Transcriptome Study of Rice Roots Status under High Alkaline Stress at Seedling Stage
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. RNA Extraction, Sequencing, and Mapping
2.3. Gene Ontology (GO) and KEGG Analysis
2.4. qRT-PCR Analysis
3. Results
3.1. Comparison of the Phenotypic Variation between Fourth Day-Stress and Seventh Day-Stress during Alkaline Stress
3.2. Gene Expression across Rice Genome during Alkaline Stress Process
3.3. Identification of Differentially Expressed Genes between Fourth Day-Stress and Seventh Day-Stress
3.4. Annotation and Function Classification of DEGs
3.5. Detection of Key Regulatory Genes under Alkaline Stress with Two Timepoints
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Wang, W.; Vinocur, B.; Altman, A. Plant responses to drought, salinity and extreme temperatures: Towards genetic engineering for stress tolerance. Planta 2003, 218, 1–14. [Google Scholar] [CrossRef] [PubMed]
- Zhao, X.Y.; Bian, X.Y.; Li, Z.X.; Wang, X.W.; Yang, C.P. Genetic stability analysis of introduced Betula pendula, Betula kirghisorum, and Betula pubescens families in saline-alkali soil of northeastern China. Scand. J. For. Res. 2014, 29, 639–649. [Google Scholar] [CrossRef]
- Ponce, K.S.; Guo, L.; Leng, Y.; Meng, L.; Ye, G. Advances in Sensing, Response and Regulation Mechanism of Salt Tolerance in Rice. Int. J. Mol. Sci. 2021, 22, 2254. [Google Scholar] [CrossRef]
- Fang, S.; Hou, X.; Liang, X. Response Mechanisms of Plants Under Saline-Alkali Stress. Front. Plant Sci. 2021, 12, 667458. [Google Scholar] [CrossRef] [PubMed]
- Oster, J.; Shainberg, I.; Abrol, I. Reclamation of Salt-Affected Soils. Agric. Drain. 1999, 38, 659–691. [Google Scholar] [CrossRef]
- Wang, X.; Ajab, Z.; Liu, C.; Hu, S.; Liu, J.; Guan, Q. Overexpression of transcription factor SlWRKY28 improved the tolerance of Populus davidiana × P. bolleana to alkaline salt stress. BMC Genet. 2020, 21, 103. [Google Scholar] [CrossRef]
- Zhang, K.; Tang, J.; Wang, Y.; Kang, H.; Zeng, J. The tolerance to saline–alkaline stress was dependent on the roots in wheat. Physiol. Mol. Biol. Plants 2020, 26, 947–954. [Google Scholar] [CrossRef] [PubMed]
- An, M.; Wang, X.; Chang, D.; Wang, S.; Hong, D.; Fan, H.; Wang, K. Application of compound material alleviates saline and alkaline stress in cotton leaves through regulation of the transcriptome. BMC Plant Biol. 2020, 20, 462. [Google Scholar] [CrossRef]
- Wu, J.; Zhao, Q.; Wu, G.; Yuan, H.; Ma, Y.; Lin, H.; Pan, L.; Li, S.; Sun, D. Comprehensive Analysis of Differentially Expressed Unigenes under NaCl Stress in Flax (Linum usitatissimum L.) Using RNA-Seq. Int. J. Mol. Sci. 2019, 20, 20369. [Google Scholar] [CrossRef] [Green Version]
- Wang, Y.; Wang, J.; Zhao, X.; Yang, S.; Huang, L.; Du, F.; Li, Z.; Zhao, X.; Fu, B.; Wang, W. Overexpression of the Transcription Factor Gene OsSTAP1 Increases Salt Tolerance in Rice. Rice 2020, 13, 50. [Google Scholar] [CrossRef]
