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Small-RNA Sequencing Reveals Altered Skeletal Muscle microRNAs and snoRNAs Signatures in Weanling Male Offspring from Mouse Dams Fed a Low Protein Diet during Lactation

1
Department of Musculoskeletal & Ageing Science, Institute of Life Course & Medical Sciences, Faculty of Health & Life Sciences, University of Liverpool, Liverpool L7 8TX, UK
2
Chester Medical School, Faculty of Medicine and Life Sciences, University of Chester, Chester CH2 1BR, UK
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School of Biological Sciences, Faculty of Science, University of East Anglia, Norwich NR4 7TJ, UK
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Metabolic Research Laboratories, Wellcome-MRC Institute of Metabolic Science, University of Cambridge, Cambridge CB2 0QQ, UK
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Department of Physiology, School of Medicine and REMEDI, CMNHS, NUI Galway, Galway H91 TK33, Ireland
*
Author to whom correspondence should be addressed.
These authors joint last authorship.
Academic Editor: Giorgio Malpeli
Cells 2021, 10(5), 1166; https://doi.org/10.3390/cells10051166
Received: 13 April 2021 / Revised: 7 May 2021 / Accepted: 9 May 2021 / Published: 11 May 2021
Maternal diet during gestation and lactation affects the development of skeletal muscles in offspring and determines muscle health in later life. In this paper, we describe the association between maternal low protein diet-induced changes in offspring skeletal muscle and the differential expression (DE) of small non-coding RNAs (sncRNAs). We used a mouse model of maternal protein restriction, where dams were fed either a normal (N, 20%) or a low protein (L, 8%) diet during gestation and newborns were cross-fostered to N or L lactating dams, resulting in the generation of NN, NL and LN offspring groups. Total body and tibialis anterior (TA) weights were decreased in weanling NL male offspring but were not different in the LN group, as compared to NN. However, histological evaluation of TA muscle revealed reduced muscle fibre size in both groups at weaning. Small RNA-sequencing demonstrated DE of multiple miRs, snoRNAs and snRNAs. Bioinformatic analyses of miRs-15a, -34a, -122 and -199a, in combination with known myomiRs, confirmed their implication in key muscle-specific biological processes. This is the first comprehensive report for the DE of sncRNAs in nutrition-associated programming of skeletal muscle development, highlighting the need for further research to unravel the detailed molecular mechanisms. View Full-Text
Keywords: maternal protein restriction; muscle development; offspring; microRNAs; sncRNAs; snoRNAs maternal protein restriction; muscle development; offspring; microRNAs; sncRNAs; snoRNAs
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MDPI and ACS Style

Kanakis, I.; Alameddine, M.; Folkes, L.; Moxon, S.; Myrtziou, I.; Ozanne, S.E.; Peffers, M.J.; Goljanek-Whysall, K.; Vasilaki, A. Small-RNA Sequencing Reveals Altered Skeletal Muscle microRNAs and snoRNAs Signatures in Weanling Male Offspring from Mouse Dams Fed a Low Protein Diet during Lactation. Cells 2021, 10, 1166. https://doi.org/10.3390/cells10051166

AMA Style

Kanakis I, Alameddine M, Folkes L, Moxon S, Myrtziou I, Ozanne SE, Peffers MJ, Goljanek-Whysall K, Vasilaki A. Small-RNA Sequencing Reveals Altered Skeletal Muscle microRNAs and snoRNAs Signatures in Weanling Male Offspring from Mouse Dams Fed a Low Protein Diet during Lactation. Cells. 2021; 10(5):1166. https://doi.org/10.3390/cells10051166

Chicago/Turabian Style

Kanakis, Ioannis, Moussira Alameddine, Leighton Folkes, Simon Moxon, Ioanna Myrtziou, Susan E. Ozanne, Mandy J. Peffers, Katarzyna Goljanek-Whysall, and Aphrodite Vasilaki. 2021. "Small-RNA Sequencing Reveals Altered Skeletal Muscle microRNAs and snoRNAs Signatures in Weanling Male Offspring from Mouse Dams Fed a Low Protein Diet during Lactation" Cells 10, no. 5: 1166. https://doi.org/10.3390/cells10051166

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