The Effects of Different Organic Materials and Cover Thicknesses on the Early Growth and Antioxidant Response of Direct-Seeded Rice

Abstract

Direct-seeded rice (Oryza sativa) (DSR) is gaining increasing acceptance worldwide because it saves both time and labor. A covering treatment is a simple method to improve early plant growth under direct-seeding conditions. Herein, field experiments were conducted to study the effects of different powdered organic covering materials, i.e., organic fertilizer, seedling substrate, nutrient soil A, and nutrient soil B. In addition, the effects of different covering thicknesses were studied, including 5, 10, 15, and 20 mm, on the germination and early growth of DSR varieties. The two rice varieties Wufengyou 286 and Zhongjiazao 17 were used for this study. The seedling substrate at 15 mm and nutrient soil A at 5 mm covering thickness significantly increased the rate of germination of Wufengyou 286 by 43.93% and 40.26%, respectively, compared to the control treatment. Organic fertilizer, seedling substrate, and nutrient soil A at covering thicknesses of 5 mm significantly increased the germination rate by 42.57%, 38.62%, and 40.10%, respectively, compared to the control. Notably, all the treatments increased the emergence rate, with Wufengyou 286 exhibiting the most pronounced effect. The seedling substrate at 10 mm covering thickness significantly increased the rate of emergence of Wufengyou286 by 15.42% compared to the control. Similarly, the seedling substrate at 10 mm covering thickness gave the best results by increasing the rate of emergence of Zhongjiazao 17 by 10.85% compared to the control. On average, the rate of emergence of Wufengyou 286 and Zhongjiazao 17 under the experimental treatment increased by 11.81% and 6.45%, respectively, compared to the control. The appropriate cover treatment enhanced the early growth of DSR by improving its morphological attributes, such as plant height and dry weight, and regulating the physio-biochemical responses, such as the production of antioxidants and photosynthetic pigments. The seedling growth rate of both varieties decreased as the thickness increased. This study elucidated the effects of different covering materials on the early growth of DSR, which can further guide their actual production.

1. Introduction

Rice (Oryza sativa) is a fundamental dietary component for more than half of the world’s population [1]. Direct-seeded rice (DSR) is gaining acceptance worldwide because it saves time and labor [2]. However, the large-scale implementation of DSR by farmers is hindered by numerous challenges under adverse environments [3]. Studies have hypothesized that the exposure of the seeds on the mud surface after direct-seeding often makes them susceptible to birds and pests. This exposure often hinders seedling growth and results in uneven plant population and development overall [4,5]. Moreover, direct-seeding leads to significant challenges in achieving uniform seedling growth because of the impact of rain erosion and extreme temperatures [6]. Therefore, it is important to address these issues during the process of seed germination and establishment of seedlings.

Various agronomic strategies to alleviate the challenges posed by the growth and yield of direct-seeding rice have been implemented in previous studies. These strategies include using growth regulatory substances, such as macro- and micro-elements, plant growth regulators, and chemical agents, which can all be applied as seed coatings [7,8,9,10,11]. The use of growth regulatory substances aids in protecting the seeds and enhancing their resistance to environmental stress, thereby improving the germination rate and growth rate of the seedlings [12]. In addition, seed priming in DSR has led to an acceleration in the germination and emergence process (within 1–3 days), which culminates in more consistent and robust seedling growth [13].

Covering the seeds is a simple method to improve early plant growth under direct-seeding conditions [14]. In particular, covering the seeds with soil enhances the plant’s resistance to lodging and bolsters the tolerance of rice to drought because the roots can absorb water from the deeper layers of the soil [15]. Although appropriate high sowing depths or cover thicknesses are favorable for improving germination and the establishment of rice seedlings, deeper sowing depths may also reduce germination and the establishment of seedlings [16]. The widespread use of direct-seeding technology is significantly hindered by poor emergence and weak seedling establishment that results from the deep soil covering conditions [17]. Thus, the covering treatment is a good way to improve the germination of seeds and the early plant growth of the seedlings under direct-seeding conditions.

Therefore, in this study, we conducted a field experiment to study the effects of different organic materials and the covering thicknesses of the organic materials on the germination and early growth of DSR. This study hypothesized that the different organic materials and their covering thicknesses impact the germination and early growth of seedlings. Thus, we complemented our analyses of the best management model for the covering thicknesses of the organic materials. The objective of this study was to determine the optimal organic material and covering thickness for DSR, which provides guidelines for the production of DSR.

2. Materials and Methods

2.1. Experimental Materials

The experimental materials for the mechanical DSR included organic fertilizers (O), seedling substrate (S), nutrient soil A (NA), and nutrient soil B (NB—a 2:1 mixture of nutrient soil A and organic fertilizer). The experimental materials were those usually used in the rice planting process in Jiangxi, China. The rice varieties used were the conventional rice variety Zhongjiazao 17 and the hybrid rice variety Wufengyou 286. Four different thicknesses of ditch openers with heights of 5 mm, 10 mm, 15 mm, and 20 mm were designed and processed using three-dimensional (3D) printing technology to produce different cover thicknesses of the organic materials. Frames of 40 mm × 500 mm were subsequently processed using acrylic boards of different thicknesses to ensure that the organic materials were uniformly covered.

2.2. Experimental Details

The field experiment was conducted in the Jiangxi Agricultural University experimental farm (Nanchang, China) in March and April 2023. The experimental field block was prepared following the requirements of a mechanical direct-seeded field block. Four different depths of seed furrows were opened on the mud surface before sowing using a ditch opener. The furrow length was 500 mm, in which 100 germinated rice seeds of the test variety were placed evenly in each of the four furrows. Acrylic frames of different thicknesses were placed in the seed furrows of their corresponding depth after sowing, followed by even covering using the four organic materials (organic fertilizer, seedling substrate, nutrient soil A, and nutrient soil B). Excess material on the surface was scraped off using a scraper. This study was conducted with two varieties (Zhongjiazao 17 and Wufengyou 286) and 17 treatments, which included the CK: no organic materials were applied; organic fertilizer with 5, 10, 15, and 20 covered depth denoted as O5, O10, O15, and O20; seedling substrate with 5, 10, 15, and 20 covered depth denoted as S5, S10, S15, and S20; nutrient soil A with 5, 10, 15, and 20 covered depth denoted as NA5, NA10, NA15, and NA20; and nutrient soil B with 5, 10, 15, and 20 covered depth denoted as NB5, NB10, NB15, and NB20. The plot area was 1 m² per treatment. Mud surface sowing was used as the control, i.e., the plots were not covered (Figure 1).