- Wu, G.; Li, Z.; Cao, H.; Wang, J. Genome-wide identification and expression analysis of the WRKY genes in sugar beet (Beta vulgaris L.) under alkaline stress. PeerJ 2019, 7, e7817. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ma, Q.; Xia, Z.; Cai, Z.; Li, L.; Cheng, Y.; Liu, J.; Nian, H. GmWRKY16 Enhances Drought and Salt Tolerance Through an ABA-Mediated Pathway in Arabidopsis thaliana. Front. Plant Sci. 2019, 9, 1979. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Liu, D.; Liu, M.; Liu, X.; Cheng, X.; Liang, Z. Silicon Priming Created an Enhanced Tolerance in Alfalfa (Medicago sativa L.) Seedlings in Response to High Alkaline Stress. Front. Plant Sci. 2018, 9, 716. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Yu, Z.; Duan, X.; Luo, L.; Dai, S.; Ding, Z.; Xia, G. How Plant Hormones Mediate Salt Stress Responses. Trends Plant Sci. 2020, 25, 1117–1130. [Google Scholar] [CrossRef]
- Verma, V.; Ravindran, P.; Kumar, P.P. Plant hormone-mediated regulation of stress responses. BMC Plant Biol. 2016, 16, 86. [Google Scholar] [CrossRef] [Green Version]
- Guo, M.; Wang, R.; Wang, J.; Hua, K.; Wang, Y.; Liu, X.; Yao, S. ALT1, a Snf2 Family Chromatin Remodeling ATPase, Negatively Regulates Alkaline Tolerance through Enhanced Defense against Oxidative Stress in Rice. PLoS ONE 2014, 9, e112515. [Google Scholar] [CrossRef] [Green Version]
- Li, J.; Xu, H.; Liu, W.; Zhang, X.; Lu, Y. Ethylene Inhibits Root Elongation during Alkaline Stress through AUXIN1 and Associated Changes in Auxin Accumulation. Plant Physiol. 2015, 168, 1777–1791. [Google Scholar] [CrossRef] [Green Version]
- Lee, S.; Kim, S.G.; Park, C.M. Salicylic acid promotes seed germination under high salinity by modulating antioxidant activity in Arabidopsis. New Phytol 2010, 188, 626–637. [Google Scholar] [CrossRef]
- Chauhan, P.S.; Lata, C.; Tiwari, S.; Chauhan, A.S.; Mishra, S.K.; Agrawal, L.; Chakrabarty, D.; Nautiyal, C.S. Transcriptional alterations reveal Bacillus amyloliquefaciens-rice cooperation under salt stress. Sci. Rep. 2019, 9, 11912. [Google Scholar] [CrossRef]
- Uozumi, N.; Kim, E.J.; Rubio, F.; Yamaguchi, T.; Muto, S.; Tsuboi, A.; Bakker, E.P.; Nakamura, T.; Schroeder, J.I. The Arabidopsis HKT1 gene homolog mediates inward Na(+) currents in xenopus laevis oocytes and Na(+) uptake in Saccharomyces cerevisiae. Plant Physiol. 2000, 122, 1249–1259. [Google Scholar] [CrossRef] [Green Version]
- Ren, Z.H.; Gao, J.P.; Li, L.G.; Cai, X.L.; Huang, W.; Chao, D.Y.; Zhu, M.Z.; Wang, Z.Y.; Luan, S.; Lin, H.X. A rice quantitative trait locus for salt tolerance encodes a sodium transporter. Nat. Genet. 2005, 37, 1141–1146. [Google Scholar] [CrossRef]
- He, Y.; Dong, Y.; Yang, X.; Guo, D.; Qian, X.; Yan, F.; Wang, Y.; Li, J.; Wang, Q. Functional activation of a novel R2R3-MYB protein gene, GmMYB68, confers salt-alkali resistance in soybean (Glycine max L.). Genome 2020, 63, 13–26. [Google Scholar] [CrossRef] [PubMed]
