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Role of Clathrin Light Chains in Regulating Invadopodia Formation

Department of Infectious Diseases, Virology, University Hospital Heidelberg, Im Neuenheimer Feld 344, 69120 Heidelberg, Germany
Max Planck Institute for Medical Research, 69120 Heidelberg, Germany
German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany
Author to whom correspondence should be addressed.
Cells 2021, 10(2), 451;
Received: 20 December 2020 / Revised: 2 February 2021 / Accepted: 17 February 2021 / Published: 20 February 2021
(This article belongs to the Section Organelle Function)
One of the most fundamental processes of the cell is the uptake of molecules from the surrounding environment. Clathrin-mediated endocytosis (CME) is the best-described uptake pathway and regulates nutrient uptake, protein and lipid turnover at the plasma membrane (PM), cell signaling, cell motility and cell polarity. The main protein in CME is clathrin, which assembles as a triskelion-looking building block made of three clathrin heavy chains and three clathrin light chains. Compared to clathrin heavy chains (CHCs), the role of the two isoforms of clathrin light chains (CLCA and CLCB) is poorly understood. Here, we confirm that the simultaneous deletion of both CLCA/B causes abnormal actin structures at the ventral PM and we describe them, for the first time, as functional invadopodia rather than disorganized actin-cytoskeleton assembly sites. Their identification is based on the occurrence of common invadopodia markers as well as functional invadopodia activity characterized by an increased local proteolytic activity of the extracellular matrix proteins. We demonstrate that CLCA/B deletion impacts the intracellular trafficking and recovery of the matrix metalloproteinase 14 (MMP14) leading to its accumulation at the plasma membrane and induction of invadopodia formation. Importantly, we show that invadopodia formation can be prevented by depletion of MMP14. As such, we propose that CLCA/B regulate invadopodia formation by regulating MMP14 delivery to the plasma membrane. View Full-Text
Keywords: clathrin light chain; invadopodia; MMP14: actin; membrane trafficking clathrin light chain; invadopodia; MMP14: actin; membrane trafficking
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MDPI and ACS Style

Mukenhirn, M.; Muraca, F.; Bucher, D.; Asberger, E.; Cappio Barazzone, E.; Cavalcanti-Adam, E.A.; Boulant, S. Role of Clathrin Light Chains in Regulating Invadopodia Formation. Cells 2021, 10, 451.

AMA Style

Mukenhirn M, Muraca F, Bucher D, Asberger E, Cappio Barazzone E, Cavalcanti-Adam EA, Boulant S. Role of Clathrin Light Chains in Regulating Invadopodia Formation. Cells. 2021; 10(2):451.

Chicago/Turabian Style

Mukenhirn, Markus, Francesco Muraca, Delia Bucher, Edgar Asberger, Elisa Cappio Barazzone, Elisabetta Ada Cavalcanti-Adam, and Steeve Boulant. 2021. "Role of Clathrin Light Chains in Regulating Invadopodia Formation" Cells 10, no. 2: 451.

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