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New Stable Cell Lines Derived from the Proximal and Distal Intestine of Rainbow Trout (Oncorhynchus mykiss) Retain Several Properties Observed In Vivo

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Department of Agricultural and Environmental Sciences, University of Milan, 20133 Milano, Italy
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Department of Health, Animal Science and Food Safety, University of Milan, 20133 Milano, Italy
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Department of Environmental Toxicology, Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600 Dübendorf, Switzerland
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Department of Environmental Systems Science, ETH Zürich, 8092 Zürich, Switzerland
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School of Architecture, Civil and Environmental Engineering, EPFL Lausanne, 1015 Lausanne, Switzerland
*
Author to whom correspondence should be addressed.
Academic Editor: Iban Seiliez
Cells 2021, 10(6), 1555; https://doi.org/10.3390/cells10061555
Received: 18 May 2021 / Revised: 15 June 2021 / Accepted: 17 June 2021 / Published: 19 June 2021
We derived two novel cell lines from rainbow trout (RT) proximal (RTpi-MI) and distal intestine (RTdi-MI) and compared them with the previously established continuous cell line RTgutGC. Intestinal stem cells, differentiating and differentiated epithelial cells, and connective cells were found in all cell lines. The cell lines formed a polarized barrier, which was not permeable to large molecules and absorbed proline and glucose. High seeding density induced their differentiation into more mature phenotypes, as indicated by the downregulation of intestinal stem cell-related genes (i.e., sox9, hopx and lgr5), whereas alkaline phosphatase activity was upregulated. Other enterocyte markers (i.e., sglt1 and pept1), however, were not regulated as expected. In all cell lines, the presence of a mixed population of epithelial and stromal cells was characterized for the first time. The expression by the stromal component of lgr5, a stem cell niche regulatory molecule, may explain why these lines proliferate stably in vitro. Although most parameters were conserved among the three cell lines, some significant differences were observed, suggesting that characteristics typical of each tract are partly conserved in vitro as well. View Full-Text
Keywords: fish; intestinal cell lines; intestinal stem cells; enterocyte differentiation markers; intestinal barrier in vitro; cell differentiation; nutrition fish; intestinal cell lines; intestinal stem cells; enterocyte differentiation markers; intestinal barrier in vitro; cell differentiation; nutrition
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MDPI and ACS Style

Pasquariello, R.; Verdile, N.; Pavlovic, R.; Panseri, S.; Schirmer, K.; Brevini, T.A.L.; Gandolfi, F. New Stable Cell Lines Derived from the Proximal and Distal Intestine of Rainbow Trout (Oncorhynchus mykiss) Retain Several Properties Observed In Vivo. Cells 2021, 10, 1555. https://doi.org/10.3390/cells10061555

AMA Style

Pasquariello R, Verdile N, Pavlovic R, Panseri S, Schirmer K, Brevini TAL, Gandolfi F. New Stable Cell Lines Derived from the Proximal and Distal Intestine of Rainbow Trout (Oncorhynchus mykiss) Retain Several Properties Observed In Vivo. Cells. 2021; 10(6):1555. https://doi.org/10.3390/cells10061555

Chicago/Turabian Style

Pasquariello, Rolando, Nicole Verdile, Radmila Pavlovic, Sara Panseri, Kristin Schirmer, Tiziana A.L. Brevini, and Fulvio Gandolfi. 2021. "New Stable Cell Lines Derived from the Proximal and Distal Intestine of Rainbow Trout (Oncorhynchus mykiss) Retain Several Properties Observed In Vivo" Cells 10, no. 6: 1555. https://doi.org/10.3390/cells10061555

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