Autophagy Is Polarized toward Cell Front during Migration and Spatially Perturbed by Oncogenic Ras
Abstract
:1. Introduction
2. Materials and Methods
2.1. List of Plasmids
2.2. List of Reagents
2.3. Cell Culture and Transfections
2.4. Surface Patterning
2.5. Sample Preparation
2.6. Image Acquisition
2.7. Image Processing with TopoAutophagy Macro
2.8. Tumor-on-Chip 3D Motility Assay
2.9. Statistical Analysis
3. Results and Discussion
3.1. Ras-Transformation Promotes an Active Random Motility in 3D Collagen Gel
3.2. Development of a Micro-Pattern Strategy to Study Spatial-Temporal Localization of Autophagy Organelles during Directional Migration
3.3. LC3 Compartments Are Polarized at the Front toward the Direction of Migration and Spatially Perturbed by Oncogenic Ras
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Singh, M.K.; Zago, G.; Veith, I.; Camonis, J.; Coppey, M.; Parrini, M.C. Autophagy Is Polarized toward Cell Front during Migration and Spatially Perturbed by Oncogenic Ras. Cells 2021, 10, 2637. https://doi.org/10.3390/cells10102637
Singh MK, Zago G, Veith I, Camonis J, Coppey M, Parrini MC. Autophagy Is Polarized toward Cell Front during Migration and Spatially Perturbed by Oncogenic Ras. Cells. 2021; 10(10):2637. https://doi.org/10.3390/cells10102637
Chicago/Turabian StyleSingh, Manish Kumar, Giulia Zago, Irina Veith, Jacques Camonis, Mathieu Coppey, and Maria Carla Parrini. 2021. "Autophagy Is Polarized toward Cell Front during Migration and Spatially Perturbed by Oncogenic Ras" Cells 10, no. 10: 2637. https://doi.org/10.3390/cells10102637