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Article

High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monocultures

1
Department of Crop Sciences, University of Illinois, Urbana, IL 61801, USA
2
Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS, UNS-CONICET), Departamento de Agronomía, Universidad Nacional del Sur, Bahía Blanca B8000, Argentina
3
Laboratorio de Biodiversidad Vegetal y Microbiana, Instituto de Investigaciones en Ciencias Agrarias Rosario (IICAR-CONICET), Universidad Nacional de Rosario, Zavalla S2000, Argentina
4
Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Weijian Zhang
Agronomy 2022, 12(4), 954; https://doi.org/10.3390/agronomy12040954
Received: 8 March 2022 / Revised: 8 April 2022 / Accepted: 13 April 2022 / Published: 15 April 2022
(This article belongs to the Special Issue Cover Crops Contributions to the Soil Microbiome)
Cover cropping (CC) is the most promising in-field practice to improve soil health and mitigate N losses from fertilizer use. Although the soil microbiota play essential roles in soil health, their response to CC has not been well characterized by bioindicators of high taxonomic resolution within typical agricultural systems. Our objective was to fill this knowledge gap with genus-level indicators for corn [Zea mays L.] monocultures with three N fertilizer rates (N0, N202, N269; kg N ha−1), after introducing a CC mixture of cereal rye [Secale cereale L.] and hairy vetch [Vicia villosa Roth.], using winter fallows (BF) as controls. A 3 × 2 split-plot arrangement of N rates and CC treatments was studied in a randomized complete block design with three replicates over two years. Bacterial and archaeal 16S rRNA and fungal ITS regions were sequenced with Illumina MiSeq system. Overall, our high-resolution bioindicators were able to represent specific functional or ecological shifts within the microbial community. The abundances of indicators representing acidophiles, nitrifiers, and denitrifiers increased with N fertilization, while those of heterotrophic nitrifiers, nitrite oxidizers, and complete denitrifiers increased with N0. Introducing CC decreased soil nitrate levels by up to 50% across N rates, and CC biomass increased by 73% with N fertilization. CC promoted indicators of diverse functions and niches, including N-fixers, nitrite reducers, and mycorrhizae, while only two N-cycling genera were associated with BF. Thus, CC can enhance the soil biodiversity of simplified cropping systems and reduce nitrate leaching, but might increase the risk of nitrous oxide emission without proper nutrient management. This primary information is the first of its kind in this system and provided valuable insights into the limits and potential of CC as a strategy to improve soil health. View Full-Text
Keywords: bioinformatics; nitrogen cycling; soil microbiota; maize; nitrate leaching; nitrous oxide emission bioinformatics; nitrogen cycling; soil microbiota; maize; nitrate leaching; nitrous oxide emission
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MDPI and ACS Style

Kim, N.; Riggins, C.W.; Zabaloy, M.C.; Allegrini, M.; Rodriguez-Zas, S.L.; Villamil, M.B. High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monocultures. Agronomy 2022, 12, 954. https://doi.org/10.3390/agronomy12040954

AMA Style

Kim N, Riggins CW, Zabaloy MC, Allegrini M, Rodriguez-Zas SL, Villamil MB. High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monocultures. Agronomy. 2022; 12(4):954. https://doi.org/10.3390/agronomy12040954

Chicago/Turabian Style

Kim, Nakian, Chance W. Riggins, María C. Zabaloy, Marco Allegrini, Sandra L. Rodriguez-Zas, and María B. Villamil. 2022. "High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monocultures" Agronomy 12, no. 4: 954. https://doi.org/10.3390/agronomy12040954

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