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Agronomy 2016, 6(1), 12;

Impact of the Disruption of ASN3-Encoding Asparagine Synthetase on Arabidopsis Development

Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France
The University of Tokyo, Department of Applied Biological Chemistry, Yayoi l-1-1, Bunkyo-ku, 113-8657 Tokyo, Japan
Osaka University, Institute for Protein Research, Suita, Osaka 565-0871, Japan
INRA, IJPB, UMR1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France
Author to whom correspondence should be addressed.
Academic Editors: Anne Krapp and Bertrand Hirel
Received: 22 October 2015 / Revised: 28 January 2016 / Accepted: 4 February 2016 / Published: 14 February 2016
(This article belongs to the Special Issue Nitrogen Transport and Assimilation in Plants)
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The aim of this study was to investigate the role of ASN3-encoded asparagine synthetase (AS, EC during vegetative growth, seed development and germination of Arabidopsis thaliana. Phenotypic analysis of knockout (asn3-1) and knockdown (asn3-2) T-DNA insertion mutants for the ASN3 gene (At5g10240) demonstrated wild-type contents of asparagine synthetase protein, chlorophyll and ammonium in green leaves at 35 days after sowing. In situ hybridization localized ASN3 mRNA to phloem companion cells of vasculature. Young siliques of the asn3-1 knockout line showed a decrease in asparagine but an increase in glutamate. The seeds of asn3-1 and asn3-2 displayed a wild-type nitrogen status expressed as total nitrogen content, indicating that the repression of ASN3 expression had only a limited effect on mature seeds. An analysis of amino acid labeling of seeds imbibed with (15N) ammonium for 24 h revealed that asn3-1 seeds contained 20% less total asparagine while 15N-labeled asparagine ((2-15N)asparagine, (4-15N)asparagine and (2,4-15N)asparagine) increased by 12% compared to wild-type seeds. The data indicate a fine regulation of asparagine synthesis and hydrolysis in Arabidopsis seeds. View Full-Text
Keywords: amino acids; Arabidopsis thaliana; asparagine synthetase; nitrogen metabolism; seed nitrogen amino acids; Arabidopsis thaliana; asparagine synthetase; nitrogen metabolism; seed nitrogen

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Gaufichon, L.; Marmagne, A.; Yoneyama, T.; Hase, T.; Clément, G.; Trassaert, M.; Xu, X.; Shakibaei, M.; Najihi, A.; Suzuki, A. Impact of the Disruption of ASN3-Encoding Asparagine Synthetase on Arabidopsis Development. Agronomy 2016, 6, 12.

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