3. Sampling and Measurements

The seed germination and dynamics of emergence were checked daily after the seeds had been sown for 14 days. The seedling quality of different treatments was measured at the three-leaf-one-heart stage. The quality measurements included the dry weight of the roots, stems, and leaves, plant height, leaf age, and their physiological and biochemical indicators.

3.1. Determination of the Germination Rate and Morphological Traits

The germination traits were measured as previously described [7]. The germination rate was recorded daily after sowing until there was no significant change in the number. The germination rate of the control treatment was recorded when the roots and shoots of the seedlings reached 3 mm in length. When the treatments that had been covered with organic material cover sprouted, this was recorded when the seedlings emerged. Ten seedlings were randomly selected from each treatment, and there were six replicates during sampling. The morphological traits that were determined included the plant height, leaf age, dry weight of the seedlings, and dry weight per unit seedling height.

3.2. Determination of the Antioxidant Response Parameters

Fresh seedling samples were gathered and preserved at −80 °C for future use to measure the parameters of the antioxidant response. The samples were then pulverized in liquid nitrogen, and the plant extracts were isolated using phosphate buffer (pH 7.8). The plant extracts were kept below 4 °C, and the antioxidant enzyme activities and the contents of malondialdehyde (MDA) and soluble proteins were quantified as previously described [18]. In addition, the contents of hydrogen peroxide (H₂O₂) and ascorbic acid were quantified as previously described [19]. The activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were expressed as U min⁻¹ g⁻¹ FW. The contents of MDA, soluble protein, and ascorbic acid were expressed as µmol g⁻¹ FW, mg g⁻¹ FW, and µg g⁻¹ FW, respectively.

3.3. Determination of the Contents of Chlorophyll and Carotenoids

The contents of chlorophyll and carotenoids were quantified as previously described [20]. Fresh seedling samples were immersed in 6 mL of 95% ethanol and incubated in the dark at 4 °C for 24 h. The samples were then centrifuged at 5000 rpm for 10 min at 4 °C. The absorbance of the supernatant was then measured at wavelengths of 665, 649, and 470 nm, respectively. The contents of chlorophyll and carotenoids were expressed as mg g⁻¹ FW.

3.4. Statistical Analyses

All the data were documented in Microsoft Excel 2019 (Redmond, WA, USA) and subsequently subjected to a one-way analysis of variance (ANOVA) and multiple comparisons using Statistix 8.0 (Tallahassee, FL, USA). The average values of each treatment were compared using the Tukey HSD test at a 5% probability level. A correlation analysis was conducted using SPSS 21.0 (IBM, Inc., Armonk, NY, USA), and the figures were generated using Origin 2021 (OriginLab, Northampton, MA, USA).

4. Results

4.1. Germination Attributes

The variety (V), material (M), treatment (T), M × T, and V × M × T significantly affected the germination rate (

Table 1. Analysis of variance (ANOVA) of the parameters studied.

Parameter	V	M	V × M	T	V × T	M × T	V × M × T
Plant height	**	**	ns	**	**	**	ns
Dry weight of the seedlings	ns	**	**	**	**	**	**
Dry weight per unit seedling height	ns	**	**	**	*	ns	*
Germination rate	*	**	ns	**	ns	**	ns
Emergence rate	ns	*	ns	**	ns	ns	ns
CAT activity	**	**	**	**	**	**	**
SOD activity	**	**	**	**	**	**	**
MDA contents	*	**	**	**	**	**	**
POD activity	**	**	**	**	**	**	**
H2O2 content	ns	**	**	**	**	**	**
Soluble protein content	**	**	**	**	**	**	**
Ascorbic acid content	*	**	**	**	**	**	**
Chlorophyll a content	*	**	*	**	**	**	**
Chlorophyll b content	*	**	ns	**	**	**	**
Carotenoids content	**	**	**	**	**	**	**
Chlorophyll content	*	**	*	**	**	**	**
Chlorophyll a:b	ns	**	ns	**	**	**	ns

Note: * p < 0.05. ** p < 0.01. ns, non-significant at p > 0.05 (Least Significant Difference [LSD] test). V, variety; M, material; T, treatment; CAT, catalase; SOD, superoxide dismutase; MDA, malondialdehyde; POD, peroxidase; H₂O₂, hydrogen peroxide.

). All the treatments of each material resulted in a significant increase in the germination rate for both varieties. The S5 and NA5 treatments significantly increased the germination rate of Wufengyou 286 by 43.93% and 40.26%, respectively, compared to the CK. The O5, S5, and NA5 treatments significantly increased the germination rate of Zhongjiazao 17 by 42.57%, 38.62%, and 40.10%, respectively, compared to the CK. Notably, the rate of seedling emergence decreased as the thickness of the different cover materials increased in both varieties (

Table 2. The germination and rates of emergence of the direct-seeded rice under different cover materials and different cover thickness treatments.

