of cytokines that included IL-6, IL-10, TNF-α, and IFN-β, and enhanced CD4+ T-cell stimulatory capacity. Use of putative receptor inhibitors revealed the binding of β-Man-(1→4)-Man to TLR4/MD2 complex and involvement with the complement C3a receptor (C3aR) for BMDC activation. Interestingly, β-Man-(1→4)-Man prolonged the production of pro-inflammatory cytokines (IL-6 and TNF-α), but not of the IL-10 anti-inflammatory cytokine during extended culture of BMDCs, associated with high glucose consumption. The results suggest that β-Man-(1→4)-Man is an immunostimulatory molecule, and that the promotion of glycolysis could be involved in the production of pro-inflammatory cytokine in β-Man-(1→4)-Man-stimulated BMDCs. This study could contribute to development of immune-boosting functional foods and a novel vaccine adjuvant. View Full-Text
Keywords: mannobiose; dendritic cells; immunostimulation mannobiose; dendritic cells; immunostimulation
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MDPI and ACS Style

Cheng, T.-Y.; Lin, Y.-J.; Saburi, W.; Vieths, S.; Scheurer, S.; Schülke, S.; Toda, M. β-(1→4)-Mannobiose Acts as an Immunostimulatory Molecule in Murine Dendritic Cells by Binding the TLR4/MD-2 Complex. Cells 2021, 10, 1774. https://doi.org/10.3390/cells10071774

AMA Style

Cheng T-Y, Lin Y-J, Saburi W, Vieths S, Scheurer S, Schülke S, Toda M. β-(1→4)-Mannobiose Acts as an Immunostimulatory Molecule in Murine Dendritic Cells by Binding the TLR4/MD-2 Complex. Cells. 2021; 10(7):1774. https://doi.org/10.3390/cells10071774

Chicago/Turabian Style

Cheng, Ting-Yu, Yen-Ju Lin, Wataru Saburi, Stefan Vieths, Stephan Scheurer, Stefan Schülke, and Masako Toda. 2021. "β-(1→4)-Mannobiose Acts as an Immunostimulatory Molecule in Murine Dendritic Cells by Binding the TLR4/MD-2 Complex" Cells 10, no. 7: 1774. https://doi.org/10.3390/cells10071774

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