- Lu, X.; Zhang, X.; Duan, H.; Lian, C.; Liu, C.; Yin, W.; Xia, X. Three stress-responsive NAC transcription factors from Populus euphratica differentially regulate salt and drought tolerance in transgenic plants. Physiol. Plant 2018, 162, 73–97. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Guo, M.; Li, S.; Tian, S.; Wang, B.; Zhao, X. Transcriptome analysis of genes involved in defense against alkaline stress in roots of wild jujube (Ziziphus acidojujuba). PLoS ONE 2017, 12, e185732. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- An, Y.M.; Song, L.L.; Liu, Y.R.; Shu, Y.J.; Guo, C.H. De Novo Transcriptional Analysis of Alfalfa in Response to Saline-Alkaline Stress. Front. Plant Sci. 2016, 7, 931. [Google Scholar] [CrossRef] [Green Version]
- Wang, J.; Zhang, Y.; Yan, X.; Guo, J. Physiological and transcriptomic analyses of yellow horn (Xanthoceras sorbifolia) provide important insights into salt and saline-alkali stress tolerance. PLoS ONE 2020, 15, e244365. [Google Scholar] [CrossRef]
- Shah, W.H.; Rasool, A.; Saleem, S.; Mushtaq, N.U.; Tahir, I.; Hakeem, K.R.; Rehman, R.U. Understanding the Integrated Pathways and Mechanisms of Transporters, Protein Kinases, and Transcription Factors in Plants under Salt Stress. Int. J. Genom. 2021, 2021, 1–16. [Google Scholar] [CrossRef]
- Zhang, P.; Duo, T.; Wang, F.; Zhang, X.; Yang, Z.; Hu, G. De novo transcriptome in roots of switchgrass (Panicum virgatum L.) reveals gene expression dynamic and act network under alkaline salt stress. BMC Genom. 2021, 22, 82. [Google Scholar] [CrossRef]
- Ruzicka, K.; Ljung, K.; Vanneste, S.; Podhorska, R.; Beeckman, T.; Friml, J.; Benkova, E. Ethylene regulates root growth through effects on auxin biosynthesis and transport-dependent auxin distribution. Plant Cell 2007, 19, 2197–2212. [Google Scholar] [CrossRef] [Green Version]
- Wang, R.; Jing, W.; Xiao, L.; Jin, Y.; Shen, L.; Zhang, W. The Rice High-Affinity Potassium Transporter1;1 Is Involved in Salt Tolerance and Regulated by an MYB-Type Transcription Factor. Plant Physiol. 2015, 168, 1076–1090. [Google Scholar] [CrossRef] [Green Version]
- Zhou, Y.; Yang, P.; Cui, F.; Zhang, F.; Luo, X.; Xie, J. Transcriptome Analysis of Salt Stress Responsiveness in the Seedlings of Dongxiang Wild Rice (Oryza rufipogon Griff.). PLoS ONE 2016, 11, e146242. [Google Scholar] [CrossRef] [Green Version]
- Liu, J.; Wang, Y.; Li, Q. Analysis of differentially expressed genes and adaptive mechanisms of Prunus triloba Lindl. under alkaline stress. Hereditas 2017, 154, 10. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Li, N.; Liu, H.; Sun, J.; Zheng, H.; Wang, J.; Yang, L.; Zhao, H.; Zou, D. Transcriptome analysis of two contrasting rice cultivars during alkaline stress. Sci. Rep. 2018, 8, 9586. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Li, Q.; Yang, A.; Zhang, W. Comparative studies on tolerance of rice genotypes differing in their tolerance to moderate salt stress. BMC Plant Biol. 2017, 17, 141. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Li, Q.; Ma, C.; Tai, H.; Qiu, H.; Yang, A. Comparative transcriptome analysis of two rice genotypes differing in their tolerance to saline-alkaline stress. PLoS ONE 2020, 15, e243112. [Google Scholar] [CrossRef] [PubMed]
