Next Article in Journal
Accurate Characterization of Soil Moisture in Wheat Fields with an Improved Drought Index from Unmanned Aerial Vehicle Observations
Next Article in Special Issue
The Synergistic Optimization of Rice Yield, Quality, and Profit by the Combined Application of Organic and Inorganic Nitrogen Fertilizers
Previous Article in Journal
Effects of Scallop Shells and Starfish (Asterias amurensis) on Stabilization of Metalloid (As) and Heavy Metal (Pb and Zn)-Contaminated Soil
Previous Article in Special Issue
Connecting the Dots between GmPERK-1 and Enhanced Grain Weight in Glycine max
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Agronomy
Volume 14
Issue 8
10.3390/agronomy14081782
agronomy-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

The Impact of the Growth Regulators and Cultivation Conditions of Temporary Immersion Systems (TISs) on the Morphological Characteristics of Potato Explants and Microtubers

by
Dias Daurov
1,2,*,
Ainash Daurova
1,
Zagipa Sapakhova
1,2,
Rakhim Kanat
1,2,
Dana Akhmetzhanova
1,
Zhanar Abilda
1,
Maxat Toishimanov
1,
Nurgul Raissova
1,
Murat Otynshiyev
1,
Kabyl Zhambakin
1 and
Malika Shamekova
1,2,*
1
Laboratory of Breeding and Biotechnology, Institute of Plant Biology and Biotechnology, Almaty 050040, Kazakhstan
2
Tanir Research Laboratory, 75B Al-Farabi Avenue, Almaty 050060, Kazakhstan
*
Authors to whom correspondence should be addressed.
Agronomy 2024, 14(8), 1782; https://doi.org/10.3390/agronomy14081782
Submission received: 10 July 2024 / Revised: 30 July 2024 / Accepted: 13 August 2024 / Published: 14 August 2024
(This article belongs to the Special Issue New Inspirations concerning Breeding and Cultivation Practice to Enhance Crop Productivity and Their Essentials)
Browse Figures
Versions Notes

Abstract

Potatoes (Solanum tuberosum L.) constitute one of the most economically important annual crops. In terms of tissue culture, potato microtubers (MTs) have a number of advantages over conventional plants. These advantages include their small size, which greatly facilitates storage, transport, and germplasm exchange compared to in vitro plants. One effective solution for the production and mass propagation of healthy MTs is the use of temporary immersion systems (TISs). In this study, in a SETISTM system containing kinetin/gibberellic acid (GA)/indole-3-butyric acid (IBA) hormones, we investigated the effects of different nutrient media on the morphological characteristics of potato explants and MTs. We determined the optimal cycling duration (3 h) with an immersion frequency of 2 min. The results revealed that the optimal nutrient medium for culturing single-node potato explants in a SETISTM bioreactor was the M7 medium containing kinetin (2 mg/L), GA (0.5 mg/L), and IBA (0.5 mg/L). The optimal nutrient medium for obtaining potato MTs was the M1 medium (hormone-free) with a high concentration of sucrose (9%) at 18 °C under dark growing conditions. Thus, a universal nutrient medium, employed in a bioreactor, was selected for the mass propagation of potato MTs for both domestic and foreign potato varieties.
Keywords: potato (Solanum tuberosum L.); microtubers; temporary immersion system; varieties; nutrient medium; immersion frequency potato (Solanum tuberosum L.); microtubers; temporary immersion system; varieties; nutrient medium; immersion frequency

Share and Cite

MDPI and ACS Style

Daurov, D.; Daurova, A.; Sapakhova, Z.; Kanat, R.; Akhmetzhanova, D.; Abilda, Z.; Toishimanov, M.; Raissova, N.; Otynshiyev, M.; Zhambakin, K.; et al. The Impact of the Growth Regulators and Cultivation Conditions of Temporary Immersion Systems (TISs) on the Morphological Characteristics of Potato Explants and Microtubers. Agronomy 2024, 14, 1782. https://doi.org/10.3390/agronomy14081782

AMA Style

Daurov D, Daurova A, Sapakhova Z, Kanat R, Akhmetzhanova D, Abilda Z, Toishimanov M, Raissova N, Otynshiyev M, Zhambakin K, et al. The Impact of the Growth Regulators and Cultivation Conditions of Temporary Immersion Systems (TISs) on the Morphological Characteristics of Potato Explants and Microtubers. Agronomy. 2024; 14(8):1782. https://doi.org/10.3390/agronomy14081782

Chicago/Turabian Style

Daurov, Dias, Ainash Daurova, Zagipa Sapakhova, Rakhim Kanat, Dana Akhmetzhanova, Zhanar Abilda, Maxat Toishimanov, Nurgul Raissova, Murat Otynshiyev, Kabyl Zhambakin, and et al. 2024. "The Impact of the Growth Regulators and Cultivation Conditions of Temporary Immersion Systems (TISs) on the Morphological Characteristics of Potato Explants and Microtubers" Agronomy 14, no. 8: 1782. https://doi.org/10.3390/agronomy14081782

APA Style

Daurov, D., Daurova, A., Sapakhova, Z., Kanat, R., Akhmetzhanova, D., Abilda, Z., Toishimanov, M., Raissova, N., Otynshiyev, M., Zhambakin, K., & Shamekova, M. (2024). The Impact of the Growth Regulators and Cultivation Conditions of Temporary Immersion Systems (TISs) on the Morphological Characteristics of Potato Explants and Microtubers. Agronomy, 14(8), 1782. https://doi.org/10.3390/agronomy14081782

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop