Exogenous GR24 Inhibits Strawberry Tillering by Affecting the Phytohormone Signaling and Sugar Metabolism Pathways
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Treatments
2.2. Measurement of Plant Growth Indexes
2.3. Determination of the Endogenous Phytohormone Content
2.4. Determination of Sucrose, Fructose, and Glucose Contents
2.5. Transcriptome Analysis
2.6. Real-Time Fluorescence Quantification (RT-qPCR)
2.7. Statistical Analysis
3. Results
3.1. Effects of Different Concentrations of GR24 on Stem Diameter and Its Growth
3.2. Effects of Different Concentrations of GR24 on Plant Height, Petiole Length, and Their Growth
3.3. Effect of Different Concentrations of GR24 on Leaf Thickness and Its Growth
3.4. Effect of Different Concentrations of GR24 on Strawberry Stolons and Flowering Branches
3.5. Effect of GR24 on the Sugar Content of the Strawberry Crowns
3.6. Effect of GR24 on the Content of Endogenous Phytohormones
3.7. Transcriptome Analysis of the Cytokinin Anabolic and Signaling Pathways
3.8. Transcriptome Analysis of the GA Anabolic and Signaling Pathways
3.9. Transcriptome Analysis of the Auxin Signaling Pathway
3.10. Transcriptome Analysis of the ABA Signaling Pathway
3.11. Transcriptome Analysis of the Sugar Metabolism Pathway
3.12. Real-Time Quantitative PCR of the Key Genes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Peng, Y.; Jiang, Y.; He, C.; She, M.; Li, M.; Chen, Q.; Zhang, Y.; Lin, Y.; Zhang, Y.; Wang, Y.; et al. Exogenous GR24 Inhibits Strawberry Tillering by Affecting the Phytohormone Signaling and Sugar Metabolism Pathways. Agronomy 2023, 13, 3078. https://doi.org/10.3390/agronomy13123078
Peng Y, Jiang Y, He C, She M, Li M, Chen Q, Zhang Y, Lin Y, Zhang Y, Wang Y, et al. Exogenous GR24 Inhibits Strawberry Tillering by Affecting the Phytohormone Signaling and Sugar Metabolism Pathways. Agronomy. 2023; 13(12):3078. https://doi.org/10.3390/agronomy13123078
Chicago/Turabian StylePeng, Yuting, Yuyan Jiang, Caixia He, Musha She, Mengyao Li, Qing Chen, Yong Zhang, Yuanxiu Lin, Yunting Zhang, Yan Wang, and et al. 2023. "Exogenous GR24 Inhibits Strawberry Tillering by Affecting the Phytohormone Signaling and Sugar Metabolism Pathways" Agronomy 13, no. 12: 3078. https://doi.org/10.3390/agronomy13123078