- Lv, B.S.; Li, X.W.; Ma, H.Y.; Sun, Y.; Wei, L.X.; Jiang, C.J.; Liang, Z.W. Differences in Growth and Physiology of Rice in Response to Different Saline-Alkaline Stress Factors. Agron. J. 2013, 105, 1119–1128. [Google Scholar] [CrossRef]
- Chong, C.; Li, Y.; Wang, T.; Chang, H. Stratification of adverse outcomes by preoperative risk factors in coronary artery bypass graft patients: An artificial neural network prediction model. AMIA Annu. Symp. Proc. 2003, 2003, 160–164. [Google Scholar]
- Li, H.; Durbin, R. Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics 2009, 25, 1754–1760. [Google Scholar] [CrossRef] [Green Version]
- Dobin, A.; Davis, C.A.; Schlesinger, F.; Drenkow, J.; Zaleski, C.; Jha, S.; Batut, P.; Chaisson, M.; Gingeras, T.R. STAR: Ultrafast universal RNA-seq aligner. Bioinformatics 2013, 29, 15–21. [Google Scholar] [CrossRef]
- Kawahara, Y.; de la Bastide, M.; Hamilton, J.P.; Kanamori, H.; Mccombie, W.R.; Ouyang, S.; Schwartz, D.C.; Tanaka, T.; Wu, J.; Zhou, S.; et al. Improvement of the Oryza sativa Nipponbare reference genome using next generation sequence and optical map data. Rice 2013, 6, 4. [Google Scholar] [CrossRef] [Green Version]
- Wang, N.; Zhang, D.; Wang, Z.; Xun, H.; Ma, J.; Wang, H.; Huang, W.; Liu, Y.; Lin, X.; Li, N.; et al. Mutation of the RDR1 gene caused genome-wide changes in gene expression, regional variation in small RNA clusters and localized alteration in DNA methylation in rice. BMC Plant Biol. 2014, 14, 177. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Chang, W.; Niu, Y.; Yu, M.; Li, T.; Li, J.; Lu, K. qPrimerDB: A Powerful and User-Friendly Database for qPCR Primer Design. Methods Mol. Biol. 2022, 2392, 173–182. [Google Scholar] [CrossRef] [PubMed]
- Fukuda, A.; Nakamura, A.; Hara, N.; Toki, S.; Tanaka, Y. Molecular and functional analyses of rice NHX-type Na+/H+ antiporter genes. Planta 2011, 233, 175–188. [Google Scholar] [CrossRef] [PubMed]
- Horie, T.; Sugawara, M.; Okada, T.; Taira, K.; Kaothien-Nakayama, P.; Katsuhara, M.; Shinmyo, A.; Nakayama, H. Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells. J. Biosci. Bioeng. 2011, 111, 346–356. [Google Scholar] [CrossRef]
- He, Y.; Yang, B.; He, Y.; Zhan, C.; Cheng, Y.; Zhang, J.; Zhang, H.; Cheng, J.; Wang, Z. A quantitative trait locus, qSE3, promotes seed germination and seedling establishment under salinity stress in rice. Plant J. 2019, 97, 1089–1104. [Google Scholar] [CrossRef] [Green Version]
- Yang, C.W.; Wang, P.; Li, C.Y.; Shi, D.C.; Wang, D.L. Comparison of effects of salt and alkali stresses on the growth and photosynthesis of wheat. Photosynthetica 2008, 46, 107–114. [Google Scholar] [CrossRef]
