Identification of Olea europaea CBF/DREB1 Family Genes in Abnormal Temperature Stress Response
Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsI congratulate the authors for their work and their manuscript. The manuscript "Identification of Olea europaea CBF/DREB1 Family Genes in Abnormal Temperature Stress Response" by Hai and co-workers is an important study on genes responsible for various essential traits in olive cultivation. This helps to understand the selection of olive cultivars to adapt to climatic changes, especially temperature stress. The manuscript is well written and the data is clearly presented. I have only a few minor suggestions, which are listed below.
Line 52: Please add the percentage of olive cultivation compared to other fruit crops in the areas you mention.
Line 73 (as an example): Insert a space between the words and a reference.
Line 103: Please explain or add a reference as to why you analysed these two varieties?
Line 106: Please explain or amend the meaning of "seedlings"; These plants are seedlings? If the plants are vegetatively propagated, they are not seedlings.
Table S1: Please add references for the primers you used in your study in Table S1.
Line 338: Delete "." before ","
Author Response
Please see attachment
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for AuthorsAdd one space between the text and the references.
L18. …cold tolerance. The analysis of the olive genome identified four nuclear OeCBF…
L19. …frames (ORF) were 552~684 bp in length, encoded for proteins of …
L20. …(pI) between…
L20-22. Delete “Four OeCBFs…other.”
L22. …was significant different in…
L24. …in below ground…
L25. Delete “inducing”
L27. Comparison of …
L29. …Picholine (more tolerant) than that of Arbequina (less tolerant).
L37. Oleaàitalics
L39. Rephrase
L44-45. Rephrase
L48. You can also add the following reference: Simos er al. 2023 (doi.org/10.3390/microorganisms11030770) or Mermigka et al. 2023 (doi.org/10.3390/metabo13111126)
L56-58. Olive breeding for high-yield and resistance have attracted widespread attention [14], which is based …
L61. … TFs, the Apetala2/Ethylene Responsive Factor (AP2/ERF) superfamily…
L64. The C-repeat Binding Factor/Dehydration Responsive Element Binding (CBF/DREB1) primarily…
L71. … [17-19]. Today …
L76. …components: the CBF, the …
L77. ArabidopsisàItalics
L85. This facilitated the identification …
L87. …families, that include 128 genes linked to the…
L88. …of olive cold stress, our study…
L90. …to elucidate their role…
L91. …context of cold stress.
L96. …for analyses.
L97. …expression analyses, …
L98. …olive plants were grown…
L100. …in China. Plant…
L102. Each sample consisted of material from at least…
L102. …response analyses, …
L104. …were transplanted to pots, left to adapt for about…
L106. Then, the …
L107. …were sampled separately …
L108. From each temperature treatment, samples were taken in triplicates. (Is this what you meant?)
L194. …OeCBF is located on…
L196-197. …existence of a duplication pair between the OeCBF4…
L202. …we constructed an unrooted phylogenetic…
L210. …different plant species.
L223. To what is the percentage refering to? Describe.
L225. O. europaea à Full name and in italics.
L231. … OeCBF1 was categorized in a distinct phylogenetic group from the rest of OeCBF genes (Figure 1), its gene structure resembled more to that of OeCFB4.
Though, how did you conclude to that? Because it has one intron similarly to CBF4? I do not think this is enough to conclude that they are similar. Please modify accordingly.
L235-236. O. europaea à Full name and in italics
L239. The four OeCBFs found to possess an AP2/ERF family structure (Figure 3a).
L241-L242. Rephrase, there is no meaning to this.
L242. …including these from olive, contained…
L253. Provide the full names for the species and in italics.
L256. The 2000 bp regions upstream of …
L260. Prevalent and were found…
L264. Provide the full name for the MeJA.
L267. …contained a low-temperature…
L269. …inducible element…
L275. …function of OeCBF…
L275-278. In what conditions?
L279. …significantly across different…
L283. …, with relative expression…
L287. …Arbequina that was…
L296-301. I cannot understand what the b,d are. Give more details.
L331. All the gene expression differences…
L331-335. I cannot understand what the b,d,f,h,j,l are. Give more details.
L338-340. Rephrase.
L344-345. …implying the potential…
L348. …[23]. The prediction of subcellular localization indicated that OeCBF1 is primarily expressed in the…
L358-L364. Delete “We…event.”
L361. …(Figure 2), though we found differences in secondary structure, OeCBF1…
Is that what you wanted to write?
L362. (Table 2)
L365-L371. What is the DSAWR and how did you find that? You did not make any reference to the results section. Rephrase.
L378. Especially OeCBF2
L386. …levels in the resistant cultivar Mironovskay 808’ (M808) than in the less resistant cultivar Chinese Spring (CS) and that is why it was suggested as a pivotal gene for determining wheat resistance [52]. Similarly, in tea…
Comments on the Quality of English Language-
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
