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Article

Rapid Multi-Hybridisation FISH Screening for Balanced Porcine Reciprocal Translocations Suggests a Much Higher Abnormality Rate Than Previously Appreciated

School of Biosciences, University of Kent, Canterbury Kent CT2 7NJ, UK
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Author to whom correspondence should be addressed.
Academic Editor: Francesca Degrassi
Cells 2021, 10(2), 250; https://doi.org/10.3390/cells10020250
Received: 18 December 2020 / Revised: 19 January 2021 / Accepted: 22 January 2021 / Published: 28 January 2021
(This article belongs to the Collection Non-human Chromosome Analysis)
With demand rising, pigs are the world’s leading source of meat protein; however significant economic loss and environmental damage can be incurred if boars used for artificial insemination (AI) are hypoprolific (sub-fertile). Growing evidence suggests that semen analysis is an unreliable tool for diagnosing hypoprolificacy, with litter size and farrowing rate being more applicable. Once such data are available, however, any affected boar will have been in service for some time, with significant financial and environmental losses incurred. Reciprocal translocations (RTs) are the leading cause of porcine hypoprolificacy, reportedly present in 0.47% of AI boars. Traditional standard karyotyping, however, relies on animal specific expertise and does not detect more subtle (cryptic) translocations. Previously, we reported development of a multiple hybridisation fluorescence in situ hybridisation (FISH) strategy; here, we report on its use in 1641 AI boars. A total of 15 different RTs were identified in 69 boars, with four further animals XX/XY chimeric. Therefore, 4.5% had a chromosome abnormality (4.2% with an RT), a 0.88% incidence. Revisiting cases with both karyotype and FISH information, we reanalysed captured images, asking whether the translocation was detectable by karyotyping alone. The results suggest that chromosome translocations in boars may be significantly under-reported, thereby highlighting the need for pre-emptive screening by this method before a boar enters a breeding programme. View Full-Text
Keywords: artificial insemination; fertility; FISH; pig; hypoprolificacy; reciprocal translocation artificial insemination; fertility; FISH; pig; hypoprolificacy; reciprocal translocation
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MDPI and ACS Style

O’Connor, R.E.; Kiazim, L.G.; Rathje, C.C.; Jennings, R.L.; Griffin, D.K. Rapid Multi-Hybridisation FISH Screening for Balanced Porcine Reciprocal Translocations Suggests a Much Higher Abnormality Rate Than Previously Appreciated. Cells 2021, 10, 250. https://doi.org/10.3390/cells10020250

AMA Style

O’Connor RE, Kiazim LG, Rathje CC, Jennings RL, Griffin DK. Rapid Multi-Hybridisation FISH Screening for Balanced Porcine Reciprocal Translocations Suggests a Much Higher Abnormality Rate Than Previously Appreciated. Cells. 2021; 10(2):250. https://doi.org/10.3390/cells10020250

Chicago/Turabian Style

O’Connor, Rebecca E., Lucas G. Kiazim, Claudia C. Rathje, Rebecca L. Jennings, and Darren K. Griffin 2021. "Rapid Multi-Hybridisation FISH Screening for Balanced Porcine Reciprocal Translocations Suggests a Much Higher Abnormality Rate Than Previously Appreciated" Cells 10, no. 2: 250. https://doi.org/10.3390/cells10020250

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