Variety	Material	Treatment	Germination Rate (%)	Emergence Rate (%)
Wufengyou 286	CK	0	66.94 ± 6.84 de	84.93 ± 3.44 f
	O	5	87.22 ± 6.05 ab	92.50 ± 1.74 bcde
	O	10	33.61 ± 6.13 g	91.10 ± 1.84 de
	O	15	40.56 ± 3.06 fg	95.10 ± 0.93 abcd
	O	20	49.72 ± 15.92 f	93.27 ± 1.82 abcde
	S	5	95.83 ± 0.83 a	96.83 ± 0.23 ab
	S	10	94.72 ± 3.20 a	98.03 ± 1.58 a
	S	15	81.67 ± 0.48 abcd	94.57 ± 1.72 abcd
	S	20	85.00 ± 3.00 abc	95.63 ± 2.03 abcd
	NA	5	93.89 ± 1.69 a	97.97 ± 0.73 a
	NA	10	87.22 ± 2.17 ab	98.07 ± 0.58 a
	NA	15	71.67 ± 1.44 bcd	96.50 ± 0.75 abc
	NA	20	50.28 ± 5.62 f	88.63 ± 1.74 ef
	NB	5	86.11 ± 5.30 abc	97.40 ± 0.80 ab
	NB	10	68.06 ± 4.84 de	95.70 ± 1.46 abcd
	NB	15	70.56 ± 3.09 cde	96.43 ± 1.45 abc
	NB	20	55.00 ± 4.74 ef	91.70 ± 3.49 cde
Zhongjiazao 17	CK	0	56.11 ± 4.72 c	87.93 ± 3.34 d
	O	5	80.00 ± 3.63 a	93.77 ± 0.47 abc
	O	10	26.11 ± 5.70 gh	91.77 ± 2.19 bcd
	O	15	19.17 ± 2.41 h	88.23 ± 3.80 cd
	O	20	32.78 ± 2.78 fg	95.53 ± 1.13 ab
	S	5	77.78 ± 2.17 a	93.20 ± 0.74 abcd
	S	10	76.11 ± 3.09 a	97.47 ± 0.30 a
	S	15	60.00 ± 2.41 bc	94.07 ± 1.59 ab
	S	20	48.06 ± 3.92 cde	92.23 ± 1.91 abcd
	NA	5	78.61 ± 2.27 a	96.43 ± 1.07 ab
	NA	10	68.33 ± 2.50 ab	96.00 ± 0.98 ab
	NA	15	35.00 ± 1.73 fg	93.50 ± 1.91 abcd
	NA	20	39.44 ± 5.53 def	90.90 ± 2.92 bcd
	NB	5	70.28 ± 8.66 ab	95.17 ± 1.22 ab
	NB	10	50.00 ± 5.42 cd	93.67 ± 1.69 abc
	NB	15	50.83 ± 5.46 cd	94.10 ± 2.57 ab
	NB	20	36.94 ± 2.27 efg	91.63 ± 1.10 bcd

Note: Values are presented as the mean ± SD, followed by different letters that denote the level of significance based on the least significant difference (LSD) test (p < 0.05). CK, control; O, organic fertilizer; S, seedling substrate; NA, nutrient soil A; NB, nutrient soil B.

).

The material (M) and treatment (T) significantly affected the rate of emergence (Table 1). All the treatments increased the rate of rice emergence, and the most pronounced rate was observed with Wufengyou 286. The S10 treatment significantly increased the rate of emergence of Wufengyou 286 by 15.42% compared to the CK. Similarly, the S10 treatment increased the rate of emergence of Zhongjiazao 17 by 10.85% compared to the CK (Table 2).

4.2. Morphological Attributes

The variety (V), material (M), treatment (T), V × T, and M × T significantly affected the plant height (Table 1). All the treatments of each material resulted in a significant increase in the height of Wufengyou 286, with the best results observed from the seedling substrate treatment. Notably, the S20 treatment significantly increased the plant height by 27.76% compared to the CK. All the treatments of each material except those with nutrient soil B resulted in a significant increase in the height of Zhongjiazao 17, and the seedling substrate treatment was the most effective. Similarly, the S20 treatment significantly increased the plant height by 17.98% compared to the CK (

Table 3. The plant height and leaf age of direct-seeded rice under different cover materials and different cover thickness treatments.

Variety	Material	Treatment	Plant Height (cm)	Leaf Age
Wufengyou 286	CK	0	13.94 ± 0.30 g	3.23 ± 0.14 d
	O	5	17.19 ± 0.56 ab	3.94 ± 0.10 a
	O	10	13.96 ± 0.46 g	3.19 ± 0.12 d
	O	15	15.07 ± 0.40 def	3.28 ± 0.15 d
	O	20	16.12 ± 0.38 cd	3.31 ± 0.13 d
	S	5	15.99 ± 0.36 cd	3.95 ± 0.05 a
	S	10	16.15 ± 0.31 bc	3.67 ± 0.12 abc
	S	15	17.59 ± 0.26 a	3.40 ± 0.01 cd
	S	20	17.81 ± 0.46 a	3.21 ± 0.08 d
	NA	5	15.36 ± 0.42 cde	3.73 ± 0.11 ab
	NA	10	15.61 ± 0.41 cde	3.43 ± 0.13 bcd
	NA	15	15.78 ± 0.37 cde	3.14 ± 0.08 d
	NA	20	14.12 ± 0.38 fg	3.45 ± 0.07 bcd
	NB	5	15.48 ± 0.23 cde	3.35 ± 0.13 d
	NB	10	15.32 ± 0.40 cde	3.37 ± 0.15 cd
	NB	15	14.81 ± 0.40 efg	3.18 ± 0.11 d
	NB	20	15.69 ± 0.25 cde	3.37 ± 0.07 cd
Zhongjiazao 17	CK	0	13.96 ± 0.50 de	3.21 ± 0.11 defgh
	O	5	15.72 ± 0.22 ab	3.76 ± 0.12 ab
	O	10	13.62 ± 0.47 de	3.09 ± 0.08 gh
	O	15	13.51 ± 0.55 de	3.15 ± 0.13 efgh
	O	20	15.62 ± 0.39 ab	3.57 ± 0.12 bc
	S	5	16.05 ± 0.29 ab	3.95 ± 0.11 a
	S	10	16.06 ± 0.29 ab	3.51 ± 0.13 bcd
	S	15	16.25 ± 0.26 ab	3.62 ± 0.16 bc
	S	20	16.47 ± 0.26 a	3.38 ± 0.07 cdefg
	NA	5	15.31 ± 0.32 bc	3.55 ± 0.16 bc
	NA	10	15.37 ± 0.20 bc	3.56 ± 0.13 bc
	NA	15	13.33 ± 0.44 e	2.95 ± 0.04 h
	NA	20	14.12 ± 0.38 de	3.45 ± 0.07 bcde
	NB	5	14.43 ± 0.37 cd	3.63 ± 0.15 abc
	NB	10	13.64 ± 0.31 de	3.12 ± 0.11 fgh
	NB	15	13.61 ± 0.22 de	3.12 ± 0.07 fgh
	NB	20	13.92 ± 0.24 de	3.43 ± 0.13 cdef

Note: Values are presented as the mean ± SD, followed by different letters that denote the level of significance based on the least significant difference (LSD) test (p < 0.05). CK, control; O, organic fertilizer; S, seedling substrate; NA, nutrient soil A; NB, nutrient soil B.

).

The material (M), treatment (T), and M × T significantly affected the leaf age (Table 1). The O5, S5, S10, and NA5 treatments significantly increased the leaf age of Wufengyou 286 compared to the CK, and the O5 treatment caused the largest increase of 21.98%. In contrast, all the treatments of each material resulted in a significant increase in the leaf age of Zhongjiazao17, and the S5 treatment caused the largest increase of 23.05% (Table 3).