- Zhang, B.; Chen, X.; Lu, X.; Shu, N.; Wang, X.; Yang, X.; Wang, S.; Wang, J.; Guo, L.; Wang, D.; et al. Transcriptome Analysis of Gossypium hirsutum L. Reveals Different Mechanisms among NaCl, NaOH and Na2CO3 Stress Tolerance. Sci. Rep. 2018, 8, 13527. [Google Scholar] [CrossRef]
- Li, Q.; Yang, A.; Zhang, W. Efficient acquisition of iron confers greater tolerance to saline-alkaline stress in rice (Oryza sativa L.). J. Exp. Bot. 2016, 67, 6431–6444. [Google Scholar] [CrossRef] [Green Version]
- Lu, X.; Li, F.; Ma, X.; Jing, P.; Luo, C.; Tian, L.; Li, P. Physiological response strategies of roots of different alkali-tolerant rice varieties to alkali stress. Chin. J. Ecol. Agric. 2021, 7, 1171–1184. [Google Scholar] [CrossRef]
- Zhang, H.; Liu, X.; Zhang, R.; Yuan, H.; Wang, M.; Yang, H.; Ma, H.; Liu, D.; Jiang, C.; Liang, Z. Root Damage under Alkaline Stress Is Associated with Reactive Oxygen Species Accumulation in Rice (Oryza sativa L.). Front. Plant Sci. 2017, 8, 1580. [Google Scholar] [CrossRef]
- Wang, H.; Wu, Z.; Han, J.; Zheng, W.; Yang, C.; Niedz, R.P. Comparison of ion balance and nitrogen metabolism in old and young leaves of alkali-stressed rice plants. PLoS ONE 2012, 7, e37817. [Google Scholar] [CrossRef] [PubMed]
- Elmore, S. Apoptosis: A Review of Programmed Cell Death. Toxicol. Pathol. 2007, 35, 495–516. [Google Scholar] [CrossRef]
- Singh, A.K.; Dhanapal, S.; Yadav, B.S. The dynamic responses of plant physiology and metabolism during environmental stress progression. Mol. Biol. Rep. 2020, 47, 1459–1470. [Google Scholar] [CrossRef] [PubMed]
- An, Y.; Liang, Z. Staged strategy of plants in response to drought stress. Chin. J. Appl. Ecol. 2012, 23, 2907–2915. [Google Scholar] [CrossRef]
- Zhang, C.; Shi, S.; Wu, F. Effects of Drought Stress on Root and Physiological Responses of Different Drought-Tolerant Alfalfa Varieties. Sci. Agric. Sin. 2018, 51, 868–882. [Google Scholar] [CrossRef]
- Wu, Y.; Lin, F.; Zhou, Y.; Wang, J.; Sun, S.; Wang, B.; Zhang, Z.; Li, G.; Lin, X.; Wang, X.; et al. Genomic mosaicism due to homoeologous exchange generates extensive phenotypic diversity in nascent allopolyploids. Natl. Sci. Rev. 2021, 8. [Google Scholar] [CrossRef] [PubMed]
- Hu, L.; Li, N.; Xu, C.; Zhong, S.; Lin, X.; Yang, J.; Zhou, T.; Yuliang, A.; Wu, Y.; Chen, Y.R.; et al. Mutation of a major CG methylase in rice causes genome-wide hypomethylation, dysregulated genome expression, and seedling lethality. Proc. Natl. Acad. Sci. USA 2014, 111, 10642–10647. [Google Scholar] [CrossRef] [Green Version]
- Marconi, G.; Pace, R.; Traini, A.; Raggi, L.; Lutts, S.; Chiusano, M.; Guiducci, M.; Falcinelli, M.; Benincasa, P.; Albertini, E. Use of MSAP Markers to Analyse the Effects of Salt Stress on DNA Methylation in Rapeseed (Brassica napus var. oleifera). PLoS ONE 2013, 8, e75597. [Google Scholar] [CrossRef] [Green Version]
- Viggiano, L.; Pinto, M. Dynamic DNA Methylation Patterns in Stress Response. In Plant Epigenetics; Springer: Berlin/Heidelberg, Germany, 2017; pp. 281–302. [Google Scholar] [CrossRef]
