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Article

Functional Characterization of the Photosynthetic Machinery in Smicronix Galls on the Parasitic Plant Cuscuta campestris by JIP-Test

1
Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, Bulgaria
2
AgroBioInstitute, Agricultural Academy, 8 Dragan Tsankov Blvd., 1164 Sofia, Bulgaria
3
Institute of Biology, Warsaw University of Life Sciences–SGGW, 02–787 Warsaw, Poland
4
Institute of Technology and Life Sciences-National Research Institute, Falenty, Al. Hrabska 3, 05-090 Raszyn, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Tatsuya Tomo
Cells 2021, 10(6), 1399; https://doi.org/10.3390/cells10061399
Received: 20 April 2021 / Revised: 31 May 2021 / Accepted: 3 June 2021 / Published: 5 June 2021
Members of the genus Cuscuta are generally considered to be non-photosynthetic, stem-holoparasitic flowering plants. Under certain circumstances, at least some members of the genus are capable of limited photosynthesis. The galls of the Smicronyx weevils formed on Cuscuta campestris are particularly rich in chlorophylls compared to the stem of the parasitic plant. In the present study, we aimed to characterize the photosynthetic activity in the inner and outer gall cortices in comparison to the non-photosynthetic stems and a reference plant (Arabidopsis thaliana). The recorded prompt chlorophyll fluorescence transients were analyzed using JIP test. Detailed analysis of the chlorophyll fluorescence confirmed the presence of actively functioning photosynthetic machinery, especially in the inner cortex of the galls. This photosynthesis, induced by the insect larvae, did not reach the levels of the photosynthetic activity in Arabidopsis thaliana plants. Thylakoid protein complexes were identified by separation with two-dimensional Blue Native/SDS PAGE. It appeared that some of the complexes presented in A. thaliana are missing in C. campestris. We hypothesize that the insect-triggered transition from non-photosynthetic to photosynthetic tissue in the gall is driven by the increased requirements for nutrients related to the larval nutrition. View Full-Text
Keywords: chlorophyll fluorescence; insect galls; JIP-test; parasitic plants chlorophyll fluorescence; insect galls; JIP-test; parasitic plants
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MDPI and ACS Style

Zagorchev, L.; Atanasova, A.; Albanova, I.; Traianova, A.; Mladenov, P.; Kouzmanova, M.; Goltsev, V.; Kalaji, H.M.; Teofanova, D. Functional Characterization of the Photosynthetic Machinery in Smicronix Galls on the Parasitic Plant Cuscuta campestris by JIP-Test. Cells 2021, 10, 1399. https://doi.org/10.3390/cells10061399

AMA Style

Zagorchev L, Atanasova A, Albanova I, Traianova A, Mladenov P, Kouzmanova M, Goltsev V, Kalaji HM, Teofanova D. Functional Characterization of the Photosynthetic Machinery in Smicronix Galls on the Parasitic Plant Cuscuta campestris by JIP-Test. Cells. 2021; 10(6):1399. https://doi.org/10.3390/cells10061399

Chicago/Turabian Style

Zagorchev, Lyuben, Alexandra Atanasova, Ivanela Albanova, Anelia Traianova, Petko Mladenov, Margarita Kouzmanova, Vasilij Goltsev, Hazem M. Kalaji, and Denitsa Teofanova. 2021. "Functional Characterization of the Photosynthetic Machinery in Smicronix Galls on the Parasitic Plant Cuscuta campestris by JIP-Test" Cells 10, no. 6: 1399. https://doi.org/10.3390/cells10061399

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