The material (M), variety (V) × M, treatment (T), V × T, M × T, and V × M × T significantly affected the dry weight of the seedlings (Table 1). The treatments covered with seedling substrate positively affected the dry weight of Wufengyou286 seedlings, with S5 increasing the dry weight by 51.85% compared to the CK. The O5 and S5 treatments significantly increased the dry weight of Zhongjiazao 17 seedlings by 40.74% and 18.52%, respectively, compared to the CK (

Table 4. The dry weight of seedlings and dry weight per unit seedling height of direct-seeded rice under different cover materials and different cover thickness treatments.

Variety	Material	Treatment	Seedling Dry Weight	Dry Weight Per Unit Seedling Height
Wufengyou 286	CK	0	0.27 ± 0.01 efgh	0.021 ± 0.001 b
	O	5	0.37 ± 0.01 b	0.021 ± 0.002 bc
	O	10	0.26 ± 0.02 fghi	0.019 ± 0.002 bcd
	O	15	0.24 ± 0.01 ij	0.015 ± 0.000 fg
	O	20	0.25 ± 0.01 ghij	0.015 ± 0.001 g
	S	5	0.41 ± 0.02 a	0.025 ± 0.000 a
	S	10	0.28 ± 0.01 def	0.018 ± 0.001 cdefg
	S	15	0.33 ± 0.01 c	0.019 ± 0.001 bcd
	S	20	0.31 ± 0.01 cd	0.018 ± 0.001 cdefg
	NA	5	0.29 ± 0.01 de	0.019 ± 0.002 bcd
	NA	10	0.25 ± 0.01 hij	0.015 ± 0.001 fg
	NA	15	0.28 ± 0.01 defg	0.018 ± 0.001 bcdef
	NA	20	0.25 ± 0.00 hij	0.017 ± 0.001 defg
	NB	5	0.28 ± 0.00 defg	0.019 ± 0.000 bcde
	NB	10	0.23 ± 0.01 j	0.016 ± 0.001 efg
	NB	15	0.25 ± 0.01 ghij	0.016 ± 0.001 defg
	NB	20	0.27 ± 0.01 efgh	0.017 ± 0.000 cdefg
Zhongjiazao 17	CK	0	0.27 ± 0.01 def	0.020 ± 0.000 bc
	O	5	0.38 ± 0.00 a	0.023 ± 0.000 a
	O	10	0.26 ± 0.02 def	0.018 ± 0.000 ab
	O	15	0.22 ± 0.01 ef	0.016 ± 0.001 c
	O	20	0.30 ± 0.02 def	0.019 ± 0.002 bc
	S	5	0.32 ± 0.01 ab	0.020 ± 0.002 abc
	S	10	0.29 ± 0.01 abcde	0.018 ± 0.001 ab
	S	15	0.30 ± 0.01 abc	0.018 ± 0.001 bc
	S	20	0.28 ± 0.00 f	0.017 ± 0.000 bc
	NA	5	0.31 ± 0.01 def	0.021 ± 0.001 bc
	NA	10	0.29 ± 0.00 bcdef	0.019 ± 0.001 ab
	NA	15	0.23 ± 0.00 bcdef	0.016 ± 0.001 bc
	NA	20	0.23 ± 0.01 abcd	0.016 ± 0.001 c
	NB	5	0.29 ± 0.01 bcdef	0.019 ± 0.001 bc
	NB	10	0.23 ± 0.00 bcdef	0.018 ± 0.001 bc
	NB	15	0.23 ± 0.01 cdef	0.017 ± 0.001 bc
	NB	20	0.24 ± 0.01 bcdef	0.018 ± 0.001 bc

Note: Values are presented as the mean ± SD followed by different letters that denote the level of significance based on the least significant difference (LSD) test (p < 0.05). CK, control; O, organic fertilizer; S, seedling substrate; NA, nutrient soil A; NB, nutrient soil B.

).

Similarly, the material (M), variety (V), V × M, treatment (T), V × T, and V × M × T significantly affected the dry weight per unit seedling height (Table 1). The S5 treatment significantly increased the dry weight per unit seedling height of Wufengyou 286 by 19.05% compared to the CK. Similarly, the O5 treatment significantly increased the dry weight per unit seedling height of Zhongjiazao 17 by 15.00% compared to the CK (Table 4).

4.3. Attributes of the Antioxidant Response

Variety (V), material (M), treatment (T) V × M, V × T, M × T, and V × M × T significantly affected the activities of CAT, SOD, and POD (Table 1). The S5, S15, and S20 treatments significantly increased the CAT activity of Wufengyou286 by 36.58%, 11.71%, and 15.29%, respectively, compared to the CK. In contrast, the NB5 and NB10 treatments significantly reduced the CAT activity of Wufengyou 286 by 69.16% and 19.81%, respectively, compared to the CK. The S10, S15, and S20 treatments significantly increased the SOD activity of Wufengyou 286 by 54.00%, 15.09%, and 23.38%, respectively, compared to the CK. However, the NB5 and NB10 treatments significantly decreased the SOD activity of Wufengyou 286 by 18.93% and 17.85%, respectively, compared to the CK. The NB5 and NB10 treatments also significantly decreased the POD activity of Wufengyou 286 by 43.98% and 44.80%, respectively, compared to the CK. The S5, S10, S15, and S20 treatments significantly increased the CAT activity of Zhongjiazao 17 by 28.63%, 47.84%, 23.02%, and 21.65%, respectively, compared to the CK. In contrast, the O5, O10, O15, and O20 treatments significantly decreased the POD activity of Zhongjiazao 17 by 30.11%, 35.17%, 12.18%, and 23.20%, respectively, compared to the CK (

Table 5. The antioxidant enzyme activity of direct-seeded rice under different cover materials and different cover thickness treatments.

