hiPSC-Derived Schwann Cells Influence Myogenic Differentiation in Neuromuscular Cocultures
Abstract
1. Introduction
2. Materials and Methods
2.1. hiPSC Culture
2.2. hiPSC Motoneuron (MN) Differentiation
2.3. hiPSC Schwann Cell (SC) Differentiation
2.4. C2C12 Culture
2.5. Tricultures
2.6. Immunofluorescence Staining and Microscopy
2.7. Image Segmentation and Quantitative Analysis
2.8. Statistical Analysis
3. Results
3.1. Differentiation of Motoneurons (MN) and Schwann Cells (SC) from hiPSC
3.2. Tuning of BMP Activation Improves Robustness of Schwann Cell Differentiation
3.3. Differentiation of hiPSC-Derived Schwann Cells Is Promoted in Triculture Medium
3.4. Tricultures Including hiPSC-Derived SC Can Be Prepared from Frozen Cells in Nine Days
3.5. Cocultures Promote Formation of Myotubes with Increased Cytoplasmic Area
3.6. hiPSC-Derived MN and SC Influence Clustering of AChR on Myotubes In Vitro
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hörner, S.J.; Couturier, N.; Bruch, R.; Koch, P.; Hafner, M.; Rudolf, R. hiPSC-Derived Schwann Cells Influence Myogenic Differentiation in Neuromuscular Cocultures. Cells 2021, 10, 3292. https://doi.org/10.3390/cells10123292
Hörner SJ, Couturier N, Bruch R, Koch P, Hafner M, Rudolf R. hiPSC-Derived Schwann Cells Influence Myogenic Differentiation in Neuromuscular Cocultures. Cells. 2021; 10(12):3292. https://doi.org/10.3390/cells10123292
Chicago/Turabian StyleHörner, Sarah Janice, Nathalie Couturier, Roman Bruch, Philipp Koch, Mathias Hafner, and Rüdiger Rudolf. 2021. "hiPSC-Derived Schwann Cells Influence Myogenic Differentiation in Neuromuscular Cocultures" Cells 10, no. 12: 3292. https://doi.org/10.3390/cells10123292
APA StyleHörner, S. J., Couturier, N., Bruch, R., Koch, P., Hafner, M., & Rudolf, R. (2021). hiPSC-Derived Schwann Cells Influence Myogenic Differentiation in Neuromuscular Cocultures. Cells, 10(12), 3292. https://doi.org/10.3390/cells10123292