1. Introduction
Wheat is one of the primary grain crops throughout the world; however, fungal diseases provide a great obstacle to improving wheat yields, now and into the future [
1]. Fusarium head blight not only reduces the yield of wheat but also produces toxins harmful to the human body [
2]. Wheat powdery mildew is a frequently encountered foliar disease that causes serious yield losses [
3]. However, chemical control is still the most effective way to prevent bacteria epidemics [
1].
At present, the commonly used pesticide application equipment (PAE) in China mainly includes knapsack sprayers, boom sprayers, and plant protection unmanned aircraft vehicles (UAVs) [
4]. On small Asian farms ranging from one to two hectares, boom sprayers, and knapsack sprayers have been widely used due to their simple operation and relatively low price [
5,
6]. Due to the flexibility, ease of operation, efficiency, accuracy, and penetration advantage of the rotor wind field of UAV [
7], agricultural UAVs have developed rapidly [
8,
9]. Although agricultural UAVs have been used in remote sensing monitoring, seeding, fertilization, auxiliary pollination [
10], and other fields, their main usage is pesticide application [
11].
The spraying effects of different models of UAVs vary greatly [
12,
13]. Different brands and product models of UAVs have different sizes, load limits, rotor numbers and shapes, nozzle atomization principles [
14], and nozzle layouts [
15]. Many scholars have studied the crop-canopy deposition of different types of UAVs in rice, maize, wheat, and trees and have optimized the operation parameters, such as flight height and flight speed [
16,
17,
18,
19,
20]. Zhang et al. [
21] compared two typical kinds of UAV models based on the first industry standard of China and proved that the decrease of flight speed and flight height in a certain range can increase the effective spray width and droplet penetration rate. Wang et al. [
22] tested the spraying coverage and work efficiency of four typical UAV models; the results showed that the UAV models had uneven droplet distribution and low time utilization. Chen et al. [
23] studied the deposition of oil-powered single-rotor UAVs and electric single-rotor UAVs in the rice canopy; the results showed that, compared with the knapsack manual sprayer, the UAVs improved the penetration rate. Wang et al. [
24] compared the droplet deposition, control efficacy, and working efficiency of a six-rotor UAV with a self-propelled boom sprayer and two conventional knapsack sprayers on wheat crop. It was found that the deposition uniformity and droplet penetrability of the UAVs were poor; however, the UAVs had comparable control efficacy on wheat aphids and had high work efficiency.
The above studies mainly focused on the optimization of UAVs’ operation parameters. In terms of performance evaluation, on the one hand, the UAVs’ model and assessment indicators were not comprehensive; on the other hand, the inconsistent experimental conditions of different kinds of literature, such as moderate meteorological conditions, crop varieties, and disease indexes, resulted in significant differences in the experimental results. With the improvement and upgrading of UAVs [
13], the continuous tracking of their operational performance plays a positive role in promoting the benign development of UAVs. In this paper, three typical UAVs were compared with a self-propelled boom sprayer and a knapsack electric sprayer. The droplet coverage and penetration rate, pesticide-mixture utilization rate, labor productivity, and control efficacy of wheat powdery mildew and Fusarium head blight were investigated; based on these indicators, a comprehensive performance evaluation method for PAE was proposed. The differences in droplet deposition and pesticide-mixture utilization rate between UAVs and the ground equipment were analyzed by statistics methods. This study aims to comprehensively evaluate the performance of UAVs in wheat disease control, compared with a boom sprayer and knapsack electric sprayer; this study points out the operational advantages of UAVs and discusses the direction for improvement.
2. Materials and Methods
2.1. Pesticide Application Equipment
The PAE used in the experiment includes an XP2020 electric four-rotor UAV (Ji Fei Technology Co., Ltd., Guangzhou, China), a T16 electric six-rotor UAV (DJI Innovation Technology Co., Ltd., Shenzhen, China), a CE20 electric single-rotor UAV (Wuxi Hanhe Aviation Technology Co., Ltd., Jiangsu, China), a 3WPZ-700 self-propelled boom sprayer (Qingzhou Wolong Pesticide Application Equipment Co., Ltd., Shandong, China), and a 3WBD-18 knapsack electric sprayer (Taizhou Luqiao Minghui Electric Sprayer Co., Ltd., Zhejiang, China).
The main technical parameters of each machine used in this test are shown in
Table 1 and the selection of technical parameters was based on the manufacturer’s recommendation. The XP2020 electric four-rotor UAV and T16 electric six-rotor UAV were equipped with variable control systems and the flow rate changed with the changes in flight speed and spray width, thus ensuring the stability of the spray volume. However, the CE20 electric single-rotor UAV was not equipped with the variable control system; the tested spray volume fluctuated in the range of 13.5–15 L·ha
−1. The operation sites of the different machines are shown in
Figure 1.
