PI3Kγ Mediates Microglial Proliferation and Cell Viability via ROS
Abstract
:1. Introduction
2. Materials and Methods
2.1. Antibodies
2.2. Inhibitors
2.3. Other Reagents
2.4. Animals
2.5. Primary Microglia Cell Isolation and Cultivation
2.6. SDS-PAGE and Immunoblotting
2.7. In Vitro Cell Proliferation and Cell Viability Assessment
2.8. Senescence Assay
2.9. Measurement of ROS
2.10. In Vivo Microglial Proliferation Assay
2.11. Data Analysis and Statistical Procedures
3. Results
3.1. Dose-Dependent Induction of Microglial Proliferation
3.2. Microglial Proliferation Is Mediated by PI3Kγ
3.3. PI3Kγ Improved Viability in LPS-Activated Microglia
3.4. PI3Kγ Mediates Microglial Proliferation via ROS Production
3.5. PI3Kγ Controls Microglial Proliferation via ROS
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Schmidt, C.; Schneble-Löhnert, N.; Lajqi, T.; Wetzker, R.; Müller, J.P.; Bauer, R. PI3Kγ Mediates Microglial Proliferation and Cell Viability via ROS. Cells 2021, 10, 2534. https://doi.org/10.3390/cells10102534
Schmidt C, Schneble-Löhnert N, Lajqi T, Wetzker R, Müller JP, Bauer R. PI3Kγ Mediates Microglial Proliferation and Cell Viability via ROS. Cells. 2021; 10(10):2534. https://doi.org/10.3390/cells10102534
Chicago/Turabian StyleSchmidt, Caroline, Nadine Schneble-Löhnert, Trim Lajqi, Reinhard Wetzker, Jörg P. Müller, and Reinhard Bauer. 2021. "PI3Kγ Mediates Microglial Proliferation and Cell Viability via ROS" Cells 10, no. 10: 2534. https://doi.org/10.3390/cells10102534