Atg5 Regulates Selective Autophagy of the Parental Macronucleus during Tetrahymena Sexual Reproduction
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and Conjugation
2.2. Bioinformatic Analysis
2.3. Construction of HA-ATG5 and HA-ATG8.2 Strains
2.4. ATG5 Knockdown by RNA Interference
2.5. Labeling of Autophagosomal Structures and Lysosomes
2.6. Indirect Immunofluorescence Analysis
2.7. Western Blot Analysis
2.8. Statistical Analysis
3. Results
3.1. Characterization of ATG5 in Tetrahymena
3.2. ATG5 Knockdown Inhibites the PND of paMAC
3.3. Atg5 Mediated the PND of paMAC by Regulating the Function of Atg8.2
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Bo, T.; Kang, Y.; Liu, Y.; Xu, J.; Wang, W. Atg5 Regulates Selective Autophagy of the Parental Macronucleus during Tetrahymena Sexual Reproduction. Cells 2021, 10, 3071. https://doi.org/10.3390/cells10113071
Bo T, Kang Y, Liu Y, Xu J, Wang W. Atg5 Regulates Selective Autophagy of the Parental Macronucleus during Tetrahymena Sexual Reproduction. Cells. 2021; 10(11):3071. https://doi.org/10.3390/cells10113071
Chicago/Turabian StyleBo, Tao, Yu Kang, Ya Liu, Jing Xu, and Wei Wang. 2021. "Atg5 Regulates Selective Autophagy of the Parental Macronucleus during Tetrahymena Sexual Reproduction" Cells 10, no. 11: 3071. https://doi.org/10.3390/cells10113071