2.2. Test Material
The plant protection operation quality evaluation system, with a horizontal positioning accuracy of 1.1 cm and elevation positioning accuracy of 1 cm (Nanjing Institute of Agricultural Mechanization, Ministry of Agriculture and Rural Affairs of China), was used to collect the flight trajectory of the UAVs and locate sampling points. Meteorological conditions were observed via the Watchdog 2900ET ground weather station, with a minimum sampling interval of 1 min (Spectrum Technologies., Inc., Aurora, IL, USA). Canopy density was tested by the LAI-2200C Canopy Analyzer (Beijing Ligotai Technology Co., Ltd., Beijing, China). The spray deposition amount was measured by the 722N Visible Light Spectrophotometer (Shanghai Yidian Analytical Instrument Co., Ltd., Shanghai, China); polyester card (ϕ 9 cm), 0.22 μm syringe driven filter (Jinteng PES filter, Tianjin, China); allure red AC, purity 85%, (Beijing Solarbio Technology Co., Ltd., Beijing, China). The sampling equipment for droplet deposition included a 9000F mark ii Scanner (Canon, Tokyo, Japan) and water-sensitive paper, size 76 mm × 26 mm, (Syngenta, Basel, Switzerland). Other test materials included universal clamps for clamping water-sensitive paper and polyester cards, plastic bags for collecting water-sensitive paper and polyester cards, scissors for cutting off wheat plants, and electronic balance, precision 0.01 g, range 200 g, electronic balance, precision 0.1 g, range 30 kg.
2.3. Wheat Growth Condition and Meteorological Conditions
The test was conducted in a national modern agriculture demonstration zone in Jianhu County, Jiangsu Province, China. The wheat variety was Xumai 33. The wheat growth and meteorological conditions regarding the two experiments are shown in
Table 2.
The LAI-2200C canopy analyzer was used to measure the leaf-area index. Three randomly selected sites of wheat plants with a 0.09 m2 area were cut off and weighed and the average plant mass per unit area was obtained.
The two tests were conducted from 4 pm to 6 pm, the wind speed was within 0.5 m·s−1, the wind direction was perpendicular to the UAV flight path, the temperature was 19–21 °C, and the humidity was 75–81%. The meteorological conditions were very favorable for aerial spraying.
2.4. Spray Pesticides and Reagents
The spray pesticides were suspended agents, including tebuconazole (a prochloraz compound pesticide), difenoconazole (an azoxystrobin compound pesticide), and imidacloprid; they were applied to control wheat powdery mildew, Fusarium head blight, and aphids. The spraying agent was prepared according to the spray area and the spray volume of equipment applied. A certain amount of allure red AC was added to prepare the tracer solution [
25] with a concentration of 5 g·L
−1. Approximately 20 mL of spray solution was taken back to the laboratory to analyze the concentration of the allure red AC. After the spray solution was diluted to a certain multiple, the diluted spray liquid was filtered by a 0.22 μm syringe-driven filter. We tested the absorbance of the liquid before and after filtration in the laboratory and verified that the 0.22 μm syringe-driven filter could effectively block suspended pesticides and dust particles but had no effect on the concentration of allure red AC. The absorbance was determined by a visible light spectrophotometer at a wavelength of 504 nm and the concentration of allure red AC in the spray solution was analyzed.
2.5. Experiment Design
2.5.1. Data Sampling
The sampling method for the droplet deposition test in the canopy is shown in
Figure 2. One piece of water-sensitive paper and one piece of polyester card were placed on the top of the canopy to acquire the droplet deposition. Another piece of water-sensitive paper was placed in the middle layer to test droplet penetration and another polyester card was placed on the bottom layer to capture the pesticide loss rate [
26]. After the placement of the sampling materials was completed, we stirred the wheat plant to restore its normal state to ensure the accuracy of the sampling.
The flight path and sampling point position of the XP2020 electric four-rotor UAV is shown in
Figure 3. The droplet deposition test of the UAV was repeated four times while the droplet deposition test of the self-propelled boom sprayer and the knapsack electric sprayer was sampled once. Each test area was set with 9 sampling points. The GPS information of the 9 sampling points was recorded by the plant protection operation quality evaluation system. The plant protection operation quality evaluation system was installed on the top of the UAV with less interference to keep the antenna transmission signal stable. When the spray operated normally, the plant protection operation quality evaluation system tracked the GPS information of the sprayer’s operation track in real-time and reflected it directly on the mobile terminal through the 4G module. The status information was collected and uploaded synchronously and the data were exported after the completion of the operation [
27]. According to the GPS information collected by the plant protection operation quality evaluation system, the operation time and the actual operation area of the sprayer were obtained through the software [
28]. After spraying, the actual spraying amount and spray area were recorded.
