Fine Mapping of Phytophthora sojae PNJ1 Resistance Locus Rps15 in Soybean (Glycine max (L.) Merr.)

Chen, Bo; Bai, Si; Yang, Ximeng; Niu, Chanyu; Xia, Qiuju; Cai, Zhandong; Jia, Jia; Ma, Qibin; Lian, Tengxiang; Nian, Hai; Cheng, Yanbo

doi:10.3390/agronomy15122736

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Fine Mapping of Phytophthora sojae PNJ1 Resistance Locus Rps15 in Soybean (Glycine max (L.) Merr.)

by

Bo Chen

^1,2,†

,

Si Bai

^1,2,3,†,

Ximeng Yang

^1,2,†,

Chanyu Niu

^1,2,

Qiuju Xia

⁴,

Zhandong Cai

^1,2,

Jia Jia

^1,2,

Qibin Ma

^1,2,

Tengxiang Lian

^1,2,

Hai Nian

^1,2,* and

Yanbo Cheng

^1,2,*

¹

Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Basic Research Center of Excellence for Precise Breeding of Future Crops, College of Agriculture, South China Agricultural University, Guangzhou 510642, China

²

The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, China

³

Qiyang Forestry Bureau, Yongzhou 426100, China

⁴

Rice Molecular Breeding Institute, Granlux Associated Grains, Shenzhen 518023, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Agronomy 2025, 15(12), 2736; https://doi.org/10.3390/agronomy15122736

Submission received: 27 October 2025 / Revised: 18 November 2025 / Accepted: 25 November 2025 / Published: 27 November 2025

(This article belongs to the Special Issue Functional Genomics and Molecular Breeding of Soybeans—2nd Edition)

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Abstract

Phytophthora root rot (PRR), which is caused by the oomycete pathogen Phytophthora sojae (P. sojae), is one of the most devastating diseases affecting global soybean production. The deployment of resistance (Rps) genes through molecular breeding is a sustainable strategy to control this disease. In this study, we finely mapped a novel resistance gene using two recombinant inbred line (RIL) populations: one comprising 248 F_8:11 lines from a cross between the resistant cultivar ‘Guizao 1’ and the susceptible ‘B13’, and another consisting of 196 F_7:8 lines from a cross between ‘Wayao’ (resistant) and ‘Huachun 2’ (susceptible). The gene in ‘Guizao 1’, designated as Rps15, was delimited to a 78 kb genomic interval on chromosome 3 (bin31), spanning the physical positions from 4,292,416 to 4,370,772 bp. This region contains eight predicted genes. Similarly, the resistance locus in ‘Wayao’ was mapped to a broader region on chromosome 3 (approximately 324 kb; 3,968,039–4,292,863 bp), which encompasses 16 genes. Expression analysis via quantitative real-time PCR of the candidate genes suggested that Glyma.03g036000 is likely involved in the resistance response to PRR. The fine mapping of this novel Rps locus provides a foundation for the future cloning of Rps15 and can be expected to accelerate the development of P. sojae-resistant soybean cultivars through marker-assisted selection.

Keywords: soybean; Phytophthora root rot (PRR); fine mapping; recombinant inbred lines (RILs); resistance gene; Rps15

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MDPI and ACS Style

Chen, B.; Bai, S.; Yang, X.; Niu, C.; Xia, Q.; Cai, Z.; Jia, J.; Ma, Q.; Lian, T.; Nian, H.; et al. Fine Mapping of Phytophthora sojae PNJ1 Resistance Locus Rps15 in Soybean (Glycine max (L.) Merr.). Agronomy 2025, 15, 2736. https://doi.org/10.3390/agronomy15122736

AMA Style

Chen B, Bai S, Yang X, Niu C, Xia Q, Cai Z, Jia J, Ma Q, Lian T, Nian H, et al. Fine Mapping of Phytophthora sojae PNJ1 Resistance Locus Rps15 in Soybean (Glycine max (L.) Merr.). Agronomy. 2025; 15(12):2736. https://doi.org/10.3390/agronomy15122736

Chicago/Turabian Style

Chen, Bo, Si Bai, Ximeng Yang, Chanyu Niu, Qiuju Xia, Zhandong Cai, Jia Jia, Qibin Ma, Tengxiang Lian, Hai Nian, and et al. 2025. "Fine Mapping of Phytophthora sojae PNJ1 Resistance Locus Rps15 in Soybean (Glycine max (L.) Merr.)" Agronomy 15, no. 12: 2736. https://doi.org/10.3390/agronomy15122736

APA Style

Chen, B., Bai, S., Yang, X., Niu, C., Xia, Q., Cai, Z., Jia, J., Ma, Q., Lian, T., Nian, H., & Cheng, Y. (2025). Fine Mapping of Phytophthora sojae PNJ1 Resistance Locus Rps15 in Soybean (Glycine max (L.) Merr.). Agronomy, 15(12), 2736. https://doi.org/10.3390/agronomy15122736

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Fine Mapping of Phytophthora sojae PNJ1 Resistance Locus Rps15 in Soybean (Glycine max (L.) Merr.)

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