- Abd-Hamid, N.; Ahmad-Fauzi, M.; Zainal, Z.; Ismail, I. Diverse and dynamic roles of F-box proteins in plant biology. Planta 2020, 251, 68. [Google Scholar] [CrossRef] [Green Version]
- Jing, S.; Zhou, X.; Song, Y.; Yu, D. Heterologous expression of OsWRKY23 gene enhances pathogen defense and dark-induced leaf senescence in Arabidopsis. Plant Growth Regul. 2009, 58, 181–190. [Google Scholar] [CrossRef]
- Gao, F.; Xiong, A.; Peng, R.; Jin, X.; Xu, J.; Zhu, B.; Chen, J.; Yao, Q. OsNAC52, a rice NAC transcription factor, potentially responds to ABA and confers drought tolerance in transgenic plants. Plant Cell Tissue Organ Cult. 2010, 100, 255–262. [Google Scholar] [CrossRef]
- Hu, S.; Yu, Y.; Chen, Q.; Mu, G.; Shen, Z.; Zheng, L. OsMYB45 plays an important role in rice resistance to cadmium stress. Plant Sci. 2017, 264, 1–8. [Google Scholar] [CrossRef] [PubMed]
- Zhang, S.; Haider, I.; Kohlen, W.; Jiang, L.; Bouwmeester, H.; Meijer, A.H.; Schluepmann, H.; Liu, C.; Ouwerkerk, P.B.F. Function of the HD-Zip I gene Oshox22 in ABA-mediated drought and salt tolerances in rice. Plant Mol. Biol. 2012, 80, 571–585. [Google Scholar] [CrossRef]
- Zou, M.; Guan, Y.; Ren, H.; Zhang, F.; Chen, F. A bZIP transcription factor, OsABI5, is involved in rice fertility and stress tolerance. Plant Mol. Biol. 2008, 66, 675–683. [Google Scholar] [CrossRef] [PubMed]
- Pandian, B.A.; Sathishraj, R.; Djanaguiraman, M.; Prasad, P.; Jugulam, M. Role of Cytochrome P450 Enzymes in Plant Stress Response. Antioxidants 2020, 9, 454. [Google Scholar] [CrossRef] [PubMed]
- Das, A.; Pramanik, K.; Sharma, R.; Gantait, S.; Banerjee, J. In-silico study of biotic and abiotic stress-related transcription factor binding sites in the promoter regions of rice germin-like protein genes. PLoS ONE 2019, 14, e211887. [Google Scholar] [CrossRef] [PubMed]
- Manosalva, P.M.; Davidson, R.M.; Liu, B.; Zhu, X.; Hulbert, S.H.; Leung, H.; Leach, J.E. A Germin-Like Protein Gene Family Functions as a Complex Quantitative Trait Locus Conferring Broad-Spectrum Disease Resistance in Rice. Plant Physiol. 2009, 149, 286–296. [Google Scholar] [CrossRef] [Green Version]
- Li, L.; Xu, X.; Chen, C.; Shen, Z. Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis. Int. J. Mol. Sci. 2016, 17, 1622. [Google Scholar] [CrossRef] [Green Version]
- De Zaeytijd, J.; Chen, P.; Scheys, F.; Subramanyam, K.; Dubiel, M.; De Schutter, K.; Smagghe, G.; Van Damme, E.J. Involvement of OsRIP1, a ribosome-inactivating protein from rice, in plant defense against Nilaparvata lugens. Phytochemistry 2020, 170, 112190. [Google Scholar] [CrossRef]
- Wu, C.; Zhou, S.; Zhang, Q.; Zhao, W.; Peng, Y. Molecular cloning and differential expression of an γ-aminobutyrate transaminase gene, OsGABA-T, in rice (Oryza sativa) leaves infected with blast fungus. J. Plant Res. 2006, 119, 663–669. [Google Scholar] [CrossRef]