2.5.2. Droplet Deposition and Penetration
The deposition amount and penetration of the droplets in the canopy reflect the operation quality of the spray. For the prevention and control of plant diseases and insect pests in the middle and lower parts of plants, the droplets were required to have high penetration. Droplet deposition data were collected by water-sensitive paper and then processed by image analysis software. Water-sensitive papers were scanned and saved as a picture with a resolution of no less than 600 dpi. The droplet coverage rate was analyzed with Deposit Scan software [
29]. The penetration rate was the ratio of the middle-layer coverage rate to the upper-layer coverage rate.
2.5.3. Pesticide-Mixture Utilization Rate
The pesticide-mixture utilization rate refers to the ratio of the amount of pesticides deposited per unit area and the spray amount per unit area. The higher the pesticide-mixture utilization rate, the less droplet loss there was in the air or on the ground. Crop deposition was measured by using tracers and manual collection cards or plant sampling. Allure red AC was used as a tracer due to its high recovery rate and strong light stability. Polyester card was used as a manual collection card due to its high elution rate.
The polyester cards were taken back and sealed to be stored separately after spraying. About 15 wheat plants around the sampling site were clipped, sealed, and light-shielded for storage [
30]. The polyester cards were eluted with 15 mL of water and the wheat plant was timely weighed and eluted with 100 mL of water. The eluent of the polyester card, eluent of the sample wheat plant, and eluent of the blank wheat plant were filtered by a 0.22 μm syringe-driven filter. The absorbance was determined by the visible light spectrophotometer and the concentration of allure red AC in the eluent was analyzed.
The spray deposition amount per unit area of the polyester card was calculated according to Equation (1) [
31]; the spray deposition amount per unit area of the wheat plant was calculated according to Equation (2):
where,
βdep is the spray deposition amount per unit area, mL·cm
−2;
ρsmpl is the sample eluent concentration, mg·L
−1;
ρblk is the blank sample eluent concentration, mg·L
−1;
Vdii is the volume of eluent, mL;
ρspray is the concentration of allure red AC spray liquid, mg·L
−1;
Acol is the area of the polyester card, cm
2;
m is the weight of the collected plant samples, g; and
Sm is the plant weight per unit area, g·cm
−2.
The spray deposition rate was shown as Equation (3), which was obtained by the ratio of the amount of pesticides deposited per unit area and the spray amount per unit area:
where,
βdep% is the spray deposition rate, %;
βdep is the deposition amount per unit area, mL·cm
−2; and
βV is the spray amount per unit area, L·ha
−1.
The pesticide-mixture utilization rate of the wheat plant was the spray deposition rate of the wheat plant. However, the pesticide-mixture utilization rate of the polyester card refers to the deposition rate tested by the top polyester card minus the pesticide loss rate tested by the bottom polyester card [
26].
2.5.4. Labor Productivity
Labor productivity refers to the spraying area of a person in a unit of time. In order to reduce the time consumption caused by turning around and the acceleration/deceleration of PAE, the area of the production efficiency assessment should be greater than 2 ha. Labor productivity was calculated according to Equation (4):
where,
G is labor productivity, ha per man-hour;
U is the spraying area, ha;
Tb is the working time, h; and
A is the number of operators.
2.5.5. Control Efficacy of Wheat Powdery Mildew and Fusarium Head Blight
The control efficacy of wheat powdery mildew and Fusarium head blight was investigated 20 days after the second application. Five sites were investigated in each test area. The investigation method of wheat Fusarium head blight control efficacy was that about 500 plants were investigated in each site, which was divided into 5 grades according to the proportion of diseased ear parts compared to the whole ear; the number of ears at each grade was recorded. The investigation method of wheat powdery mildew control efficacy: about 100 leaves of 30–40 plants were investigated at each site. According to the proportion of the diseased leaf area compared to the total leaf area, the severity of powdery mildew was divided into 9 grades, respectively. They were 0%, 1%, 5%, 10%, 20%, 40%, 60%, 80%, and 100% and the number of leaves at each grade was recorded. The disease index of Fusarium head blight was calculated according to Equation (5) and the disease index of powdery mildew was calculated according to Equation (6). The control efficacy was calculated by the difference between the disease index of the blank control area and the treatment area divided by the blank control area disease index [
32,
33]:
where,
I and
J are the index of the wheat powdery mildew and Fusarium head blight disease, respectively, %;
ni and
nj are the corresponding number of diseased ears and leaves at each grade, respectively;
N is the total number of ears or leaves;
i is the wheat Fusarium head blight severity level,
i = 0, 1, 2, 3, 4; and
j is the proportion of the diseased leaf area compared to the total leaf area,
j = 0%, 1%, 5%, 10%, 20%, 40%, 60%, 80%, 100%.