			CAT Activity	SOD Activity	POD Activity
Variety	Material	Treatment	(mmol·min−1·mg−1·FW)	(U·mg−1·FW)	(U·min−1·g−1·FW)
Wufengyou 286	CK	0	431.52 ± 4.94 d	0.79 ± 0.09 d	259.88 ± 1.67 cd
	O	5	410.45 ± 12.53 de	0.77 ± 0.02 d	274.07 ± 2.55 b
	O	10	416.03 ± 14.62 de	0.77 ± 0.02 d	274.00 ± 1.28 b
	O	15	375.98 ± 12.40 efg	0.77 ± 0.00 d	166.83 ± 7.67 f
	O	20	499.38 ± 12.45 c	0.58 ± 0.04 e	281.08 ± 4.11 b
	S	5	589.39 ± 12.42 b	0.76 ± 0.02 d	269.66 ± 1.43 bc
	S	10	408.40 ± 12.47 de	1.22 ± 0.01 a	298.67 ± 4.32 a
	S	15	482.05 ± 28.93 c	0.91 ± 0.05 bc	253.71 ± 3.96 d
	S	20	497.51 ± 12.40 c	0.97 ± 0.03 b	281.70 ± 2.98 b
	NA	5	210.71 ± 18.77 h	0.64 ± 0.03 e	137.85 ± 6.62 h
	NA	10	355.29 ± 7.15 fg	1.21 ± 0.00 a	305.74 ± 3.99 a
	NA	15	641.41 ± 8.87 a	0.76 ± 0.08 d	307.92 ± 5.75 a
	NA	20	398.22 ± 7.20 de	0.79 ± 0.02 d	198.67 ± 7.01 e
	NB	5	133.09 ± 12.42 i	0.64 ± 0.03 e	145.57 ± 2.86 gh
	NB	10	346.03 ± 12.42 g	0.65 ± 0.04 e	143.46 ± 3.80 h
	NB	15	395.75 ± 18.92 def	0.84 ± 0.02 cd	194.12 ± 6.94 e
	NB	20	565.17 ± 25.91 b	0.78 ± 0.02 d	158.12 ± 0.31 fg
Zhongjiazao 17	CK	0	513.98 ± 26.38 ef	0.55 ± 0.02 de	196.40 ± 11.13 ab
	O	5	292.80 ± 19.14 h	0.99 ± 0.11 a	137.26 ± 3.08 defg
	O	10	293.89 ± 19.21 h	0.99 ± 0.11 a	127.33 ± 2.46 fgh
	O	15	477.26 ± 14.32 f	0.68 ± 0.03 bcd	172.47 ± 3.79 c
	O	20	387.43 ± 14.69 g	0.70 ± 0.04 bc	150.84 ± 5.04 d
	S	5	661.13 ± 19.23 cd	0.69 ± 0.02 bc	187.35 ± 3.87 bc
	S	10	759.88 ± 7.15 a	0.77 ± 0.03 b	208.03 ± 1.08 a
	S	15	632.32 ± 19.04 d	0.36 ± 0.07 fg	144.89 ± 1.57 de
	S	20	625.26 ± 28.01 d	0.57 ± 0.03 cde	185.26 ± 11.78 bc
	NA	5	687.50 ± 21.73 bc	0.63 ± 0.03 cd	141.78 ± 6.94 def
	NA	10	533.87 ± 17.74 e	0.26 ± 0.03 g	131.04 ± 3.35 efgh
	NA	15	658.73 ± 7.15 cd	0.47 ± 0.05 ef	132.06 ± 2.44 efgh
	NA	20	397.23 ± 18.99 g	0.97 ± 0.02 a	142.98 ± 4.75 def
	NB	5	385.50 ± 14.62 g	0.70 ± 0.04 bc	119.07 ± 6.73 h
	NB	10	740.57 ± 21.48 ab	0.59 ± 0.01 cde	180.20 ± 2.93 c
	NB	15	743.35 ± 21.56 a	0.59 ± 0.01 cde	178.89 ± 6.80 c
	NB	20	383.58 ± 14.54 g	0.70 ± 0.04 bc	124.09 ± 1.08 gh

Note: Values are presented as the mean ± SD, followed by different letters that denote the level of significance based on the least significant difference (LSD) test (p < 0.05). CK, control; O, organic fertilizer; S, seedling substrate; NA, nutrient soil A; NB, nutrient soil B.

).

M, V × M, T, V × T, M × T, and V × M × T also significantly affected the contents of MDA, H₂O₂, soluble protein, and ascorbic acid (Table 1). All the treatments of NB significantly decreased the MDA content of Wufengyou 286 by 17.95%, 14.01%, 39.99%, and 47.82%, respectively, compared to the CK. However, NB in all the treatments led to a significant decrease in the contents of H₂O₂ and soluble protein. S in all the treatments significantly decreased the MDA contents of Zhongjiazao 17 compared to the CK. However, O10, O15, and O20 significantly increased the MDA content of Zhongjiazao17 by 84.16%, 24.66%, and 20.93%, respectively, compared to the CK. In the same line, S5, S10, and S20 significantly increased the soluble protein content by 54.32%, 68.36%, and 45.84%, respectively, compared to the CK (

Table 6. The MDA, H₂O₂, soluble protein, and ascorbic acid contents of the direct-seeded rice under different cover materials and cover thickness treatments.

