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Agronomy 2016, 6(1), 20;

NRT2.4 and NRT2.5 Are Two Half-Size Transporters from the Chlamydomonas NRT2 Family

Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Córdoba, Campus de Rabanales, Campus de excelencia internacional (CeiA3), Edif. Severo Ochoa, Córdoba E-14071, Spain
Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, Américo Vespucio 49, Sevilla E-41092, Spain
Departamento de Bioquímica y Biología Molecular, Grupo del Metabolismo del Nitrógeno, Universidad de La Laguna, La Laguna E-38206, Tenerife, Spain
These authors contributed equally to this work
Author to whom correspondence should be addressed.
Academic Editors: Anne Krapp and Bertrand Hirel
Received: 31 October 2015 / Revised: 23 February 2016 / Accepted: 14 March 2016 / Published: 19 March 2016
(This article belongs to the Special Issue Nitrogen Transport and Assimilation in Plants)
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The NRT2 transporters mediate High Affinity Nitrate/NitriteTransport (HAN/NiT), which are essential for nitrogen acquisition from these inorganic forms. The NRT2 proteins are encoded by a multigene family in plants, and contain 12 transmembrane-spanning domains. Chlamydomonas reinhardtii has six NRT2, two of which (NRT2.5 and NRT2.4) are located in Chromosome III, in tandem head to tail. cDNAs for these genes were isolated and their sequence revealed that they correspond to half-size NRT2 transporters each containing six transmembrane domains. NRT2.5 has long N- and C- termini sequences without known homology. NRT2.4 also contains long termini sequences but smaller than NRT2.5. Expression of both studied genes occurred at a very low level, slightly in darkness, and was not modified by the N or C source. Silencing of NRT2.4 by specific artificial miRNA resulted in the inhibition of nitrite transport in the absence of other HANNiT (NRT2.1/NAR2) in the cell genetic background. Nitrite transport activity in the Hansenula polymorpha Δynt::URA3 Leu2 mutant was restored by expressing CrNRT2.4. These results indicate that half-size NRT2 transporters are present in photosynthetic organisms and that NRT2.4 is a HANiT. View Full-Text
Keywords: half-size transporter; nitrite transport; gene expression; Hansenula complementation half-size transporter; nitrite transport; gene expression; Hansenula complementation

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Higuera, J.J.; Calatrava, V.; González, Z.; Mariscal, V.; Siverio, J.M.; Fernández, E.; Galván, A. NRT2.4 and NRT2.5 Are Two Half-Size Transporters from the Chlamydomonas NRT2 Family. Agronomy 2016, 6, 20.

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