2.5.6. Performance Comprehensive Evaluation of PAE
The performance of the PAE was comprehensively evaluated based on four indicators: penetration rate, pesticide utilization rate, labor productivity, and control efficacy of Fusarium head blight. All four indicators had the same weight factor of 0.25. The comprehensive score of each PAE was calculated according to Equation (7). The closer the score was to 1, the better the performance of the PAE:
where,
sa is the comprehensive score of the PAE
a,
a = XP2020, T16, CE20, 3WPZ-700, 3WBD-18;
k is the evaluation indicator, numbers from 1 to 4 are penetration rate, pesticide utilization rate, labor productivity, and control efficacy of Fusarium head blight, respectively;
pk,a is the value of the
k evaluation indicator of PAE
a; and
pk,max is the maximum value of the
k evaluation indicator of all PAE.
2.5.7. Data Analysis
The statistical analysis software SAS was applied for variance analysis and Duncan’s method for multiple comparative analysis; the coverage rate, penetration rate, and pesticide utilization rate of different PAE were graded. The significant difference between the polyester card method and the plant method was analyzed by a paired t-test and the significant level of α was 0.05.
3. Results
3.1. Droplet Deposition and Penetration
The droplet coverage rate and penetration rate of various sprayers are shown in
Figure 4. The results of the four tests were analyzed by variance analysis and multiple comparison analysis. The 3WPZ-700 self-propelled boom sprayer had the highest upper-layer coverage rate of 57% and it also had the highest penetration rate of 63.9%. The coverage rate of the 3WBD-18 knapsack electric sprayer was the second highest, 24.83%; meanwhile, it had the lowest penetration rate of 12.6%. The coverage rate of the UAVs was the smallest, no more than 8%, and the penetration rate was between 30 and 45%. There was no significant difference in the upper coverage rate and penetration rate of the three UAVs.
3.2. Pesticide-Mixture Utilization Rate
The pesticide-mixture utilization rate results of four tests and the average values detected by the polyester cards of the UAVs are shown in
Figure 5. The results of the four tests were analyzed by variance analysis and multiple comparison analysis. The pesticide-mixture utilization rate of the XP2020 electric four-rotor UAV was stable and at the highest level, which indicated that the sprayer had good deposition uniformity. On the contrary, the pesticide-mixture utilization rate monitor value of the T16 electric six-rotor UAV fluctuated while the pesticide-mixture utilization rate monitor value of the CE20 electric single-rotor UAV was at a relatively low level.
The pesticide deposition rate, loss rate, pesticide-mixture utilization rate detected by the polyester card, and the pesticide-mixture utilization rate detected by the plant are shown in
Table 3. It can be seen from
Table 3 that the XP2020 electric four-rotor UAV had the highest pesticide deposition rate of 85.2% and the highest pesticide-mixture utilization rate, as detected by the polyester card of 75.7%. The 3WPZ-700 self-propelled boom sprayer had the second highest pesticide deposition rate of 83.6%; meanwhile, its pesticide loss rate was the most serious, which was 18.7%, resulting in a pesticide-mixture utilization rate that was 10.8% lower than that of the XP2020 electric four-rotor UAV. In general, the average pesticide-mixture utilization rate of the UAVs was 64.9%, which was similar to that of the boom spray. Meanwhile, the pesticide-mixture utilization rate of the 3WBD-18 knapsack electric sprayer was the lowest, at 27.8%.
The paired
t-test was applied for the mean results of the pesticide-mixture utilization rate, which were obtained by the polyester card method and the wheat plant method; they are shown in
Table 4. The occurrence probability of the original hypothesis was 0.056, which was greater than the significance level
α 0.05, indicating that there was no significant difference between the pesticide-mixture utilization rates detected by the polyester card and plant.