Variety	Material	Treatment	MDA Contents	H2O2 Contents	Soluble Protein Contents	Ascorbic Acid Contents
			(μmol·g−1·FW)	(μmol·g−1·FW)	(mg·g−1·FW)	(μg·g−1·FW)
Wufengyou 286	CK	0	22.84 ± 0.62 c	6.63 ± 0.16 d	7.98 ± 0.17 ab	0.63 ± 0.01 abc
	O	5	19.09 ± 0.67 efg	5.26 ± 0.21 h	8.21 ± 0.69 a	0.63 ± 0.01 abc
	O	10	16.61 ± 0.33 ghi	5.53 ± 0.10 gh	8.52 ± 0.28 a	0.62 ± 0.04 abcd
	O	15	15.89 ± 0.58 hi	5.63 ± 0.16 fgh	2.43 ± 0.11 f	0.56 ± 0.05 d
	O	20	38.47 ± 0.52 b	11.89 ± 0.23 a	4.50 ± 0.05 e	0.67 ± 0.01 a
	S	5	17.95 ± 1.84 fgh	6.00 ± 0.00 efg	8.33 ± 0.39 a	0.62 ± 0.02 abcd
	S	10	50.99 ± 1.46 a	7.82 ± 0.37 c	7.35 ± 0.20 bc	0.60 ± 0.02 abcd
	S	15	17.29 ± 0.69 fgh	6.17 ± 0.16 def	7.05 ± 0.21 cd	0.59 ± 0.02 bcd
	S	20	22.55 ± 1.67 cd	6.23 ± 0.10 de	8.10 ± 0.14 a	0.67 ± 0.02 a
	NA	5	11.48 ± 1.14 j	5.64 ± 0.06 fgh	4.90 ± 0.22 e	0.43 ± 0.01 e
	NA	10	20.14 ± 1.37 cdef	8.20 ± 0.24 c	7.10 ± 0.10 cd	0.58 ± 0.02 cd
	NA	15	21.31 ± 1.73 cde	6.18 ± 0.31 def	8.18 ± 0.32 a	0.59 ± 0.02 bcd
	NA	20	19.75 ± 0.40 def	11.31 ± 0.14 b	6.59 ± 0.16 d	0.65 ± 0.01 abc
	NB	5	18.74 ± 0.95 efgh	5.45 ± 0.12 gh	2.93 ± 0.18 f	0.43 ± 0.01 e
	NB	10	19.64 ± 1.08 defg	5.33 ± 0.12 h	2.80 ± 0.11 f	0.44 ± 0.04 e
	NB	15	13.71 ± 0.27 ij	5.56 ± 0.32 gh	4.56 ± 0.22 e	0.58 ± 0.04 bcd
	NB	20	11.92 ± 0.91 j	5.50 ± 0.14 gh	6.42 ± 0.15 d	0.65 ± 0.02 ab
Zhongjiazao 17	CK	0	18.30 ± 0.92 de	6.60 ± 0.41 bc	4.94 ± 0.62 c	0.58 ± 0.05 a
	O	5	10.72 ± 0.42 h	5.63 ± 0.23 de	1.88 ± 0.02 f	0.41 ± 0.05 f
	O	10	33.69 ± 4.14 a	6.32 ± 0.12 bcde	2.48 ± 0.04 ef	0.43 ± 0.00 ef
	O	15	22.81 ± 0.19 c	5.93 ± 0.15 cde	4.77 ± 0.24 c	0.44 ± 0.02 def
	O	20	22.12 ± 0.43 c	6.73 ± 0.36 bc	7.27 ± 0.21 b	0.59 ± 0.00 a
	S	5	14.82 ± 0.87 fg	6.33 ± 0.27 bcde	7.62 ± 0.19 ab	0.58 ± 0.05 a
	S	10	14.12 ± 1.05 fg	6.34 ± 0.57 bcde	8.32 ± 0.13 a	0.58 ± 0.05 a
	S	15	10.64 ± 0.11 h	6.02 ± 0.36 cde	3.76 ± 0.29 d	0.38 ± 0.03 f
	S	20	12.91 ± 0.50 gh	5.93 ± 0.29 cde	7.20 ± 0.15 b	0.46 ± 0.02 bcdef
	NA	5	18.45 ± 0.40 de	6.36 ± 0.12 bcde	5.36 ± 0.11 c	0.55 ± 0.05 ab
	NA	10	15.26 ± 0.82 efg	6.60 ± 0.25 bc	1.99 ± 0.10 f	0.52 ± 0.02 abcd
	NA	15	16.87 ± 0.40 def	5.56 ± 0.23 e	3.90 ± 0.22 d	0.59 ± 0.02 a
	NA	20	30.56 ± 0.84 ab	6.90 ± 0.27 b	3.00 ± 0.03 e	0.53 ± 0.02 abc
	NB	5	28.02 ± 0.73 b	8.03 ± 0.24 a	7.39 ± 0.03 b	0.50 ± 0.02 abcde
	NB	10	18.66 ± 0.57 d	6.49 ± 0.49 bc	5.03 ± 0.39 c	0.44 ± 0.01 cdef
	NB	15	22.16 ± 0.46 c	6.01 ± 0.17 cde	4.90 ± 0.52 c	0.44 ± 0.02 cdef
	NB	20	16.17 ± 0.41 defg	6.47 ± 0.06 bcd	7.12 ± 0.03 b	0.46 ± 0.01 cdef

Note: Values are presented as the mean ± SD, followed by different letters that denote the level of significance based on the least significant difference (LSD) test (p < 0.05). CK, control; O, organic fertilizer; S, seedling substrate; NA, nutrient soil A; NB, nutrient soil B; MDA, malondialdehyde; H₂O₂, hydrogen peroxide.

).

4.4. Photosynthetic Pigments

The V (variety), M (material), V × M, T, V × T, M × T, and V × M × T significantly affected the contents of chlorophyll and carotenoids (Table 1). All the treatments significantly reduced the chlorophyll content of Wufengyou 286 compared to the CK. In contrast, S5 and NA5 significantly increased the total chlorophyll content of Zhongjiazao 17 by 19.99% and 30.41%, respectively, compared to the CK. Notably, the effect of this increase was the same for the contents of chlorophyll a and chlorophyll b. All the treatments decreased the chlorophyll a to chlorophyll b ratio in Wufengyou 286 but increased the ratio in Zhongjiazao 17. All the treatments also decreased the carotenoid contents of Wufengyou 286. However, the O20, S10, and NA15 treatments significantly increased the carotenoid contents of Wufengyou 286 by 29.94%, 42.94%, and 67.80%, respectively, compared to the CK (

Table 7. The contents of chlorophyll and carotenoids of the direct-seeded rice under different cover materials and different cover thickness treatments.