- Perrin, R.M.; Derocher, A.E.; Bar-Peled, M.; Zeng, W.; Norambuena, L.; Orellana, A.; Raikhel, N.V.; Keegstra, K. Xyloglucan Fucosyltransferase, an Enzyme Involved in Plant Cell Wall Biosynthesis. Science 1999, 284, 1976–1979. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Zhou, L.; Cheung, M.; Li, M.; Fu, Y.; Sun, Z.; Sun, S.; Lam, H. Rice Hypersensitive Induced Reaction Protein 1 (OsHIR1) associates with plasma membrane and triggers hypersensitive cell death. BMC Plant Biol. 2010, 10, 290. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Zhou, L.; Cheung, M.; Zhang, Q.I.; Lei, C.; Zhang, S.; Sun, S.S.; Lam, H. A novel simple extracellular leucine-rich repeat (eLRR) domain protein from rice (OsLRR1) enters the endosomal pathway and interacts with the hypersensitive-induced reaction protein 1 (OsHIR1). Plant Cell Environ. 2009, 32, 1804–1820. [Google Scholar] [CrossRef] [PubMed]
- Dubey, A.; Malla, M.A.; Kumar, A.; Dayanandan, S.; Khan, M.L. Plants endophytes: Unveiling hidden agenda for bioprospecting toward sustainable agriculture. Crit. Rev. Biotechnol. 2020, 40, 1210–1231. [Google Scholar] [CrossRef]
- Xiao, B.; Huang, Y.; Tang, N.; Xiong, L. Over-expression of a LEA gene in rice improves drought resistance under the field conditions. Theor. Appl. Genet. 2007, 115, 35–46. [Google Scholar] [CrossRef]
- Wang, C.; Guo, W.; Cai, X.; Li, R.; Ow, D.W. Engineering low-cadmium rice through stress-inducible expression of OXS3-family member genes. New Biotechnol. 2019, 48, 29–34. [Google Scholar] [CrossRef]
- Sato, Y.; Morita, R.; Katsuma, S.; Nishimura, M.; Tanaka, A.; Kusaba, M. Two short-chain dehydrogenase/reductases, NON-YELLOW COLORING 1 and NYC1-LIKE, are required for chlorophyll b and light-harvesting complex II degradation during senescence in rice. Plant J. 2009, 57, 120–131. [Google Scholar] [CrossRef]
- Wu, H.; Wang, B.; Chen, Y.; Liu, Y.; Chen, L. Characterization and fine mapping of the rice premature senescence mutant ospse1. Theor. Appl. Genet. 2013, 126, 1897–1907. [Google Scholar] [CrossRef]
- Solomon, M.; Belenghi, B.; Delledonne, M.; Menachem, E.; Levine, A. The Involvement of Cysteine Proteases and Protease Inhibitor Genes in the Regulation of Programmed Cell Death in Plants. Plant Cell 1999, 11, 431–443. [Google Scholar] [CrossRef]
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Lin, Y.; Ma, J.; Wu, N.; Qi, F.; Peng, Z.; Nie, D.; Yao, R.; Qi, X.; Slaski, J.; Yang, F.; et al. Transcriptome Study of Rice Roots Status under High Alkaline Stress at Seedling Stage. Agronomy 2022, 12, 925. https://doi.org/10.3390/agronomy12040925
Lin Y, Ma J, Wu N, Qi F, Peng Z, Nie D, Yao R, Qi X, Slaski J, Yang F, et al. Transcriptome Study of Rice Roots Status under High Alkaline Stress at Seedling Stage. Agronomy. 2022; 12(4):925. https://doi.org/10.3390/agronomy12040925
Chicago/Turabian StyleLin, Yujie, Jian Ma, Nan Wu, Fan Qi, Zhanwu Peng, Dandan Nie, Rongrong Yao, Xin Qi, Jan Slaski, Fu Yang, and et al. 2022. "Transcriptome Study of Rice Roots Status under High Alkaline Stress at Seedling Stage" Agronomy 12, no. 4: 925. https://doi.org/10.3390/agronomy12040925