3.3. Labor Productivity
The labor productivity of various machines is shown in
Table 5. The T16 electric six-rotor UAV had the highest labor productivity of 6.88 ha per man-hour. The XP2020 electric four-rotor UAV had the lowest labor productivity of 4.57 ha per man-hour, among the UAVs. The UAVs’ average labor productivity was 5.75 ha per man-hour, which was similar to that of the 3WPZ-700 self-propelled boom sprayer and 21.3 times that of the 3WBD-18 knapsack electric sprayer.
The tank capacity of the 3WPZ-700 self-propelled boom sprayer was about 700 L and the spraying volume was 270 L/ha; a tank of pesticide mixture liquid can spray 2.6 ha. During the labor productivity test, the 3WPZ-700 self-propelled boom sprayer saved the time of the site transition and the filling of the pesticide mixture liquid; its time utilization rate was 100%. For other sprayers, pesticide mixture liquid should be added once more during the spraying operation.
3.4. Control Efficacy of Wheat Powdery Mildew and Fusarium Head Blight
The control efficacies of the wheat powdery mildew and Fusarium head blight of various machines are shown in
Figure 6. The control efficacy of each sprayer on wheat Fusarium head blight was more than 90%. Meanwhile, the control efficacy of each sprayer on wheat powdery mildew was relatively poor, merely between 50 and 70%. In the blank control area, the Fusarium head blight disease index was 9.42% while the wheat powdery mildew disease index was 43.30%.
3.5. Performance Comprehensive Score of PAE
The performance comprehensive scores of the five PAE items: XP2020, T16, CE20, 3WPZ-700, and 3WBD-18 were 0.803, 0.877, 0.755, 0.929, and 0.399, respectively. The closer the score was to 1, the better the performance of the PAE; so, the descending order of the performance comprehensive evaluation was 3WPZ-700 self-propelled boom sprayer, T16 electric six-rotor UAV, XP2020 electric four-rotor UAV, CE20 electric single-rotor UAV, and 3WBD-18 knapsack electric sprayer.
4. Discussion
The descending order of the upper droplet coverage was 3WPZ-700 self-propelled boom sprayer; 3WBD-18 knapsack electric sprayer; and UAVs. This was because the UAV was one of low-volume spray [
19] and the spray volume in this experiment did not exceed 15 L·ha
−1. Therefore, the influence of the droplet coverage rate was not calculated in the performance comprehensive evaluation of the PAE. The descending order of the droplet penetration rate was the 3WPZ-700 self-propelled boom sprayer, UAVs, and 3WBD-18 knapsack electric sprayer. The UAVs improved the penetration rate compared with the knapsack electric sprayer; this was due to airflow from the rotor wind field transporting droplets to the middle and lower layers of crops [
21].
The descending order of pesticide-mixture utilization rate was the XP2020 electric four-rotor UAV; T16 electric six-rotor UAV, 3WPZ-700 self-propelled boom sprayer; CE20 electric single-rotor UAV; and 3WBD-18 knapsack electric sprayer. The XP2020 electric four-rotor UAV had a high pesticide deposition rate and a low pesticide loss rate; so, the pesticide-mixture utilization rate had achieved the highest level. For the T16 electric six-rotor UAV, the nozzle layout [
15] and variable spray control system should be further optimized to increase pesticide deposition uniformity. For the CE20 electric single-rotor UAV, the wind speed of the rotor wind field should be matched with the crop leaf area density [
34,
35] to further improve deposition and reduce pesticide loss. In general, for the UAV spraying with high drift risk [
36], the low wind speed on the test day played a non-negligible role in achieving a high pesticide-mixture utilization rate for the UAV [
37].
The UAVs had achieved comparable results with the boom sprayer in terms of labor productivity [
24]. The average labor productivity of the UAVs was 5.75 ha per man-hour, which was 21.3 times that of the knapsack sprayer.
The control efficacy of each sprayer on wheat Fusarium head blight was more than 90%. However, the control effect of various PAE items on wheat powdery mildew was relatively poor. The possible reason was that the planting density of the wheat was very high and the permeability of the field was poor, resulting in the serious incidence of wheat powdery mildew [
38,
39]. The wheat powdery mildew disease index of the blank control area was 43.30%, which eventually lead to difficult treatment. Therefore, the influence of control efficacy on wheat powdery mildew was not calculated in the performance comprehensive evaluation of PAE.
The average performance comprehensive score of the UAVs was 0.812, which was slightly lower than the score of 0.929 for the 3WPZ-700 self-propelled boom sprayer but much higher than the score of 0.399 for the 3WBD-18 knapsack electric sprayer. In the future, the UAVs should be further optimized, in the aspects of their flight control, nozzle layout [
15], variable spraying control, power, and load matching, to further improve the uniformity of droplet deposition and labor productivity.