			Chlorophyll a Contents	Chlorophyll b Contents	Carotenoid Contents	Chlorophyll Contents	Chlorophyll a:b
Variety	Material	Treatment	(mg·g−1·FW)	(mg·g−1·FW)	(mg·g−1·FW)	(mg·g−1·FW)
Wufengyou 286	CK	0	0.40 ± 0.012 ab	0.17 ± 0.004 ab	0.05 ± 0.003 bc	0.57 ± 0.016 ab	2.32 ± 0.024 bcd
	O	5	0.39 ± 0.012 abcd	0.18 ± 0.004 a	0.05 ± 0.002 bc	0.57 ± 0.016 ab	2.14 ± 0.016 e
	O	10	0.28 ± 0.005 hi	0.14 ± 0.002 def	0.04 ± 0.000 cdefg	0.43 ± 0.008 gh	2.01 ± 0.005 f
	O	15	0.28 ± 0.008 hi	0.13 ± 0.003 fg	0.03 ± 0.002 fg	0.41 ± 0.010 h	2.24 ± 0.021 bcde
	O	20	0.27 ± 0.023 i	0.13 ± 0.008 fg	0.04 ± 0.005 defg	0.40 ± 0.030 h	2.13 ± 0.056 ef
	S	5	0.36 ± 0.010 bcdef	0.16 ± 0.003 bc	0.04 ± 0.003 cde	0.52 ± 0.013 bcde	2.26 ± 0.022 bcde
	S	10	0.30 ± 0.020 ghi	0.14 ± 0.007 efg	0.04 ± 0.004 defg	0.44 ± 0.027 fgh	2.22 ± 0.053 cde
	S	15	0.32 ± 0.017 fghi	0.14 ± 0.005 efg	0.04 ± 0.005 cdef	0.45 ± 0.022 fgh	2.32 ± 0.066 bcd
	S	20	0.32 ± 0.008 fgh	0.14 ± 0.003 efg	0.04 ± 0.002 cde	0.46 ± 0.011 efgh	2.34 ± 0.021 bc
	NA	5	0.39 ± 0.013 abc	0.17 ± 0.005 abc	0.05 ± 0.003 bc	0.56 ± 0.019 abc	2.34 ± 0.024 bc
	NA	10	0.28 ± 0.043 hi	0.13 ± 0.012 g	0.03 ± 0.009 g	0.41 ± 0.055 h	2.20 ± 0.135 de
	NA	15	0.43 ± 0.013 a	0.17 ± 0.003 ab	0.06 ± 0.002 a	0.60 ± 0.017 a	2.49 ± 0.031 a
	NA	20	0.35 ± 0.017 cdefg	0.15 ± 0.006 cd	0.05 ± 0.004 cd	0.50 ± 0.023 cdef	2.25 ± 0.034 bcde
	NB	5	0.34 ± 0.011 defg	0.15 ± 0.006 cd	0.04 ± 0.001 cde	0.50 ± 0.016 def	2.21 ± 0.014 cde
	NB	10	0.34 ± 0.008 efg	0.14 ± 0.003 de	0.04 ± 0.002 cde	0.48 ± 0.011 defg	2.36 ± 0.021 b
	NB	15	0.31 ± 0.006 ghi	0.13 ± 0.002 efg	0.03 ± 0.001 efg	0.44 ± 0.007 fgh	2.28 ± 0.034 bcd
	NB	20	0.37 ± 0.008 bcde	0.16 ± 0.002 bc	0.06 ± 0.002 ab	0.53 ± 0.010 bcd	2.30 ± 0.032 bcd
Zhongjiazao 17	CK	0	0.34 ± 0.010 efgh	0.16 ± 0.004 defg	0.04 ± 0.002 fghi	0.50 ± 0.014 cde	2.19 ± 0.008 defg
	O	5	0.29 ± 0.011 i	0.14 ± 0.002 gh	0.04 ± 0.004 i	0.43 ± 0.012 f	2.10 ± 0.077 efg
	O	10	0.31 ± 0.031 hi	0.15 ± 0.010 efgh	0.04 ± 0.005 hi	0.46 ± 0.041 ef	2.06 ± 0.078 fg
	O	15	0.31 ± 0.016 hi	0.14 ± 0.004 h	0.04 ± 0.004 ghi	0.44 ± 0.020 ef	2.25 ± 0.067 cde
	O	20	0.40 ± 0.013 bcd	0.19 ± 0.015 ab	0.06 ± 0.001 cd	0.59 ± 0.028 ab	2.21 ± 0.101 def
	S	5	0.42 ± 0.010 abc	0.17 ± 0.002 abc	0.06 ± 0.004 bcd	0.60 ± 0.012 ab	2.41 ± 0.029 ab
	S	10	0.38 ± 0.013 cdef	0.16 ± 0.007 cde	0.06 ± 0.003 bc	0.54 ± 0.019 bc	2.30 ± 0.055 abcd
	S	15	0.35 ± 0.010 efgh	0.15 ± 0.003 fgh	0.05 ± 0.002 def	0.49 ± 0.012 cdef	2.38 ± 0.023 abc
	S	20	0.32 ± 0.013 hi	0.14 ± 0.005 gh	0.05 ± 0.003 efgh	0.46 ± 0.017 ef	2.26 ± 0.040 bcde
	NA	5	0.46 ± 0.011 a	0.19 ± 0.003 a	0.07 ± 0.003 ab	0.65 ± 0.015 a	2.45 ± 0.017 a
	NA	10	0.34 ± 0.027 efgh	0.15 ± 0.010 efgh	0.05 ± 0.005 defg	0.49 ± 0.037 cdef	2.28 ± 0.049 bcd
	NA	15	0.42 ± 0.021 ab	0.17 ± 0.004 abcd	0.07 ± 0.005 a	0.60 ± 0.025 ab	2.46 ± 0.066 a
	NA	20	0.38 ± 0.011 bcde	0.17 ± 0.005 bcd	0.06 ± 0.002 cde	0.55 ± 0.016 bc	2.24 ± 0.045 cde
	NB	5	0.37 ± 0.008 defg	0.16 ± 0.003 cdef	0.05 ± 0.002 defg	0.53 ± 0.011 cd	2.31 ± 0.018 abcd
	NB	10	0.33 ± 0.017 fghi	0.14 ± 0.004 gh	0.05 ± 0.003 defg	0.48 ± 0.020 def	2.35 ± 0.075 abcd
	NB	15	0.33 ± 0.010 ghi	0.15 ± 0.005 efgh	0.05 ± 0.002 fghi	0.48 ± 0.013 def	2.25 ± 0.082 cde
	NB	20	0.32 ± 0.015 hi	0.15 ± 0.004 defg	0.04 ± 0.003 fghi	0.47 ± 0.019 def	2.04 ± 0.042 g

Note: Values are presented as the mean ± SD, followed by different letters that denote the level of significance based on the least significant difference (LSD) test (p < 0.05). CK, control; O, organic fertilizer; S, seedling substrate; NA, nutrient soil A; NB, nutrient soil B.

).

4.5. Correlation Analysis

The plant height was significantly positively correlated with the leaf age, dry weight, germination rate, emergence rate, and POD activity. The germination rate was significantly positively correlated with the POD activity. Moreover, the dry weight per unit seedling height significantly positively correlated with the contents of chlorophyll b and total chlorophyll. However, the activities of SOD and POD significantly negatively correlated with the content of carotenoids (Figure 2).

5. Discussion

The direct-seeding of rice is increasingly being adopted by farmers because it is simple and saves labor and time [2]. The inherent complexities of the direct-seeding methodologies limit the establishment of uniform rice seedling growth, which is paramount for the maturation and productivity of DSR [7].

