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Article

Effect of Long-Term 3D Spheroid Culture on WJ-MSC

1
Mossakowski Medical Research Centre, Translational Platform for Regenerative Medicine, Polish Academy of Science, 02-106 Warsaw, Poland
2
Mossakowski Medical Research Centre, Department of Stem Cell Bioengineering, Polish Academy of Sciences, 02-106 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Joni H. Ylostalo
Cells 2021, 10(4), 719; https://doi.org/10.3390/cells10040719
Received: 2 March 2021 / Revised: 19 March 2021 / Accepted: 22 March 2021 / Published: 24 March 2021
(This article belongs to the Special Issue Enhancing Mesenchymal Stem Cells (MSCs) for Therapeutic Purposes)
The aim of our work was to develop a protocol enabling a derivation of mesenchymal stem/stromal cell (MSC) subpopulation with increased expression of pluripotent and neural genes. For this purpose we used a 3D spheroid culture system optimal for neural stem cells propagation. Although 2D culture conditions are typical and characteristic for MSC, under special treatment these cells can be cultured for a short time in 3D conditions. We examined the effects of prolonged 3D spheroid culture on MSC in hope to select cells with primitive features. Wharton Jelly derived MSC (WJ-MSC) were cultured in 3D neurosphere induction medium for about 20 days in vitro. Then, cells were transported to 2D conditions and confront to the initial population and population constantly cultured in 2D. 3D spheroids culture of WJ-MSC resulted in increased senescence, decreased stemness and proliferation. However long-termed 3D spheroid culture allowed for selection of cells exhibiting increased expression of early neural and SSEA4 markers what might indicate the survival of cell subpopulation with unique features. View Full-Text
Keywords: mesenchymal stem cells; mesenchymal stromal cells; 3D culture; neurospheres; spheroids; pluripotency; neural; quiescence mesenchymal stem cells; mesenchymal stromal cells; 3D culture; neurospheres; spheroids; pluripotency; neural; quiescence
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MDPI and ACS Style

Kaminska, A.; Wedzinska, A.; Kot, M.; Sarnowska, A. Effect of Long-Term 3D Spheroid Culture on WJ-MSC. Cells 2021, 10, 719. https://doi.org/10.3390/cells10040719

AMA Style

Kaminska A, Wedzinska A, Kot M, Sarnowska A. Effect of Long-Term 3D Spheroid Culture on WJ-MSC. Cells. 2021; 10(4):719. https://doi.org/10.3390/cells10040719

Chicago/Turabian Style

Kaminska, Agnieszka, Aleksandra Wedzinska, Marta Kot, and Anna Sarnowska. 2021. "Effect of Long-Term 3D Spheroid Culture on WJ-MSC" Cells 10, no. 4: 719. https://doi.org/10.3390/cells10040719

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