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Agronomy 2018, 8(11), 265; https://doi.org/10.3390/agronomy8110265

A Cucumber AGAMOUS-LIKE 15 (AGL15) MADS-Box Gene Mediates Abnormal Leaf Morphology in Arabidopsis

1,2,†
,
3,†
,
2,3
,
3
and
1,*
1
College of Science, Jiangxi Agricultural University, Nanchang 330045, China
2
Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China
3
College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 19 October 2018 / Revised: 9 November 2018 / Accepted: 13 November 2018 / Published: 17 November 2018
(This article belongs to the Section Crop Breeding and Genetics)
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Abstract

The AGL15 subfamily MADS-box proteins play vital roles in various developmental processes, such as floral transition, somatic embryogenesis, and leaf and fruit development. In this work, an AtAGL15 ortholog, CsMADS26, was cloned from cucumber (Cucumis sativus L.). The open reading frame (ORF) of CsMADS26 is 669 bp in length, encoding a predicted protein of 222 amino acids. The CsMADS26 protein contains a highly conserved MADS-box domain and a variable C domain, as well as less conserved I and K domains. Phylogenetic relationship analysis revealed that CsMADS26 was clustered into the AGL15 clade of AGL15 subfamily. Expression analysis based on qRT-PCR showed that CsMADS26 is mainly expressed in reproductive organs including flowers and fruits. Transgenic Arabidopsis plants with ectopic expression of CsMADS26 exhibited curled rosette and cauline leaves, and the leaf size was much smaller than that of wild-type (WT) plants. These results provide clues for the functional characterization of CsMADS26 in the future. View Full-Text
Keywords: cucumber; MADS-box; AGAMOUS-LIKE 15 (AGL15); transgenic Arabidopsis; curled leaf; leaf size cucumber; MADS-box; AGAMOUS-LIKE 15 (AGL15); transgenic Arabidopsis; curled leaf; leaf size
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Zhou, Y.; Ge, L.; Hu, L.; Yang, Y.; Liu, S. A Cucumber AGAMOUS-LIKE 15 (AGL15) MADS-Box Gene Mediates Abnormal Leaf Morphology in Arabidopsis. Agronomy 2018, 8, 265.

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