Appropriate high sowing depths or cover thickness enhance the germination and seedling establishment of rice seeds. However, deeper sowing depths may also limit germination and the establishment of seedlings [11]. In this study, the germination rates of the uncovered seeds of both varieties ranged between 50% and 60%. However, the germination rate increased to between 70% and 80% when the seeds were covered with 5 mm of the various materials. Of note, the seedling emergence rate of both varieties tended to decrease as the thickness of the different cover materials increased. This finding was consistent with that of a previous study. This phenomenon was attributed to covering material that was too thick, which limited the sufficient utilization of oxygen by the seeds; this affected germination and seedling formation [21]. This finding strongly suggested that an appropriate covering material thickness was a prerequisite to improve the germination and rate of emergence of the DSR.

In this study, the covering treatments increased the plant height in both varieties, which increased further with the increasing thickness of the cover material. Previous studies hypothesized that there was a significant increase in the contents of growth hormones and cytokinin during the seed breakthroughs [22]. Herein, there was a significant increase in leaf age and plant height with an increase in the thickness of the cover treatments, possibly because the thicker cover led to higher contents of the relevant hormones. Low oxygen stress induces an increase in the contents of ethylene and abscisic acid (ABA) in the root system, which inhibits the transport of aboveground indole acetic acid (IAA) to the roots, which leads to the accumulation of IAA in the aboveground organs [23]. In this study, the accumulation of IAA at the base of the stem promoted the elongation of stems in the covered treatments, which could potentially trigger short-term hypoxic stress and induce an increase in the height of rice seedlings after the treatment. Moreover, an increase in the thickness of the cover material prolonged the time of emergence of the rice seedlings, which led to a reduction in the leaf age and accumulation of the dry weight of the seedlings. These findings were consistent with the results of previous studies [11]. The reduction in leaf age and biomass was attributed to the low oxygen environment created by the covering, which limits root vigor and the respiration rate. This limitation leads to a decrease in the uptake of nutrients by the root system, thus, inhibiting the formation of tillers and the accumulation of biomass [24].

Reactive oxygen species (ROS) include substances, such as superoxide and peroxides, which create a chain reaction with unsaturated fatty acids in the cell membranes; thus, causing damage to the plant cells because the reactants destabilize the stable shape of the cell membranes [25]. Increasing the activity of antioxidant enzymes, including CAT, SOD, and POD, play an important role in suppressing extra ROS, thereby maintaining normal levels of these ROS [26]. Previous studies hypothesized that the activities of SOD and POD of the seedlings under the straw seedling board treated with the seedling substrate are higher than those of seedlings under natural soil treatment [27]. Herein, the physiological indicators of the Wufengyou 286 seedlings suggested that the respective activities of SOD and POD increased after treatment with the seedling substrate. Similarly, the CAT activity for Zhongjiazao 17 under the seedling substrate treatment was higher than that of the CK plants. These findings strongly suggested that the seedling substrates regulate the composition of the antioxidant system of rice seedlings [26]. The high activity of antioxidant enzymes in the rice seedlings under the seedling substrate treatment can be attributed to the enhancement of photosynthesis, biomass, and leaf age, which enhance the potential of directly seeded rice to adjust to an exposed growth environment.

ROS induce lipid peroxidation, which leads to the production of MDA, which can also aggravate the damage to the cell membranes [28]. Chen et al. reported a significant decrease in the MDA content in mechanically direct-seeding treatment groups [1]. In this study, the MDA content of Zhongjiazao 17 under all the substate treatments was lower than that of CK plants. Hydrogen peroxide (H₂O₂) is a type of ROS that is widely produced in many biological systems and is considered to be an important signaling molecule that mediates various physiological and biochemical processes in plants. The generation of H₂O₂ in plant cells results from normal metabolism from various sources [29]. Previous studies discovered an integrated regulatory network in which miR398b enhances the total SOD activity, which leads to an increase in the content of H₂O₂. Consequently, this improved disease resistance. Similarly, there was an increase in SOD activity and the content of H₂O₂ in the Wufengyou 286 seedlings under the seedling substrate 10 treatment compared to those under the CK treatment [30].

Soluble proteins act as osmoregulatory substances in plants, thereby maintaining the integrity of the cell structure and function under stress conditions [20]. Ascorbic acid is one of the most abundant water-soluble antioxidants in plants. It acts as a major redox buffer and regulates various physiological processes that control plant growth, development, and responses to stress [31]. The direct-seeding of rice results in a more active antioxidant stress response [1], which requires the enhanced osmoregulatory and antioxidant responses of the seedlings to adapt to the exposed growing conditions. Herein, the cover treatments of different materials significantly regulated the contents of soluble protein and ascorbic acid of the rice seedlings, which underscores their potential to improve the ability of rice to survive under adverse conditions by regulating their osmotic stability.

Chlorophyll is the primary pigment in plant photosynthesis and is responsible for transforming light energy into chemical energy and regulating various biochemical processes within plants [32]. Carotenoids serve a multitude of important functions in plants and are, thus, indispensable. They participate in photosynthesis, provide photoprotection, contribute to pigmentation, aid in phytohormone synthesis, and facilitate signaling [33]. In this study, the seedling substrate and nutrient soil A treatments significantly and partially increased the contents of chlorophyll and carotenoids in the seedlings compared to the CK plants. This increase was attributed to the nutrient soil and seedling substrate, which provided essential nutrients for the early growth of the rice seedlings by enhancing the biosynthesis of photosynthetic pigments. Previous studies postulated that the application of phosphorus and organic carbon favors photosynthesis, stomatal conductance, water use efficiency, and increased leaf water potential in rice [34].

Overall, treatment with optimized covering materials enhances the early growth of DSR by improving the morphological attributes and regulating the physio-biochemical responses (Figure 3).

6. Conclusions

An appropriate cover treatment enhances the early growth of the direct-seeded rice by improving its morphological attributes, such as plant height and dry weight, and regulating the physio-biochemical responses, such as antioxidants and photosynthetic pigments. The rate of seedling growth of both varieties decreased as the thickness of the different cover materials increased. Comparatively, using the organic fertilizer that covers the seedling substrate in the 5 mm treatments is the most effective for promoting early rice growth. This study elucidated the effects of different covering materials on the early growth of direct-seeded rice to further guide their actual production. However, the selection and matching of different covering materials and covering thicknesses require more in-depth research.